Lymphocytes modulated by β interferon express new non-HLA-A,B,C class I antigens

Gazit, Ephraim; Gil, R.; Altshuler, R.

doi:10.1016/0198-8859(87)90112-1

Cited by 9 publications

(4 citation statements)

References 22 publications

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“…Mutant .184 TGr3 was derived from . 45 and is a hypoxanthine/guanine phosphoribosyltransferase-negative (HPRT-) derivative (10) of .184, which has homozygous deletions of the HLA-A and -B loci but still expresses the maternal HLA-C allele; TGr refers to thioguanine resistance (20). Details of the derivation and properties of HLA-A,B,C-null mutant .221 will be described elsewhere.…”

mentioning

confidence: 99%

Transfer and expression of three cloned human non-HLA-A,B,C class I major histocompatibility complex genes in mutant lymphoblastoid cells.

Shimizu

Geraghty

Koller

et al. 1988

Proc. Natl. Acad. Sci. U.S.A.

194

126

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The HLA-A, -B, and -C class I human histocompatibility antigens and the genes that encode them have been isolated and characterized. Apparently complete class I non-HLA-A,B,C genes have been identified on HindU-generated 5.4-kilobase (kb), 6.0-kb, and 6.2-kb DNA fragments derived from lymphoblastoid cell line (LCL) 721. We studied the expressibility of these genes by subcloning them into the nonintegrating pHeBo vector and transferring the chimeric plasmids into mutant LCL 721. lysates showed that transfer of each non-HL4-AB,C class I gene into 721.221 resulted in the appearance of an a chain that coprecipitated with P2-microglobulin. The three previously unreported a chains differed from each other in size and were smaller than HLA-A, -B, and -C a chains. These observations clearly show that these three cloned, nonallelic, non-HLA-A,B,C class I genes encode a chains that can be expressed in human cells.

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mentioning

confidence: 99%

Transfer and expression of three cloned human non-HLA-A,B,C class I major histocompatibility complex genes in mutant lymphoblastoid cells.

Shimizu

Geraghty

Koller

et al. 1988

Proc. Natl. Acad. Sci. U.S.A.

194

126

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“…During this workshop, there were several discussions about the expression of epitopes on the cell surface, not only on normal cells but also on leukemic cells. Several publications mention an increased expression of the antigens described in this workshop (Mitsuishi et al 1986, van Leeuwen et al 1986, Gazit et al 1987). Futhermore, alpha and beta interferon can influence the expression on PHA blasts, while gamma interferon is able to increase the expression on TF lymphocytes (Gazit et al 1987, van Leeuwen, unpublished observation).…”

Section: Clusters Of Serum Results Of Anti-tca Sera From Leiden and Amentioning

confidence: 92%

The Leiden “Class IV” or “Class I‐like” workshop November 20–23, 1986

Leeuwen¹,

D'Amaro²,

Fauchet³

et al. 1988

Tissue Antigens

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“…These sequences have in some ea.ses been mapped distal to the HLA-C region, and it has been hypothesized on ihe basis of the murine system that any potential Tl/Qa homologues would indeed be located in this region [29]. A series of polymorphic /^^m-associated antigens termed HT have been described, primarily expressed on lymphocytes stimulated by /f-interferon and the T-cell mitogen PHA, some of which were found to segregate with alleles of the HLA-A locus [20,21]. A similar system of alloantigens detectable on subpopulations of peripheral blood T-cells have also been reported, one of whieh (TCA) was found to be ^;m-associated and to show limited segregation with HLA, possibly encoded by gene sequences located telometric to HLA-A [19].…”

Section: Discussionmentioning

confidence: 99%

“…Sufficient biochemical, immunotogical and immunogenetic differences have been reported between murine and human antigens to seriously undermine this concept however [14][15][16][17], and it is now recognized that CDl molecules are not encoded in the MHC region on chromosome six [ 18]. Further potential candidates for the tnantle of Qa-like antigens arc the HLA-linked TCA [19] and HT [20,21] moiccuics. Having previously reported the expression of CD I c on malignant B cells at defined stages of differentiation [22].…”

mentioning

confidence: 99%

The MHC Class I Associated Beta₂‐microglobulin (β₂m) Light Chain is Expressed in a Molar Excess Over HLA‐ABC and CD1 on the Membrane of Leukaemic B Cells but not Leukaemic T Cells: Evidence for Further β₂m‐Associated Molecules

Jones¹,

Child²,

Master³

et al. 1991

Scand J Immunol

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Beta 2-microglobulin (beta 2m) constitutes the common light chain of both the MHC-encoded HLA-ABC molecules and a group of structurally related glycoproteins recognized by antibodies of the first cluster of differentiation (CD1a, CD1b and CD1c). These CD1 antigens appear similar to murine T1 and Qa molecules in terms of structure and tissue distribution, although the question of inter-species homology is controversial. A further group of alloantigens expressed predominantly on T cells has been reported however, with immunogenetic characteristics more closely analogous to the murine T1/Qa system than the CD1 antigens, although their precise identity remains ill-defined. Having previously shown that malignant B cells may express membrane CD1c, we examined leukaemic B-cells corresponding to early lymphoblastic differentiation (null- and common acute lymphoblastic leukaemia) through to the terminal plasma cell stage for the expression of other non-HLA class I beta 2m-associated molecules. It was found that leukaemic B-cells at intermediate/late stages of differentiation, represented by non-Hodgkin's lymphoma (B-NHL) and 'hairy-cell' leukaemia (HCL), had significantly higher beta 2m:HLA-ABC ratios than did the cells from other types of B-cell malignancy. Although leukaemic B cells with a demonstrable non HLA-ABC-associated beta 2m component expressed detectable levels of CD1c, and insignificant levels of CD1a and CD1b, the antigen density was insufficient to account for the excess beta 2m. In vitro stimulation of leukaemic B cells by phorbol ester substantially increased the expression of HLA-ABC and CD1c, but also accentuated further the difference between the expression of these molecules and that of beta 2m. There was no detectable beta 2m other than that associated with HLA-ABC and CD1 on the surface of malignant T cells by contrast. Our findings strongly support the existence, at certain stages of leukaemic B-cell differentiation, of an additional beta 2m component(s) other than that associated with HLA-ABC and CD1.

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Lymphocytes modulated by β interferon express new non-HLA-A,B,C class I antigens

Cited by 9 publications

References 22 publications

Transfer and expression of three cloned human non-HLA-A,B,C class I major histocompatibility complex genes in mutant lymphoblastoid cells.

Transfer and expression of three cloned human non-HLA-A,B,C class I major histocompatibility complex genes in mutant lymphoblastoid cells.

The Leiden “Class IV” or “Class I‐like” workshop November 20–23, 1986

The MHC Class I Associated Beta₂‐microglobulin (β₂m) Light Chain is Expressed in a Molar Excess Over HLA‐ABC and CD1 on the Membrane of Leukaemic B Cells but not Leukaemic T Cells: Evidence for Further β₂m‐Associated Molecules

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Lymphocytes modulated by β interferon express new non-HLA-A,B,C class I antigens

Cited by 9 publications

References 22 publications

Transfer and expression of three cloned human non-HLA-A,B,C class I major histocompatibility complex genes in mutant lymphoblastoid cells.

Transfer and expression of three cloned human non-HLA-A,B,C class I major histocompatibility complex genes in mutant lymphoblastoid cells.

The Leiden “Class IV” or “Class I‐like” workshop November 20–23, 1986

The MHC Class I Associated Beta2‐microglobulin (β2m) Light Chain is Expressed in a Molar Excess Over HLA‐ABC and CD1 on the Membrane of Leukaemic B Cells but not Leukaemic T Cells: Evidence for Further β2m‐Associated Molecules

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The MHC Class I Associated Beta₂‐microglobulin (β₂m) Light Chain is Expressed in a Molar Excess Over HLA‐ABC and CD1 on the Membrane of Leukaemic B Cells but not Leukaemic T Cells: Evidence for Further β₂m‐Associated Molecules