Lysobacter spongiicola sp. nov., isolated from a deep-sea sponge

Abstract

An aerobic, Gram-negative bacterium, strain KMM 329 T , was isolated from a deep-sea sponge specimen from the Philippine Sea and subjected to a polyphasic taxonomic investigation. Comparative 16S rRNA gene sequence analysis showed that strain KMM 329 T clustered with the species of the genus Lysobacter. The highest level of 16S rRNA gene sequence similarity (97.0 %) was found with respect to Lysobacter concretionis KCTC 12205 T ; lower values (96.4-95.2 %) were obtained with respect to the other recognized Lysobacter species. The value for DNA-DNA relatedness between strain KMM 329 T and L. concretionis KCTC 12205 T was 47 %. Branched fatty acids 16 : 0 iso, 15 : 0 iso, 11 : 0 iso 3-OH and 17 : 1 iso were found to be predominant. Strain KMM 329 T had a DNA G+C content of 69.0 mol%. On the basis of the phenotypic, chemotaxonomic, DNA-DNA hybridization and phylogenetic data, strain KMM 329 T represents a novel species of the genus Lysobacter, for which the name Lysobacter spongiicola sp. nov. is proposed. The type strain is KMM 329 T (5NRIC 0728 T 5JCM 14760 T ).

Lysobacter hymeniacidonis sp. nov., isolated from a crude oil-contaminated marine sponge

“…The major cellular fatty acids of strain 2-5 T were iso-C 16: 0 (15.1%), iso-C 15: 0 (14.4%), iso-C 17: 1 ω9c (9.9%), iso-C 11: 0 3-OH (8.4%) and iso-C 11: 0 (7.7%) ( Table 2). The presence of branched fatty acids, namely iso-C 16:0 , iso-C 15: 0 , iso-C 17:1 ω19c and iso-C 17: 0 , was consistent with the placement of strain 2-5 T within the genus Lysobacter (Bae et al, 2005;Weon et al, 2006Weon et al, , 2007Romanenko et al, 2008). However, strain 2-5 T contained C 16:0 ω7c/16:1 ω6c and C 19:0 cyclo ω8c, which differences with the reference strains.…”

Pyrosequencing Reveals Diverse and Distinct Sponge-Specific Microbial Communities in Sponges from a Single Geographical Location in Irish Waters

“…Similar studies have led to the isolation of novel bacterial genera and species from other phyla also. Novel α-Proteobacteria [22], γ-Proteobacteria [8,21,36,37], Bacteroidetes [17][18][19][20] and Verrucomicrobia [39,59] have all been cultured from sponge tissues. There is growing evidence that monophyletic bacterial lineages have co-evolved with their sponge hosts to form sponge-specific clades which are more similar to each other than to similar taxa from non-sponge sources [24,47,54].…”

Lysobacter daecheongensis sp. nov., isolated from sediment of stream near the Daechung dam in South Korea

“…Differential phenotypic characteristics of strain Dae08 T and recognized Lysobacter species Taxa: 1, strain Dae08 T (present study); 2, L. brunescens ATCC 29482 T [data in columns 2, 3, 5, 8, and 14 are from Christensen and Cook (1978) and Weon et al (2007)]; 3, L. gummosus ATCC 29489 T ; 4, L. daejeonensis KACC 11406 T (Weon et al, 2006); 5, L. antibioticus DSM 2044 T ; 6, L. capsici KCTC 22007 T (Park et al, 2008); 7, L. niastensis DSM 18481 T ; 8, L. enzymogenes DSM 2043 T ; 9, L. concretionis DSM 16239 T (Bae et al, 2005); 10, L. spongiicola JCM 14760 T (Romanenko et al, 2007); 11, L. yangpyeongensis KACC 11407 T ; 12, L. koreensis KCTC 12204 T ; 13, L. defluvii DSM 18482 T (Yassin et al, 2007); 14, L. niabensis DSM 18244 T (Weon et al, 2007). +, Positive; (+), weakly positive; -, negative; ND, no data available.…”

“…bacteria with high G+C content, and at present comprises thirteen species with validly published names, namely Lysobacter antibioticus (Christensen and Cook, 1978), Lysobacter brunescens (Christensen and Cook, 1978), Lysobacter capsici (Park et al, 2008), Lysobacter concretionis (Bae et al, 2005), Lysobacter daejeonensis (Weon et al, 2006), Lysobacter defluvii (Yassin et al, 2007), Lysobacter enzymogenes (Christensen and Cook, 1978), Lysobacter gummosus (Christensen and Cook, 1978), Lysobacter koreensis , Lysobacter niabensis (Weon et al, 2007), Lysobacter niastensis (Weon et al, 2007), Lysobacter spongiicola (Romanenko et al, 2007), and Lysobacter yangpyeongensis (Weon et al, 2006).…”