Comprehensive analysis of aflatoxin B1 biosynthesis in Aspergillus flavus via transcriptome-wide m6A methylome response to cycloleucine

Liang, Liuke; Wang, Xiaoyan; Lan, Haier; Wei, Shan; Lei, Yang; Zhang, Shuaibing; Zhai, Huanchen; Hu, Yuansen; Lv, Yangyong

doi:10.1016/j.jhazmat.2023.132677

Cited by 6 publications

(2 citation statements)

References 67 publications

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“…Food safety is currently a crucial global issue. According to statistics from the Food and Agriculture Organization of the United Nations, approximately 25% of worldwide food is annually contaminated by Aspergillus flavus and aflatoxins (AFs). , A. flavus is a notorious fungus that parasitizes food and produces secondary metabolites, including highly carcinogenic AFs, posing a serious threat to human and animal health.…”

Section: Introductionmentioning

confidence: 99%

“…According to statistics from the Food and Agriculture Organization of the United Nations, approximately 25% of worldwide food is annually contaminated by Aspergillus flavus and aflatoxins (AFs). 1,2 A. flavus is a notorious fungus that parasitizes food and produces secondary metabolites, including highly carcinogenic AFs, posing a serious threat to human and animal health. It primarily contaminates crops, such as corn, peanuts, and nuts, causing enormous losses in agricultural economy and compromising global agricultural production and storage processes.…”

Section: ■ Introductionmentioning

confidence: 99%

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The Development of Aspergillus flavus and Biosynthesis of Aflatoxin B1 are Regulated by the Golgi-Localized Mn²⁺ Transporter Pmr1

Qu,

Chi,

2024

J. Agric. Food Chem.

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Microorganisms rely on diverse ion transport and trace elements to sustain growth, development, and secondary metabolism. Manganese (Mn 2+ ) is essential for various biological processes and plays a crucial role in the metabolism of human cells, plants, and yeast. In Aspergillus flavus, we confirmed that Pmr1 localized in cis-and medial-Golgi compartments was critical in facilitating Mn 2+ transport, fungal growth, development, secondary metabolism, and glycosylation. In comparison to the wild type, the Δpmr1 mutant displayed heightened sensitivity to environmental stress, accompanied by inhibited synthesis of aflatoxin B1, kojic acid, and a substantial reduction in pathogenicity toward peanuts and maize. Interestingly, the addition of exogenous Mn 2+ effectively rectified the developmental and secondary metabolic defects in the Δpmr1 mutant. However, Mn 2+ supplement failed to restore the growth and development of the Δpmr1Δgdt1 double mutant, which indicated that the Gdt1 compensated for the functional deficiency of pmr1. In addition, our results showed that pmr1 knockout leads to an upregulation of O-glycosyl-Nacetylglucose (O-GlcNAc) and O-GlcNAc transferase (OGT), while Mn 2+ supplementation can restore the glycosylation in A. flavus. Collectively, this study indicates that the pmr1 regulates Mn 2+ via Golgi and maintains growth and metabolism functions of A. flavus through regulation of the glycosylation.

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Section: Introductionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

The Development of Aspergillus flavus and Biosynthesis of Aflatoxin B1 are Regulated by the Golgi-Localized Mn²⁺ Transporter Pmr1

Qu,

Chi,

2024

J. Agric. Food Chem.

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Insight into the regulatory mechanism of m6A modification: From MAFLD to hepatocellular carcinoma

Zha,

Gao,

et al. 2024

Biomedicine & Pharmacotherapy

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Colorimetric and ECL dual-mode aptasensor for smartphone-based onsite sensitive detection of aflatoxin B1 in combination with ZnO@MWCNTs/g-C3N4 nanosheets and CuO@CuPt nanocomposites

Xu,

Li,

Mao

et al. 2024

Biosensors and Bioelectronics

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Comprehensive analysis of aflatoxin B1 biosynthesis in Aspergillus flavus via transcriptome-wide m6A methylome response to cycloleucine

Cited by 6 publications

References 67 publications

The Development of Aspergillus flavus and Biosynthesis of Aflatoxin B1 are Regulated by the Golgi-Localized Mn²⁺ Transporter Pmr1

The Development of Aspergillus flavus and Biosynthesis of Aflatoxin B1 are Regulated by the Golgi-Localized Mn²⁺ Transporter Pmr1

Insight into the regulatory mechanism of m6A modification: From MAFLD to hepatocellular carcinoma

Colorimetric and ECL dual-mode aptasensor for smartphone-based onsite sensitive detection of aflatoxin B1 in combination with ZnO@MWCNTs/g-C3N4 nanosheets and CuO@CuPt nanocomposites

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Comprehensive analysis of aflatoxin B1 biosynthesis in Aspergillus flavus via transcriptome-wide m6A methylome response to cycloleucine

Cited by 6 publications

References 67 publications

The Development of Aspergillus flavus and Biosynthesis of Aflatoxin B1 are Regulated by the Golgi-Localized Mn2+ Transporter Pmr1

The Development of Aspergillus flavus and Biosynthesis of Aflatoxin B1 are Regulated by the Golgi-Localized Mn2+ Transporter Pmr1

Insight into the regulatory mechanism of m6A modification: From MAFLD to hepatocellular carcinoma

Colorimetric and ECL dual-mode aptasensor for smartphone-based onsite sensitive detection of aflatoxin B1 in combination with ZnO@MWCNTs/g-C3N4 nanosheets and CuO@CuPt nanocomposites

Contact Info

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The Development of Aspergillus flavus and Biosynthesis of Aflatoxin B1 are Regulated by the Golgi-Localized Mn²⁺ Transporter Pmr1

The Development of Aspergillus flavus and Biosynthesis of Aflatoxin B1 are Regulated by the Golgi-Localized Mn²⁺ Transporter Pmr1