Lysinibacillus halotolerans sp. nov., isolated from saline-alkaline soil

Abstract

A novel aerobic, halotolerant bacterium, designated strain LAM612T , was isolated from salinealkaline soil samples from Lingxian County, Shandong Province, China. Cells of strain LAM612T were Gram-reaction-positive, endospore-forming, motile and rod-shaped. The optimal temperature and pH for growth were 35 6C and pH 6.0, respectively. Strain LAM612 T could grow in the presence of up to 10 % (w/v) NaCl. The genomic DNA G+C conten was 36. ranged from 20.6 % to 41.9 %. The major fatty acids of strain LAM612 T were iso-C 15 : 0 (40.8 %), iso-C 16 : 0 (15.2 %) and anteiso-C 15 : 0 (10.8 %). The cell-wall peptidoglycan content was A4a (LLys-D-Asp). The predominant menaquinone was MK-7 and the main polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, three unknown phospholipids, five unknown glycolipids and an unknown lipid. Based on the DNA-DNA hybridization results and phenotypic, phylogenetic and chemotaxonomic properties, strain LAM612 T could be distinguished from the recognized species of the genus Lysinibacillus, and was suggested to represent a novel species of this genus, for which the name Lysinibacillus halotolerans sp. nov. is proposed. The type strain is LAM612 T (5ACCC 00718 T 5JCM 19611 T ).

Lysinibacillus alkaliphilus sp. nov., an extremely alkaliphilic bacterium, and emended description of genus Lysinibacillus

“…S5). The presence of diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol has also been reported for L. halotolerans (Kong et al , 2014) and L. xylanilyticus (Lee et al , 2010). Conversely, strain OMN17 T differs from L. chungkukjangi (Kim et al , 2013) and L. sinduriensis (Jung et al , 2012), by the absence of several unknown phospholipids.…”

“… * Different results compared with published data. † Data from: a , Kong et al (2014); b , Jung et al (2012); c , Kim et al (2013); d , Lee et al (2010). …”

Production of thermostable multiple enzymes from Bacillus amyloliquefaciens KUB29

“…Bacillus. Esses dois gêneros têm sido reportados em diferentes trabalhos que isolaram bactérias do solo (Vilain S et al, 2006;Chikerema SM et al, 2012;Townsley L et al, 2016;Rabha M et al, 2017;Oh H et al, 2017;Kong D et al, 2014;Gallegos-Monterrosa R et al, 2016;Sun JQ, Xu L, Wu XL, 2017).…”

Lysinibacillus acetophenoni sp. nov., a solvent-tolerant bacterium isolated from acetophenone

“…However, some species of the genus Lysinibacillus do not contain phosphatidylethanolamine, but unidentified glycolipids are present as major polar lipids (Jung et al , 2012). To date, there are 21 species (with validly/effectively published names) in the genus Lysinibacillus , with Lysinibacillus varians (Zhu et al , 2014), L. halotolerans (Kong et al , 2014), L. composti (Hayat et al , 2014), ‘ L. fluoroglycofenilyticus ’ (Cheng et al , 2015), L. tabacifolii (Duan et al , 2013), L. chungkukjangi (Kim et al , 2013a), L. contaminans (Kämpfer et al , 2013), ‘ L. jejuensis ’ (Kim et al , 2013b) and L. manganicus (Liu et al , 2013) being recent additions. The names ‘ L. jejuensis ’ and ‘ L. fluoroglycofenilyticus ’ have not yet been validly published.…”