Lysophosphatidylcholine treatment allows interspecies fertilization of hamster oocytes with zona pellucida by human spermatozoa

Riffo, Marta S.; Párraga, Mario

doi:10.1002/jez.1402710211

Cited by 4 publications

(1 citation statement)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…LysoPC, a byproduct of PC hydrolysis, independently modulates ionic gradients and induces depolarization of the sperm membrane (43), thereby enhancing sperm penetration rate and acrosome reaction (44). Additionally, it modifies the zona pellucida and the plasma membrane of both gametes in vitro, promoting gamete fusion (45). LysoPC is closely associated with sperm motility (46)(47)(48), the freeze/thaw cycle of sperm (49), obesity (50), and male infertility (51).…”

Section: Discussionmentioning

confidence: 99%

Lipidomics profiles of human spermatozoa: insights into capacitation and acrosome reaction using UPLC-MS-based approach

Cheng,

Xie,

Xiong

et al. 2023

Front. Endocrinol.

View full text Add to dashboard Cite

IntroductionLipidomics elucidates the roles of lipids in both physiological and pathological processes, intersecting with many diseases and cellular functions. The maintenance of lipid homeostasis, essential for cell health, significantly influences the survival, maturation, and functionality of sperm during fertilization. While capacitation and the acrosome reaction, key processes before fertilization, involve substantial lipidomic alterations, a comprehensive understanding of the changes in human spermatozoa's lipidomic profiles during these processes remains unknown. This study aims to explicate global lipidomic changes during capacitation and the acrosome reaction in human sperm, employing an untargeted lipidomic strategy using ultra-performance liquid chromatography-mass spectrometry (UPLC-MS).MethodsTwelve semen specimens, exceeding the WHO reference values for semen parameters, were collected. After discontinuous density gradient separation, sperm concentration was adjusted to 2 x 106 cells/ml and divided into three groups: uncapacitated, capacitated, and acrosome-reacted. UPLC-MS analysis was performed after lipid extraction from these groups. Spectral peak alignment and statistical analysis, using unsupervised principal component analysis (PCA), bidirectional orthogonal partial least squares discriminant analysis (O2PLS-DA) analysis, and supervised partial least-squares-latent structure discriminate analysis (PLS-DA), were employed to identify the most discriminative lipids.ResultsThe 1176 lipid peaks overlapped across the twelve individuals in the uncapacitated, capacitated, and acrosome-reacted groups: 1180 peaks between the uncapacitated and capacitated groups, 1184 peaks between the uncapacitated and acrosome-reacted groups, and 1178 peaks between the capacitated and acrosome-reacted groups. The count of overlapping peaks varied among individuals, ranging from 739 to 963 across sperm samples. Moreover, 137 lipids had VIP values > 1.0 and twenty-two lipids had VIP > 1.5, based on the O2PLS-DA model. Furthermore, the identified twelve lipids encompassed increases in PI 44:10, LPS 20:4, LPA 20:5, and LPE 20:4, and decreases in 16-phenyl-tetranor-PGE2, PC 40:6, PS 35:4, PA 29:1, 20-carboxy-LTB4, and 2-oxo-4-methylthio-butanoic acid.DiscussionThis study has been the first time to investigate the lipidomics profiles associated with acrosome reaction and capacitation in human sperm, utilizing UPLC-MS in conjunction with multivariate data analysis. These findings corroborate earlier discoveries on lipids during the acrosome reaction and unveil new metabolites. Furthermore, this research highlights the effective utility of UPLC-MS-based lipidomics for exploring diverse physiological states in sperm. This study offers novel insights into lipidomic changes associated with capacitation and the acrosome reaction in human sperm, which are closely related to male reproduction.

show abstract

Section: Discussionmentioning

confidence: 99%

Lipidomics profiles of human spermatozoa: insights into capacitation and acrosome reaction using UPLC-MS-based approach

Cheng,

Xie,

Xiong

et al. 2023

Front. Endocrinol.

View full text Add to dashboard Cite

show abstract

Role of phospholipase A2 in mammalian sperm-egg fusion: Development of hamster oolemma fusibility by lysophosphatidylcholine

Riffo

Párraga

1997

J. Exp. Zool.

View full text Add to dashboard Cite

A postcryogenic comparison of membrane fatty acids of elephant spermatozoa

Swain

Miller

2000

Zoo Biol.

View full text Add to dashboard Cite

Cryogenic protocols have been successful in storing spermatozoa collected from African elephants (Loxodonta africana). However, these same protocols and modifications of these protocols have failed to preserve spermatozoa collected from Asian elephants (Elephas maximus) [Buice et al., 1995, Proceedings of the Association of Zoo Veterinary Technicians, Baltimore, MD; O’Brien et al., 1997, Second International Elephant Research Symposium, Springfield, MO]. Because the success or failure of cryogenic freezing may rely on differences in membrane composition, a postcryogenic comparison of the membrane fatty acid composition of spermatozoa isolated from African and Asian elephants was studied. The spermatozoa of African elephants possessed significantly higher levels of docosahexaenoic acid (22:6, n‐3) and docosapentaenoic acid (22:5, n‐6) as compared to the spermatozoa of Asian elephants. Meanwhile, the spermatozoa of Asian elephants had higher levels of myristic acid (14:0), arachidonic acid (20:4, n‐6), and docosatetraenoic acid (22:4, n‐6) as compared to the spermatozoa of African elephants. The most abundant membrane fatty acid was docosahexaenoic acid (22:6, n‐3). The percentage of membrane docosahexaenoic acid (22:6, n‐3) in the spermatozoa of African elephants was 68.13 ± 0.52 as compared to 42.88 ± 0.87 in the spermatozoa of Asian elephants (t = 31.48, P ≤ 0.0001). Zoo Biol 19:461–473, 2000. © 2000 Wiley‐Liss, Inc.

show abstract

Lysophosphatidylcholine treatment allows interspecies fertilization of hamster oocytes with zona pellucida by human spermatozoa

Cited by 4 publications

References 15 publications

Lipidomics profiles of human spermatozoa: insights into capacitation and acrosome reaction using UPLC-MS-based approach

Lipidomics profiles of human spermatozoa: insights into capacitation and acrosome reaction using UPLC-MS-based approach

Role of phospholipase A2 in mammalian sperm-egg fusion: Development of hamster oolemma fusibility by lysophosphatidylcholine

A postcryogenic comparison of membrane fatty acids of elephant spermatozoa

Contact Info

Product

Resources

About