Lysobacter panacisoli sp. nov., isolated from ginseng soil

Abstract

A novel bacterial strain, designated CJ29 T , was isolated from ginseng soil of Anseong in South Korea. Cells of strain CJ29 T were Gram-stain-negative, facultatively anaerobic, rod-shaped and non-motile. Strain CJ29 T grew optimally at 28-30 6C and pH 7.0. Based on 16S rRNA gene sequence analysis, strain CJ29 T was shown to belong to the genus Lysobacter within the class Gammaproteobacteria and was related most closely to Lysobacter soli DCY21 T (98.5 % similarity) and Lysobacter niastensis GH41-7 T (98.2 %). DNA-DNA relatedness between strain CJ29 T and its closest relatives was below 55.6 %. The predominant cellular fatty acids of strain CJ29 T were iso-C 15 : 0 , iso-C 16 : 0 and iso-C 17 : 1 v9c. The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. The major isoprenoid quinone was ubiquinone 8 (Q-8). The G+C content of the genomic DNA was 65.6 mol%. Phenotypic, genotypic and phylogenetic characteristics strongly supported the differentiation of strain CJ29 T from related species of the genus Lysobacter. On the basis of data from this polyphasic taxonomic study, strain CJ29 T is considered to represent a novel species of the genus Lysobacter, for which the name Lysobacter panacisoli sp. nov. is proposed. The type strain is CJ29 T (5KACC 17502 T 5JCM 19212 T ).The genus Lysobacter was first proposed by Christensen & Cook (1978), and classified within the family Xanthomonadaceae belonging to the class Gammaproteobacteria. At the time of writing, the genus Lysobacter comprises 26 species with validly published names (http://www.bacterio.cict.fr/). Species of the genus Lysobacter are commonly found in diverse geographical and environmental habitats, especially in agricultural soil (Liu et al., 2011;Srinivasan et al., 2010;Ten et al., 2009). Members of the genus are generally characterized as Gram-negative, aerobic, non-fruiting and gliding bacteria with a high DNA G+C content typically ranging from 61.7 to 70.1 mol% (Christensen & Cook, 1978;Weon et al., 2006). Species of the genus Lysobacter contain ubiquinone 8 (Q-8) as the major respiratory quinone and show a predominance of iso-branched fatty acids (Fukuda et al., 2013;Luo et al., 2012;Saddler & Bradbury, 2005; Wei et al., 2012). Some members of the genus have potential for development of biocontrol agents based on their ability to lyse a variety of micro-organisms such as Gram-negative and Gram-positive bacteria, filamentous fungi, yeasts, green algae and nematodes (Ahmed et al., 2003;Folman et al., 2004;Kilic-Ekici & Yuen, 2003;Ryazanova et al., 2005). Strain CJ29 T was isolated on R2A agar (BBL) from a soil sample taken from a ginseng field of Anseong in South Korea. The isolate was subsequently cultivated on R2A agar at 30 u C for 3 days and stored as a stock culture in R2A broth (MBCell) supplemented with 30 % (v/v) glycerol. In this study, we describe the morphological, biochemical and phylogenetic characteristics of strain CJ29 T , employing the most closely related type strains, Lysobacter soli KCTC ...

Lysobacter hymeniacidonis sp. nov., isolated from a crude oil-contaminated marine sponge

“…fr/). Recently, three novel species of the genus, Lysobacter arseniciresistens ZS79 T from iron-mined soil (Luo et al, 2012), Lysobacter oligotrophicus 107-E2 T from an Antarctic freshwater lake (Fukuda et al, 2013) and Lysobacter panacisoli CJ29 T from ginsengsoil (Choi et al, 2014) have been described. Members of the genus were of great potential for the development of biocontrol agents against plant fungal pathogens (Islam et al, 2005;Park et al, 2008) and antibiotic bioactivities against human pathogens (Ahmed et al, 2003;Hashizume et al, 2004).…”

Antibacterial activity of aquatic gliding bacteria

“…oligotrophicus , and L . panacisoli have been published (Aslam et al 2009; Choi et al 2014; Fukuda et al 2013; Luo et al 2012; Park et al 2008; Srinivasan et al 2010; Wang et al 2009; Zhang et al 2011). Despite the need for intensive isolation procedures, the screening of these two genera for antimicrobial properties was conducted for the purpose of potential antibiological applications.…”

Lysobacter mobilis sp. nov., isolated from abandoned lead-zinc ore

“…Recently, a member of the genus Lysobacter , Lysobacter thermophilus (Wei et al , 2012), has been reclassified to a new genus as Vulcaniibacterium thermophilum (Yu et al , 2013). At the time of writing, the genus Lysobacter comprised 26 species with validly published names (http://www.bacterio.net/lysobacter.html), including the recently described species Lysobacter panacisoli (Choi et al , 2014). Most members of the genus are able to glide, but are non-motile.…”