Lysozyme activity in animal extracts after sodium dodecyl sulfate-polyacrylamide gel electrophoresis

The Detection of Enzyme Activity Following Sodium Dodecyl Sulfate–Polyacrylamide Gel Electrophoresis

“…If the polymer is labeled with a chromogenic, fluorogenic, or radioactive tag, the presence of the enzyme is manifested by the appearance of a zone of clearing visible to the eye, on film, or with a two-dimensional radioactive or luminescent imaging device. For example, dextranase activity can be detected as zones of clearing in a blue background when blue dextran is copolymerized into the gel matrix (134,135), while the clearing of the cloudiness produced when cell wall components are present in the gel matrix permits the ready detection of many autolysins (58,136,137). If the substrate does not possess some intrinsic label or marker, the position of the renatured enzyme may be revealed by staining for the polymer with agents such as Coomassie blue for proteins or ethidium bromide for oligonucleotides.…”

Maternal transfer of humoral specific and non-specific immune parameters to sea bream (Sparus aurata) larvae

“…Electrophoresis was performed as described by Audy et al [40] with slight modifications. Separating (12% acrylamide) gels contained 0.4% (w/v) autoclaved M. lysodeikticus cells (Sigma).…”

Several Thaumatin-Like Proteins Bind to β-1,3-Glucans

“…Laminarin from Laminaria digitata (Peat et al, 1958), ␤-glucan from barley (Hordeum vulgare), chitin, chitosan, lichenan, pustulan, and zymosan from bakers' yeast (Saccharomyces cerevisiae; Di Carlo and Fiore, 1957), thaumatin from Thaumatococcus daniellii, ␤-glucan from Pleurotus ostreatus, and purified Triton X-100 (Audy et al, 1989) were from SigmaAldrich. Laminaritriose, laminaritetraose, laminaripentaose, laminarihexaose, and laminariheptaose were from Seikagaku (PDI BioScience, Aurora, ON, Canada).…”