Lysine Succinylation of VBS Contributes to Sclerotia Development and Aflatoxin Biosynthesis in Aspergillus flavus

Wang, Yu; Yang, Mingkun; Ge, Feng; Jiang, Bin; Hu, Rui; Zhou, Xin; Yang, Yunhuang; Liu, Maili

doi:10.1016/j.mcpro.2022.100490

Cited by 9 publications

(15 citation statements)

References 66 publications

(72 reference statements)

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“…In the analysis of function, the Ksuc levels of the immunoprecipitated versicolorin B synthase (VBS) from wild-type [57] VBS and its mutants (K135A and K135R) were compared. It was subsequently validated that the activity of VBS is notably dependent on K135, emphasizing the significance of succinylation at K135 in the maintenance of sclerotia and AF production [58]. In summary, PTMs have vital functions in priming fungal cells for high-stress conditions, particularly during host infection.…”

Section: Post-translational Modifications (Ptms)mentioning

confidence: 95%

“…The succinylation of K47 in S100A10 resulted in the enhancement of the protein's stability by preventing its ubiquitination and subsequent proteasomal degradation via the proteasome [99]. Furthermore, the synthesis of aflatoxin (AF) is inversely associated with the extent of succinylation observed in proteins related to metabolism in the high-aflatoxin strain/low-aflatoxin strain (H-AFs/L-AFs) [58]. Intriguingly, Ren et al observed that Aspergillus flavus, when exposed to sodium succinate, displayed increased succinylation of total proteins but experienced a substantial reduction in aflatoxin production [56].…”

Section: Enzymatic Lysine Succinylationmentioning

confidence: 99%

“…Subsequently, Fas-1 and Fas-2-driven catalysis is responsible for creating the initial hexanoate unit [101]. Wang et al [58] notably observed the downregulation of succinylation in acetyl-CoA carboxylase, Fas-1, and Fas-2 in H-AFs. These findings imply that Ksuc plays a significant role as a post-translational modification in the secondary metabolic pathway of AF synthesis.…”

Section: Enzymatic Lysine Succinylationmentioning

confidence: 99%

“…These findings imply that Ksuc plays a significant role as a post-translational modification in the secondary metabolic pathway of AF synthesis. It potentially exerts direct control over the structure or functionality of the enzymes responsible for the initial phase of AF synthesis [58].…”

Section: Enzymatic Lysine Succinylationmentioning

confidence: 99%

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Impact of Lysine Succinylation on the Biology of Fungi

Adejor,

Tumukunde,

et al. 2024

CIMB

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Post-translational modifications (PTMs) play a crucial role in protein functionality and the control of various cellular processes and secondary metabolites (SMs) in fungi. Lysine succinylation (Ksuc) is an emerging protein PTM characterized by the addition of a succinyl group to a lysine residue, which induces substantial alteration in the chemical and structural properties of the affected protein. This chemical alteration is reversible, dynamic in nature, and evolutionarily conserved. Recent investigations of numerous proteins that undergo significant succinylation have underscored the potential significance of Ksuc in various biological processes, encompassing normal physiological functions and the development of certain pathological processes and metabolites. This review aims to elucidate the molecular mechanisms underlying Ksuc and its diverse functions in fungi. Both conventional investigation techniques and predictive tools for identifying Ksuc sites were also considered. A more profound comprehension of Ksuc and its impact on the biology of fungi have the potential to unveil new insights into post-translational modification and may pave the way for innovative approaches that can be applied across various clinical contexts in the management of mycotoxins.

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Section: Post-translational Modifications (Ptms)mentioning

confidence: 95%

Section: Enzymatic Lysine Succinylationmentioning

confidence: 99%

Section: Enzymatic Lysine Succinylationmentioning

confidence: 99%

Section: Enzymatic Lysine Succinylationmentioning

confidence: 99%

See 2 more Smart Citations

Impact of Lysine Succinylation on the Biology of Fungi

Adejor,

Tumukunde,

et al. 2024

CIMB

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“…Besides the biosynthetic pathway and its internal gene regulation, protein post-translational modifications (PTMs), an important mean of epigenetics, represent an important role in the regulation of aflatoxins synthesis, including 2-hydroxyisobutyrylation, succinylation, acetylation, and methylation [18][19][20][21][22][23][24] , in which Snt2 (also called sntB, an epigenetic reader) is deeply involved. Despite advancements in the field, our understanding of the molecular mechanisms of aflatoxin production in A. flavus is still fragmentary.…”

Section: Introductionmentioning

confidence: 99%

SntB triggers the antioxidant pathways to regulate development and aflatoxin biosynthesis inAspergillus flavus

Wu,

Yang,

Yao

et al. 2023

Preprint

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The epigenetic reader SntB was identified as an important transcriptional regulator of growth, development, and secondary metabolite synthesis inAspergillus flavus. However, the underlying molecular mechanism is still unclear. In this study,sntBgene deletion (ΔsntB), complementary (Com-sntB), and HA tag fused tosnt2(snt2-HA) strains were constructed by using the homologous recombination method, respectively. Our results revealed that deletion ofsntBinhibited the processes of mycelia growth, conidial production, sclerotia formation, aflatoxin synthesis, and ability to colonize host compared to wild type (WT), and the defective phenotype of knockout strain ΔsntBcan be restored by its complementary strain Com-sntB. Chromatin immunoprecipitation sequencing (ChIP-seq) ofsntB-HA and WT and RNA sequencing (RNA-seq) of ΔsntBand WT strains revealed that SntB played key roles in oxidative stress response ofA. flavus. The function ofcatC(encode a catalase) gene was further analyzed based on the integration results of ChIP-seq and RNA-seq. In ΔsntBstrain, the relative expression level ofcatCwas significantly higher than in WT strain, while a secretory lipase encoding gene (G4B84_008359) was down-regulated. Under the stress of oxidant menadione sodium bisulfite (MSB), the deletion ofsntBobvious down-regulated the expression level ofcatC. After deletion ofcatCgene, the mycelia growth, conidial production, and sclerotia formation were inhibited, while aflatoxin synthesis was increased compared to the WT strain. Results also showed that the inhibition rate of MSB to ΔcatCstrain was significantly lower than that of WT group and AFB1 yield of the ΔcatCstrain was significantly decreased than that of WT strain under the stress of MSB. Our study revealed the potential machinery that SntB regulated fungal morphogenesis, mycotoxin anabolism, and fungal virulence through the axle of from SntB to fungal virulence and mycotoxin bio-synthesis, i.e. H3K36me3 modification-SntB-Peroxisomes-Lipid hydrolysis-fungal virulence and mycotoxin bio-synthesis. The results of the study shad light into the SntB mediated epigenetic regulation pathway of fungal mycotoxin anabolism and virulence, which provided potential strategy for control the contamination ofA. flavusand its aflatoxins.

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