Lymphoglandular complexes of the normal colon: Histochemistry and immunohistochemistry

O'Leary, Allison; Sweeney, E. C.

doi:10.1007/bf02953232

Cited by 15 publications

(9 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…6). Similarly, O´Leary & Sweeny (1987) reported in humans that mucin produced by LGC epithelium showed a gradual reduction in proportion of neutral and increase in proportion of acidic mucopolysaccharides. Glandular diverticulum into lymphoid tissue of proximal colon patch showed strong (+++) positive reaction for mucin by Mayer mucicarmine method.…”

Section: Resultsmentioning

confidence: 60%

Lymphoglandular Complexes in Proximal Colon of Buffalo Calves (Bubalus bubalis)

Kapoor¹,

Singh²

2016

Int. J. Morphol.

View full text Add to dashboard Cite

SUMMARY:The present study was conducted on six healthy early neonatal and six prepubertal buffalo calves to study the location, gross morphology, histomorphology and histochemistry of lymphoglandular complexes in proximal colon. In very proximal part of colon of buffalo calves, an irregular oval mucosal lymphoid patch was found grossly as a proximal colon (PC) patch. Histologically, in proximal colon patch of early neonates (3-4 weeks), an extensive invasion of mucosal glands was observed towards lymphoid nodules that were present in submucosa. The structure as a whole thus formed a complex known as lymphoglandular complex (LGC). Large number of such complexes i.e., LGCs were observed in submucosa of proximal colon at this age. At some places, invasion of mucosal glands into lymphoid tissue was restricted to superficial layer of complexes, with the lymphoglandular complexes opening directly into the lumen but some were deep seated. However, by the age of 6 months in buffalo calves i.e., prepubertal period, LGCs were reduced and were present in single layer within the submucosa of the proximal colon. Moreover, some of LGCs were completely encapsulated by their own lamina muscularis mucosae. But some of the complexes still had their mucosal openings into lumen while others had lost their connection with tunica mucosa. Histochemically, the glands that were observed within LGCs contained mucosubstances, glycogen, mucopolysaccharides, and mucin. However, lipids were present around the lymphocytes observed towards the periphery of these LGCs.

show abstract

Section: Resultsmentioning

confidence: 60%

Lymphoglandular Complexes in Proximal Colon of Buffalo Calves (Bubalus bubalis)

Kapoor¹,

Singh²

2016

Int. J. Morphol.

View full text Add to dashboard Cite

show abstract

“…A battery of recently developed monoclonal antibodies was used to localize and quantify specific lymphocyte subsets within the LGC by immunohistochemistry and flow cytometry, respectively. Results confirm similarity between the colonic LGCs of pigs and humans (117) and cattle (134). The LGC of pigs is more similar to persistent GALT of the small intestine (discrete or jejunal Peyer's patches) than to the transient ileal patch with features of primary lymphoid tissue (17), seen also in lambs (147) and calves (85), or to proximal colonic lymphoid nodules of rodents (138,474).…”

Section: General Discussion and Summarysupporting

confidence: 61%

“…GALT is very limited; one report each is available for humans, mice, cattle, and rats (45,117,134,138). In human lymphoglandular complexes (LGCs) 117 Lymphocytes of the proximal colonic lymphoid tissue in mice and rats differ from those in other GALT in that they are steroid sensitive, suggesting that they are immature (45,138).…”

Section: Analysis Of Lymphocellular Composition and Localization For mentioning

confidence: 99%

“…GALT is very limited; one report each is available for humans, mice, cattle, and rats (45,117,134,138). In human LGCs 117 LGCs persist into adulthood (personal observation).…”

Section: Analysis Of Lymphocellular Composition and Localization For mentioning

confidence: 99%

“…Retterer [1892], cited in 150), humans(49,75,78,87,116,117), Australian marsupial mice(144), mice(122,138), pigs(18,50,54,61,74,80,107,109,154), rats(20,45), sheep(61), and subhuman primates(Orth [1901], cited in 54).…”

mentioning

confidence: 99%

See 2 more Smart Citations

Role of the colonic lymphoglandular complex in mucosal immunity in swine

Morfitt

View full text Add to dashboard Cite

Ultrastructural and cytoarchitectural features of lymphoreticular organs in the colon and rectum of adult BALB/c mice

1991

View full text Add to dashboard Cite

The structure and function of colonic mucosal lymphoid organs remain largely unexplored, especially in the rectum hidden within the pelvic vault. Two-month-old female BALB/c mice were anesthetized, and the entire colon was removed from cecum to anus. Distal colonic patches were then prepared for electron microscopy or were quick-frozen and sectioned for immunoperoxidase localization of B cells and T cell subsets. Aggregated lymphoid follicles were distributed irregularly along the entire colon with an average of 1.4 patches per centimeter of colon length. There were large collections of follicles opposite the ileocecal valve (cecal patches), variable numbers of patches throughout the colon, and at least one patch within 10 mm of the anus (rectal patch). Follicles were adjacent to branching crypts lined by epithelium infiltrated by lymphoid cells and containing few goblet cells. In electron micrographs, M cells were identified by their short, irregular microvilli; intraepithelial lymphoid cells; reduced lysosomal dense bodies; and an expanded tubulovesicular network. Small germinal centers were seen. Cytoarchitectural components of colonic lymphoid follicles and Peyer's patch follicles were remarkably similar, despite differences in surrounding mucosa and luminal microbial exposure. The presence of organized lymphoid tissue with M cells and germinal centers suggests that transepithelial particle transport and antigen recognition can take place in the rectum. Whether such tissue has the capacity for uptake of luminal microorganisms is of particular interest, not only because colonic follicles may be sites for local initiation of immune responses but also because they may be important entry points for systemic infection.

show abstract

Lymphoglandular complexes of the normal colon: Histochemistry and immunohistochemistry

Cited by 15 publications

References 14 publications

Lymphoglandular Complexes in Proximal Colon of Buffalo Calves (Bubalus bubalis)

Lymphoglandular Complexes in Proximal Colon of Buffalo Calves (Bubalus bubalis)

Role of the colonic lymphoglandular complex in mucosal immunity in swine

Ultrastructural and cytoarchitectural features of lymphoreticular organs in the colon and rectum of adult BALB/c mice

Contact Info

Product

Resources

About