Role of phospholipase A2 in mammalian sperm-egg fusion: Development of hamster oolemma fusibility by lysophosphatidylcholine

Riffo, Marta S.; Párraga, Mario

doi:10.1002/(sici)1097-010x(19970901)279:1<81::aid-jez8>3.0.co;2-y

Cited by 35 publications

(6 citation statements)

References 22 publications

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“…Supporting a role in fertilization, PLA2 inhibitors did not block the calcium increase in sea urchin fertilization, but did inhibit fertilization envelope formation (an event dependent upon the membrane fusion event of cortical granule exocytosis)(Ajmat et al, 2013). LPC can induce membrane fusion of the sperm plasma membrane and acrosomal membrane (Liguori et al, 2004) as well as human sperm and hamster egg fusion (Riffo and PArraga, 1997). Anti-PLA2 antibodies blocked the interspecies fusion.…”

Section: Lipid Changes During Xenopus Fertilizationmentioning

confidence: 99%

Phospholipase C and D regulation of Src, calcium release and membrane fusion during Xenopus laevis development

Stith

2015

Developmental Biology

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This review emphasizes how lipids regulate membrane fusion and the proteins involved in three developmental stages: oocyte maturation to the fertilizable egg, fertilization and during first cleavage. Decades of work show that phosphatidic acid (PA) releases intracellular calcium, and recent work shows that the lipid can activate Src tyrosine kinase or phospholipase C during Xenopus fertilization. Numerous reports are summarized to show three levels of increase in lipid second messengers inositol 1,4,5-trisphosphate and sn 1,2-diacylglycerol (DAG) during the three different developmental stages. In addition, possible roles for PA, ceramide, lysophosphatidylcholine, plasmalogens, phosphatidylinositol 4-phosphate, phosphatidylinositol 5-phosphate, phosphatidylinositol 4,5-bisphosphate, membrane microdomains (rafts) and phosphatidylinositol 3,4,5-trisphosphate in regulation of membrane fusion (acrosome reaction, sperm-egg fusion, cortical granule exocytosis), inositol 1,4,5-trisphosphate receptors, and calcium release are discussed. The role of six lipases involved in generating putative lipid second messengers during fertilization is also discussed: phospholipase D, autotaxin, lipin1, sphingomyelinase, phospholipase C, and phospholipase A2. More specifically, proteins involved in developmental events and their regulation through lipid binding to SH3, SH4, PH, PX, or C2 protein domains is emphasized. New models are presented for PA activation of Src (through SH3, SH4 and a unique domain), that this may be why the SH2 domain of PLCγ is not required for Xenopus fertilization, PA activation of phospholipase C, a role for PA during the calcium wave after fertilization, and that calcium/calmodulin may be responsible for the loss of Src from rafts after fertilization. Also discussed is that the large DAG increase during fertilization derives from phospholipase D production of PA and lipin dephosphorylation to DAG.

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Section: Lipid Changes During Xenopus Fertilizationmentioning

confidence: 99%

Phospholipase C and D regulation of Src, calcium release and membrane fusion during Xenopus laevis development

Stith

2015

Developmental Biology

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“…Osteopontin is a protein found in the postacrosomal region of cauda epididymal and ejaculates spermatozoa with a role suggested in fertilization and the blocking of the polyspermy [152], and PLA2 is present in the bull sperm plasma membrane [153], and it is related to acrosome reaction events [154], sperm-egg fusion [155, 156], and probable implications in sperm motility [157]. On the other hand, a lower amount of spermadhesin Z13 was found the protein extracts from those bulls, with a variable association of BSP5 expression.…”

Section: Molecular Markers Of Bull Fertilitymentioning

confidence: 99%

Post Testicular Sperm Maturational Changes in the Bull: Important Role of the Epididymosomes and Prostasomes

Caballero

Frenette

Sullivan

2011

Veterinary Medicine International

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After spermatogenesis, testicular spermatozoa are not able to fertilize an oocyte, they must undergo sequential maturational processes. Part of these essential processes occurs during the transit of the spermatozoa through the male reproductive tract. Since the sperm become silent in terms of translation and transcription at the testicular level, all the maturational changes that take place on them are dependent on the interaction of spermatozoa with epididymal and accessory gland fluids. During the last decades, reproductive biotechnologies applied to bovine species have advanced significantly. The knowledge of the bull reproductive physiology is really important for the improvement of these techniques and the development of new ones. This paper focuses on the importance of the sperm interaction with the male reproductive fluids to acquire the fertilizing ability, with special attention to the role of the membranous vesicles present in those fluids and the recent mechanisms of protein acquisition during sperm maturation.

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“…The PLA2 gene expression demonstrated by an in silico analysis of the cumulus cells (Additional file 2 : Table S2) strongly supports that LPC is produced by the cumulus cells. Moreover, LPC at the time of fertilization could be involved in other important steps of fertilization in human as sperm-ZP binding and sperm-oocyte fusion as reported previously in rodents [ 82 , 83 ]. Future experiments are necessary to test this hypothesis.…”

Section: Discussionmentioning

confidence: 65%

Metabolites involved in cellular communication among human cumulus-oocyte-complex and sperm during in vitro fertilization

Gómez‐Torres

García

Guerrero

et al. 2015

Reprod Biol Endocrinol

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BackgroundFertilization is a key physiological process for the preservation of the species. Consequently, different mechanisms affecting the sperm and the oocyte have been developed to ensure a successful fertilization. Thus, sperm acrosome reaction is necessary for the egg coat penetration and sperm-oolema fusion. Several molecules are able to induce the sperm acrosome reaction; however, this process should be produced coordinately in time and in the space to allow the success of fertilization between gametes.The goal of this study was to analyze the metabolites secreted by cumulus-oocyte-complex (COC) to find out new components that could contribute to the induction of the human sperm acrosome reaction and other physiological processes at the time of gamete interaction and fertilization.MethodsFor the metabolomic analysis, eighteen aliquots of medium were used in each group, containing: a) only COC before insemination and after 3 h of incubation; b) COC and capacitated spermatozoa after insemination and incubated for 16–20 hours; c) only capacitated sperm after 16–20 h in culture and d) only fertilization medium as control. Six patients undergoing assisted reproduction whose male partners provided normozoospermic samples were included in the study. Seventy-two COC were inseminated.ResultsThe metabolites identified were monoacylglycerol (MAG), lysophosphatidylcholine (LPC) and phytosphingosine (PHS). Analysis by PCR and in silico of the gene expression strongly suggests that the cumulus cells contribute to the formation of the PHS and LPC.ConclusionsLPC and PHS are secreted by cumulus cells during in vitro fertilization and they could be involved in the induction of human acrosome reaction (AR). The identification of new molecules with a paracrine effect on oocytes, cumulus cells and spermatozoa will provide a better understanding of gamete interaction.Electronic supplementary materialThe online version of this article (doi:10.1186/s12958-015-0118-9) contains supplementary material, which is available to authorized users.

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Role of phospholipase A2 in mammalian sperm-egg fusion: Development of hamster oolemma fusibility by lysophosphatidylcholine

Cited by 35 publications

References 22 publications

Phospholipase C and D regulation of Src, calcium release and membrane fusion during Xenopus laevis development

Phospholipase C and D regulation of Src, calcium release and membrane fusion during Xenopus laevis development

Post Testicular Sperm Maturational Changes in the Bull: Important Role of the Epididymosomes and Prostasomes

Metabolites involved in cellular communication among human cumulus-oocyte-complex and sperm during in vitro fertilization

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