Lysozymuria Induced in Rats by Nephrotoxic Agents

Balázs, T.; Roepke, Raymond R.

doi:10.3181/00379727-123-31494

Cited by 30 publications

(17 citation statements)

References 2 publications

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“…The use of 'foreign' muramidase enables the concentration of muramidase within the proximal tubular cell of the rat kidney to be seen as the reabsorption of filtered protein, rather than renal synthesis of muramidase as has been suggested (Balazs and Roepke, 1966). In a recent study using perfused rat kidney, Maack and Sigulem (1974) have demonstrated that rat muramidase is treated as a filtrable molecule, with characteristics of renal handling very close to those found with human muramidase in the present study.…”

Section: Muramidasesupporting

confidence: 74%

Effect of human lysozyme (muramidase) on potassium handling by the perfused rat kidney. A mechanism for renal damage in human monocytic leukaemia.

Mason¹,

Howes²,

Taylor³

et al. 1975

J Clin Pathol

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Purified human lysozyme (muramidase) stimulated potassium excretion by the isolated perfused rat kidney. Lysozyme is filtered and reabsorbed, without a tubular maximum. Over 90% of lysozyme filtered is retained within the kidney; 50% was recovered by enzymic assay and histologically localized to the proximal tubular cells. Hypokalaemia seen in some patients with myelomonocytic leukaemia may be directly attributed to an elevated circulating lysozyme level.

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Section: Muramidasesupporting

confidence: 74%

Effect of human lysozyme (muramidase) on potassium handling by the perfused rat kidney. A mechanism for renal damage in human monocytic leukaemia.

Mason¹,

Howes²,

Taylor³

et al. 1975

J Clin Pathol

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“…Our finding that lysozyme can be degraded by extrarenal mechanisms is a counterpart in the human to the demonstration of Balazs and Roepke (20) in the rat. These workers found linearly rising plasma lysozyme concentrations immediately after bilateral nephrectomy but after 24 hr plasma levels were lower than would be expected on the basis of the initial values.…”

Section: Resultsmentioning

confidence: 73%

“…Turnover data from these curves are shown in Tables IV and V was shown that the increased plasma lysozyme concentration found in severe uremia is due to diminished glomerular filtration whereas the high plasma levels found in certain hematological disorders with disturbed neutrophil turnover is due to increased production of plasma lysozyme probably through release of enzyme from disintegrating cells. The importance of the kidney for the degradation of plasma lysozyme has been shown in both experimental and clinical studies (4,6,(19)(20)(21)(22)(23)(24)(25) although the quantitative relationship between lysozyme turnover and kidney function has not been documented previously.…”

Section: Resultsmentioning

confidence: 99%

Lysozyme turnover in man

Hansen¹,

Karle²,

Andersen³

et al. 1972

J. Clin. Invest.

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A B S T R A C T Lysozyme turnover studies with Ilabeled human lysozyme were carried out on 22 patients, viz. nine control patients, seven nephrological patients with varying degrees of renal insufficiency, including three bilaterally nephrectomized patients, and six hematological patients with disturbed turnover of the neutrophilic granulocytes.It was found that plasma lysozyme has a rapid turnover with a fractional catabolic rate of 76%/hr of the plasma content. Lysozyme catabolism varied with the endogenous creatinine clearance; in addition however, extrarenal sites of catabolism were demonstrated since lysozyme could be broken down in the anephric patients, although only at a rate amounting to about 15% of the rate found in persons with intact kidneys.In the uremic patients the increased plasma lysozyme concentration was due to decreased rates of catabolism; in the hematological patients the increased plasma lysozyme level was due to increased rates of synthesis which supports the hypothesis that plasma lysozyme mainly stems from disintegrating neutrophilic granulocytes. Furthermore, it was shown that in the nonhematological patients examined, the rate of synthesis varied with the endogenous creatinine clearance.

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“…The mean plasma volume of these rats was 4.28% of the body weight (SD = 0.62%); this figure was used in the calculation of the kinetic data in rats 1-5. A comparison of the kinetic data in rats [1][2][3][4][5] with those of rats 6-9 indicated that human albumin apparently did not influence lysozyme turnover.…”

Section: Methodsmentioning

confidence: 97%

“…However, few turnover studies of lysozyme have been carried out, and these were performed either with xenogeneic lysozyme (from hen egg white) or with unphysiological amounts of endogenous lysozyme (1)(2)(3). The aim of the present study was to investigate lysozyme turnover in the rat using radioactively labeled rat lysozyme.…”

Section: Introductionmentioning

confidence: 99%

Lysozyme turnover in the rat

Hansen¹,

Karle²,

Andersen³

1971

J. Clin. Invest.

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A B S T R A C T Lysozyme turnover in the rat was studied with 'I-labeled rat lysozyme. It was found that plasma lysozyme has a rapid disappearance rate with a half-life of 75 min. The rate of synthesis was calculated at 3.4 /Ag/min per 100 g rat. This rate of synthesis was compared with figures from the literature for the turnover rate of neutrophilic granulocytes, and the data were consistent with the concept that disintegrating neutrophils are the main source of plasma lysozyme.The distribution of enzymatic lysozyme activity and of radioactive lysozyme was studied in several organs. Very high enzymatic activity was found in leukocytes as were considerable activities in lungs, kidneys, bone marrow, spleen, and intestine; little enzymatic activity was found in the urine. High radioactive levels as compared with plasma radioactivity were demonstrated only in the kidneys. This indicates that of the organs studied, the kidney is the predominant site of storage and destruction of plasma lysozyme.Lysozyme was found to disappear only slowly from the kidneys over a period of 4 days. The data obtained seem to indicate that lysozyme or a lysozyme degradation product precipitable by trichloroacetic acid was released in small amounts from the kidneys to plasma throughout this period. INTRODUCTIONIncreasing interest in lysozyme (muramidase) as a diagnostic tool in hematology and nephrology has stressed the importance of knowledge of the metabolism of this enzyme. However, few turnover studies of lysozyme have been carried out, and these were performed either with xenogeneic lysozyme (from hen egg white) or with unphysiological amounts of endogenous lysozyme (1-3). The aim of the present study was to investigate lysozyme turnover in the rat using radioactively labeled rat lysozyme.

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Lysozymuria Induced in Rats by Nephrotoxic Agents

Cited by 30 publications

References 2 publications

Effect of human lysozyme (muramidase) on potassium handling by the perfused rat kidney. A mechanism for renal damage in human monocytic leukaemia.

Effect of human lysozyme (muramidase) on potassium handling by the perfused rat kidney. A mechanism for renal damage in human monocytic leukaemia.

Lysozyme turnover in man

Lysozyme turnover in the rat

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