Lysozyme damage caused by secondary degradation products during the autoxidation process of linoleic acid.

Some Analytical Observations of Autoxidation Products of Linoleic Acid and Their Thiobarbituric Acid Reactive Substances

“…The radioactive SP (821 mg) ethanol solution was put in 50 ml of water at pH 9.5 and 150 mg of sodium borohydride was added to the solution. 13 ) This mixture was stood at 37°C for 23 hr. The reduced products were extracted 5 times with diethyl ether.…”

Changes in Lysozyme due to Interaction with Vaporized Hexanal

“…The similarity of our results to those of Matoba et al strongly suggests that aldehydes such as hexanal are major attackers of proteins in oxidizing linoleic acid in the solid state. Thus, the above results of Kanazawa et al 14 ) appear to indicate that the secondary products of oxidizing linoleic acid contain some compounds that only in the aqueous state attack some amino acid residues other than lysine, tryptophan and methionine residues of lysozyme. The arginine residues of lysozyme are impaired by various dicarbonyl compounds.…”

“…Kanazawa et al 14 ) reported that the impaired amino acid residues of lysozyme were tryptophan, lysine, histidine, arginine, glutamic acid, valine, methionine and tyrosine in an aqueous reaction system composed of secondary products of oxidizing linoleic acid. Yanagita and Sugano 15 ) observed that lysine, histidine, arginine, cystine and methionine residues in egg albumin were moderately impaired after storage with ethyl lin oleate at 80.4 %RH.…”

Modificaciones producidas en las proteínas alimentarias por su interacción con lípidos peroxidados. II. Mecanismos conocidos de la interacción lípido (oxidado) - proteína

“…La fuerza del complejo lípido-proteína puede ser definida por una serie de pasos de extracción (12)(13)(14)(15). Kanazawa etal., (15) usando un ácido graso radiactivo, pudieron observar cómo éste era extraído en los pasos consecutivos de extracción.…”

“…Kanazawa etal., (15) usando un ácido graso radiactivo, pudieron observar cómo éste era extraído en los pasos consecutivos de extracción. Si estos complejos físicos son ignorados entonces se pueden malinterpretar los resultados.…”