Lytic KFS-SE2 phage as a novel bio-receptor for Salmonella Enteritidis detection

Choi, In Young; Lee, Cheonghoon; Song, Won Keun; Jang, Sung Jae; Park, Mi Kyung

doi:10.1007/s12275-019-8610-0

Cited by 11 publications

(12 citation statements)

References 67 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…An aliquot of 10 µL of the filtrate was dotted on the surface of pre-solidified TA soft agar (4 g agar, 8 g nutrient broth, 5 g NaCl, 0.2 g MgSO 4 , 0.05 g MnSO 4 , and 0.15 g CaCl 2 per 1 L) containing 200 µL of the indicator strain suspension. The presence of phages in the filtrate was then confirmed using a dot assay [ 9 ]. Once the formation of a clear zone was observed, a plaque assay was performed to isolate single phages from the filtrate.…”

Section: Methodsmentioning

confidence: 99%

“…After incubation for 16 h at 37°C, each plaque was picked with a customized tip and eluted with sodium chloride-magnesium sulphate (SM) buffer (50 mM Tris-HCl, 100 mM NaCl, 10 mM MgSO 4 , pH 7.5) with vigorous agitation for 1 h at 22°C. All of these procedures were repeated until the single phage was isolated [ 9 ]. To increase of the concentration of the single phage, 3 mL of TA broth containing 1% (v/v) indicator strain suspension was incubated for 2.5 h at 37°C.…”

Section: Methodsmentioning

confidence: 99%

“…Most phages (approximately 90%) that are isolated from the environment are lytic and tailed phages [9]. Although a lytic property is necessary for identifying and purifying of phages from the environment, it is not always beneficial for use as a bio-receptor.…”

Section: One-step Growth Curve Of Kfs-stmentioning

confidence: 99%

“…Thus, the Korean government has implemented the HACCP (Hazard Analysis and Critical Control Point) for the pork industry in 2006, beef industry in 2007 and poultry industry in 2008 for the control of Salmonella [5,8]. Therefore, more rapid, sensitive, specific, simple, and hand-held detection methods are required for on-site applicable Salmonella detection [9].…”

Section: Introductionmentioning

confidence: 99%

“…Phage (bacteriophage) has been gaining more attentions over several bio-receptors including antibodies, cell, enzyme, and DNA. Phages possess excellent specificity, robustness under harsh conditions (pH, temperature), safety, and relatively cheap and easy production processes [9]. Phages, as a natural predator of bacteria, are viruses that infect the bacteria cell specifically.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Exploring the feasibility of Salmonella Typhimurium-specific phage as a novel bio-receptor

Choi¹,

Park²,

Chin³

et al. 2020

J Anim Sci Technol

Self Cite

View full text Add to dashboard Cite

The purpose of this study was aimed to isolate a Salmonella Typhimurium-specific phage (KFS-ST) from washing water in a poultry processing facility and to investigate the feasibility of the KFS-ST as a novel bio-receptor for the magnetoelastic (ME) biosensor method. KFS-ST against S. Typhimurium was isolated, propagated, and purified using a CsCl-gradient ultracentrifugation. Morphological characteristics of KFS-ST were analyzed using transmission electron microscopy (TEM). Its specificity and efficiency of plating analysis were conducted against 39 foodborne pathogens. The temperature and pH stabilities of KFS-ST were investigated by the exposure of the phage to various temperatures (−70℃-70℃) and pHs (1-12) for 1 h. A one-step growth curve analysis was performed to determine the eclipse time, latent time and burst size of phage. The storage stability of KFS-ST was studied by exposing KFS-ST to various storage temperatures (−70℃, −20℃, 4℃, and 22℃) for 12 weeks. KFS-ST was isolated and purified with a high concentration of (11.47 ± 0.25) Log PFU/mL. It had an icosahedral head (56.91 ± 2.90 nm) and a non-contractile tail (225.49 ± 2.67 nm), which was classified into the family of Siphoviridae in the order of Caudovirales. KFS-ST exhibited an excellent specificity against only S. Typhimurium and S. Enteritidis, which are considered two of the most problematic Salmonella strains in the meat and poultry. However, KFS-ST did not exhibit any specificity against six other Salmonella and 27 non-Salmonella strains. KFS-ST was stable at temperature of 4℃ to 50℃ and at pH of 4 to 12. The eclipse time, latent time, and burst size of KFS-ST were determined to be 10 min, 25 min and 26 PFU/ infected cell, respectively. KFS-ST was relatively stable during the 12-week storage period at all tested temperatures. Therefore, this study demonstrated the feasibility of KFS-ST as a novel bio-receptor for the detection of S. Typhimurium and S. Enteritidis in meat and poultry products using the ME biosensor method.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: One-step Growth Curve Of Kfs-stmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Exploring the feasibility of Salmonella Typhimurium-specific phage as a novel bio-receptor

Choi¹,

Park²,

Chin³

et al. 2020

J Anim Sci Technol

Self Cite

View full text Add to dashboard Cite

show abstract

Breathing‐Driven Self‐Powered Pyroelectric ZnO Integrated Face Mask for Bioprotection

Kim

Song

Lee

et al. 2022

Small

View full text Add to dashboard Cite

Rapid spread of infectious diseases is a global threat and has an adverse impact on human health, livelihood, and economic stability, as manifested in the ongoing coronavirus disease 2019 (COVID‐19) pandemic. Even though people wear a face mask as protective equipment, direct disinfection of the pathogens is barely feasible, which thereby urges the development of biocidal agents. Meanwhile, repetitive respiration generates temperature variation wherein the heat is regrettably wasted. Herein, a biocidal ZnO nanorod‐modified paper (ZNR‐paper) composite that is 1) integrated on a face mask, 2) harvests waste breathing‐driven thermal energy, 3) facilitates the pyrocatalytic production of reactive oxygen species (ROS), and ultimately 4) exhibits antibacterial and antiviral performance is proposed. Furthermore, in situ generated compressive/tensile strain of the composite by being attached to a curved mask is investigated for high pyroelectricity. The anisotropic ZNR distortion in the bent composite is verified with changes in ZnO bond lengths and OZnO bond angles in a ZnO4 tetrahedron, resulting in an increased polarization state and possibly contributing to the following pyroelectricity. The enhanced pyroelectric behavior is demonstrated by efficient ROS production and notable bioprotection. This study exploring the pre‐strain effect on the pyroelectricity of ZNR‐paper might provide new insights into the piezo‐/pyroelectric material‐based applications.

show abstract

Salmonella phage akira, infecting selected Salmonella enterica Enteritidis and Typhimurium strains, represents a new lineage of bacteriophages

Olsen

Lametsch

Wagner³

et al. 2022

Arch Virol

View full text Add to dashboard Cite

Lytic KFS-SE2 phage as a novel bio-receptor for Salmonella Enteritidis detection

Cited by 11 publications

References 67 publications

Exploring the feasibility of Salmonella Typhimurium-specific phage as a novel bio-receptor

Exploring the feasibility of Salmonella Typhimurium-specific phage as a novel bio-receptor

Breathing‐Driven Self‐Powered Pyroelectric ZnO Integrated Face Mask for Bioprotection

Salmonella phage akira, infecting selected Salmonella enterica Enteritidis and Typhimurium strains, represents a new lineage of bacteriophages

Contact Info

Product

Resources

About