Lysobacter penaei sp. nov., isolated from intestinal content of a Pacific white shrimp (Penaeus vannamei)

Abstract

The polyphasic taxonomic approach was used to characterize a novel bacteria strain, designated SG-8T, which was isolated from intestinal content of a Pacific white shrimp (Penaeus vannamei). Cells were Gram-stain-negative, aerobic, non-gliding rods. Growth occurred at 10–45 °C (optimum, 20–30 °C), pH 5.0–10.0 (optimum, 6.0–7.0) and in 0–6.0 % (w/v) NaCl (optimum, 0–4.0 %). The 16S rRNA gene sequence of strain SG-8T showed the highest sequence similarity to Lysobacter maris KMU-14T (98.6 %). On phylogenetic trees, strain SG-8T formed a stable cluster with Lysobacter maris KMU-14T, Lysobacter alkalisoli SJ-36T, Lysobacter spongiae 119BY6-57T and Lysobacter aestuarii S2-CT. The average nucleotide identity and digital DNA–DNA hybridization values between strain SG-8T and the four reference type strains listed above were 83.3, 82.3, 83.5, 83.3% and 22.8, 22.7, 22.7, 22.9 %, respectively. The major fatty acids (>5 %) were iso-C15 : 0, summed feature 9 (iso-C17 : 1 ω9c and/or 10-methyl C16 : 0), iso-C16 : 0, summed feature 3 (C16 : 1 ω6c and/or C16 : 1 ω7c), iso-C17 : 0, iso-C11 : 0 3OH and iso-C11 : 0. Ubiquinone-8 (Q-8) was the only respiratory quinone. The major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol and phosphatidylglycerol. The DNA G+C content was 68.8 mol%. Based on the results of genomic, phylogenetic, phenotypic and chemotaxonomic analyses, strain SG-8T represents a novel species of the genus Lysobacter , for which the name Lysobacter penaei sp. nov. is proposed. The type strain is SG-8T (=GDMCC 1.1817T=KACC 21942T).

Bacteria Associated With Phaeocystis globosa and Their Influence on Colony Formation

“…In addition, the most dominant bacterial genus, Marinobacter , as identified in this study, was not the most dominant one in previous studies which used P . globosa strains differ from the current study ( Xu et al, 2021a , b ; Zhu et al, 2021 ). These results suggest that different P .…”

“…Plates were then incubated at 20°C for 7 days in the dark. Bacterial colonies exhibiting different morphological characteristics were isolated and stored in marine broth 2216 (MB, BD Difco) supplemented with 20% glycerol to form stocks that were stored at −80°C for future experiments ( Xu et al, 2021a , b ).…”

Parvicella tangerina gen. nov., sp. nov. (Parvicellaceae fam. nov., Flavobacteriales), first cultured representative of the marine clade UBA10066, and Lysobacter luteus sp. nov., from activated sludge of a seawater-processing wastewater treatment plant

“…Characteristics differentiating Lysobacter luteus CECT 30171 T from other Lysobacter species Strains: 1, L. luteus sp. nov. CECT 30171 T (this study); 2, L. arseniciresistens[51]; 3, L. concretionis[52]; 4, L. spongiae[53]; 5, L. defluvii[54]; 6, L. spongiicola[55]; 7, L. aestuarii[56]; 8, L. maris[57]; 9, L. penaei[58]; 10, L. alkalisoli[59]; 11, L. enzymogenes[7]. +, Positive; −, negative; v, variable; nd, not determined; Y, yellow; O, orange; PG, phosphatidylglycerol; PE, phosphatidylethanolamine.…”