Several Thaumatin-Like Proteins Bind to β-1,3-Glucans

Trudel, Jean; Grenier, Jean; Potvin, Claude; Asselin, Alain

doi:10.1104/pp.118.4.1431

Cited by 147 publications

(107 citation statements)

References 39 publications

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“…These proteins are typically related to stress responses, such as freezing, osmotic and salt stress and pathogen attack (Chen et al, 1994, Yun et al, 1996, , Tabaeizadeh 1998and Trudel et al, 1998. Thus the water response of maize seems to have characteristics in common to other adverse conditions in agreement with suggestions made for other species (Tabaeizadeh 1998).…”

Section: Discussionsupporting

confidence: 56%

Biochemical and Photosynthetic Evaluation of Responses in Zea mays L. Under Drought Stress

Abu-Muriefah¹,

Ibrahim²,

El-Gaaly³

2014

Curr World Environ

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Antioxidant defense system(s), pigments content and photosynthetic activity as well as some biochemical changes under drought stress were analyzed in maize (Zea mays L. cv. Giza 21) leaves to determine the response of plant to drought stress and to elucidate the role of various protective mechanisms against oxidative stress. It was found that the application of drought stress led to changes in the carbohydrates and protein contents. Total soluble sugars, accumulated in the leaves of water-stressed plants, whereas, starch and protein contents were dropped to a small amounts compared to the control. Furthermore, plants have well-developed defense systems against reactive oxygen species (ROS), involving both limiting the formation of ROS as well as instituting its removal. Within a cell, the activities of a range of antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX) involved in scavenging ROS were investigated. During dehydration the SOD, APX and CAT increased significantly up to 4 days, then declined in their activities but still maintained higher than the control levels this indicates that the defense systems involved are efficient in the protection of plant cells against oxidation. In addition, there was consistent increase in the lipid peroxidation and accumulation of malondialdehyde (MDA). The levels of hydrogen peroxide were also elevated during stressing periods. In this study we are reporting the negative response of maize plants toward drought stress especially on the antioxidant enzymatic activity for the prolonged drought effect.

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Section: Discussionsupporting

confidence: 56%

Biochemical and Photosynthetic Evaluation of Responses in Zea mays L. Under Drought Stress

Abu-Muriefah¹,

Ibrahim²,

El-Gaaly³

2014

Curr World Environ

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“…TL proteins are known as PR-5 proteins in plants, and some are known to exhibit endo-b-1,3-glucanase activity (Trudel et al, 1998). They are considered to exhibit antifungal activity, lysing the b-1,3-glucan in the cell walls of pathogenic fungi.…”

Section: Discussionmentioning

confidence: 99%

“…Activities attributed to these proteins include a sweet taste, antifreeze activity, and antifungal activity (Yun et al, 1998;Datta et al, 1999). In addition, some TL proteins exhibit both b-1,3-glucan binding (Trudel et al, 1998) and endo-b-1,3-glucanase activities (Grenier et al, 1999). Thus, since b-1,3-glucan is a common component of the fungal cell wall, it is possible that plant TL proteins play a role in host defense by destroying the cell walls of pathogenic fungi.…”

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confidence: 99%

Lentinula edodes tlg1 Encodes a Thaumatin-Like Protein That Is Involved in Lentinan Degradation and Fruiting Body Senescence

et al. 2006

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Lentinan is an antitumor product that is purified from fresh Lentinula edodes fruiting bodies. It is a cell wall component, comprising b-1,3-glucan with b-1,6-linked branches, which becomes degraded during postharvest preservation as a result of increased glucanase activity. In this study, we used N-terminal amino acid sequence to isolate tlg1, a gene encoding a thaumatin-like (TL) protein in L. edodes. The cDNA clone was approximately 1.0 kb whereas the genomic sequence was 2.1 kb, and comparison of the two indicated that tlg1 contains 12 introns. The tlg1 gene product (TLG1) was predicted to comprise 240 amino acids, with a molecular mass of 25 kD and isoelectric point value of 3.5. The putative amino acid sequence exhibits approximately 40% identity with plant TL proteins, and a fungal genome database search revealed that these TL proteins are conserved in many fungi including the basidiomycota and ascomycota. Transcription of tlg1 was not detected in vegetative mycelium or young and fresh mushrooms. However, transcription increased following harvest. Western-blot analysis demonstrated a rise in TLG1 levels following harvest and spore diffusion. TLG1 expressed in Escherichia coli and Aspergillus oryzae exhibited b-1,3-glucanase activity and, when purified from the L. edodes fruiting body, demonstrated lentinan degrading activity. Thus, we suggest that TLG1 is involved in lentinan and cell wall degradation during senescence following harvest and spore diffusion.

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“…Several studies have indicated that TLPs react with the cell wall rather than with the membrane. TLPs, including those isolated from barley seed, are able to bind β-1,3-glucan 245,345 and exhibit glucanase (laminarinase) activity 215,366 . Molecular modeling of both barley leaves and seed TLPs highlight the effect of sequence differences between them, in terms of secondary structure and electrostatic potential, suggesting possible implications in target binding specificities of the isoforms 278 .…”

Section: Thaumatin-like Proteins (Tlps) (Pr-5)mentioning

confidence: 99%

A Review: Biological and Technological Functions of Barley Seed Pathogenesis-Related Proteins (PRs)

Gorjanović

2009

Journal of the Institute of Brewing

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The mature barley seed proteome is dominated by proteins involved in stress responses, including the Pathogenesis-Related proteins (PRs). PRs are currently classified into 17 families. The biochemistry of the PRs families, whose members are constitutively present in barley seed, is surveyed in this paper. These proteins are assumed to protect dormant and germinating seeds against pathogenic microorganisms and pests. The current knowledge on PRs structural and functional properties is summarized in an attempt to illustrate their importance in barley seed innate resistance. At the same time, a platform to understand PRs technological function in cereal foods and in beverage processing and quality is provided.

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Several Thaumatin-Like Proteins Bind to β-1,3-Glucans

Cited by 147 publications

References 39 publications

Biochemical and Photosynthetic Evaluation of Responses in Zea mays L. Under Drought Stress

Biochemical and Photosynthetic Evaluation of Responses in Zea mays L. Under Drought Stress

Lentinula edodes tlg1 Encodes a Thaumatin-Like Protein That Is Involved in Lentinan Degradation and Fruiting Body Senescence

A Review: Biological and Technological Functions of Barley Seed Pathogenesis-Related Proteins (PRs)

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