Lytic potential of Lysobacter capsici VKM B-2533T: bacteriolytic enzymes and outer membrane vesicles

Abstract

Recent recurrent outbreaks of bacterial resistance to antibiotics have shown the critical need to identify new lytic agents to combat them. The species Lysobacter capsici VKM B-2533T possesses a potent antimicrobial action against a number of bacteria, fungi and yeasts. Its activity can be due to the impact of bacteriolytic enzymes, antibiotics and peptides. This work isolated four homogeneous bacteriolytic enzymes and a mixture of two proteins, which also had a bacteriolytic activity. The isolates included proteins identical to L. enzymogenes α- and β-lytic proteases and lysine-specific protease. The proteases of 26 kDa and 29 kDa and a protein identified as N-acetylglycosaminidase had not been isolated in Lysobacter earlier. The isolated β-lytic protease digested live methicillin-resistant staphylococcal cells with high efficiency (minimal inhibitory concentration, 2.85 μg/mL). This property makes the enzyme deserving special attention. A recombinant β-lytic protease was produced. The antimicrobial potential of the bacterium was contributed to by outer membrane vesicles (OMVs). L. capsici cells were found to form a group of OMVs responsible for antifungal activity. The data are indicative of a significant antimicrobial potential of this bacterium that requires thorough research.

Lysobacter penaei sp. nov., isolated from intestinal content of a Pacific white shrimp (Penaeus vannamei)

“…Strains of the genus Lysobacter are Gram-stain-negative, non-flagellated and flexing thin rods forming mucoid colonies [1]. Researchers are very interested in Lysobacter strains for their potential of producing mycolytic and bacteriolytic enzymes and antibiotic compounds [2–5]. Christensen and Cook first established the genus Lysobacter in 1978 [6] and, at the time of writing, this genus currently comprises 61 validly published and 12 invalidly published species (LSPN; www.bacterio.net) .…”

Deletion of alpB Gene Influences Outer Membrane Vesicles Biogenesis of Lysobacter sp. XL1

“…Data supporting a model for the specific sorting of vesicle components emerged (Horstman and Kuehn, 2000;Kato et al, 2002;Haurat et al, 2011Haurat et al, , 2015Schwechheimer and Kuehn, 2015). This contributed to the emergence of the theory of the formation of various vesicle groups (subpopulations) produced by one cell type (Olofsson et al, 2010;Rompikuntal et al, 2012;Kunsmann et al, 2015;Kudryakova et al, 2015;Afoshin et al, 2020). It should be noted that this theory enables understanding the ability of vesicles to perform various vital activities of the bacterial cell.…”

Draft Genome Sequence of the Type Strain Lysobacter capsici VKM B-2533

“…Bacteria of the genus Lysobacter are unique producers of various lytic agents (bacteriolytic enzymes, antibiotics, and peptides) ( 1 – 7 ). In this regard, Lysobacter spp.…”

“… Lysobacter capsici VKM B-2533 T (=KCTC 22007 T = DSM 19286 T ) was isolated from the rhizosphere of pepper at the Gyeongsang National University (Jinju, South Korea) in 2008 ( 8 ). This strain was shown to have potent antifungal and antibacterial effects ( 7 – 9 ). An antifungal effect against Phytophthora infestans was observed in volatile organic compounds found in the strain ( 10 ).…”

“…An antifungal effect against Phytophthora infestans was observed in volatile organic compounds found in the strain ( 10 ). Bacteriolytic enzymes were also purified, among them β-lytic protease, which demonstrated potent antistaphylococcal activity ( 7 ). Here, we report a draft genome sequence of L. capsici VKM B-2533 T .…”