The Leiden “Class IV” or “Class I‐like” workshop November 20–23, 1986

We have used the human teratocarcinoma-derived embryonal carcinoma cell line Tera-2 cl. 13 to explore the putative expression of novel HLA class I(-like) genes. Serological analyses revealed that Tera-2 cells do not express polymorphic HLA class I (-A, -B, -C) specificities, but do express HLA class I-like antigens. These phenotypic properties parallel those of certain mouse embryonal carcinoma cells. To study the expression of HLA class I(-like) genes in the Tera-2 cells two different approaches were used. Screening of a Tera-2 cDNA library with a full-length HLA class I cDNA probe under conditions that would allow for the identification of relatively distinct HLA class I-like sequences yielded 27 positive clones, all of which were of the regular HLA-A, -B, -C type. Reverse northern hybridizations of the restriction enzyme-digested Tlab region comprising cosmids with Tera-2 cDNA as the probe resulted in the identification of several putative human genes whose equivalents map within the mouse Tla region. However, none of these genes appeared to be structurally related to HLA class I. A putative H3.3 histone gene was identified in the proximal Tla region of the C57BL/10 mouse. It is concluded that no structural homologues of mouse Qa/Tla genes are expressed in the human developmental cell line Tera-2.

show abstract

HLA‐A incompatibility associated with enhanced long‐term renal graft survival in HLA‐B, DR mismatched transplants

Bućin¹,

Ekberg²,

Lindholm³

et al. 1994

Immunology & Cell Biology

View full text Add to dashboard Cite

SummaryThe effect of HLA-A matching on long-term cadaver kidney graft survival was analysed, on average, 6 years after transplantation in a total of 1085 cyclosporine (CyA)-treated patients.A beneficial effect of HLA-A mismatching on graft survival was found by univariate and multivariate analyses (?< 0.05).Enhanced graft survival was associated with HLA-A mismatching in transplants mismatched for HLA-B,DR (/*= 0.03), but not in HLA-B,DR compatible transplants.High 6 year graft survival rates, 78% and 66%. were found in transplants mismatched for two or one HLA-A antigens, respectively, among patients without any acute rejection episode. This was significantly higher than the survival rate of 55% found in HLA-A compatible transplants iP = 0.00\). In patients who had suffered from acute rejection episodes, a prolonged graft survival was also associated with HLA-A mismatching in HLA-B.DR mismatched transplants {P-0.04).The beneficial effect on graft survival of HLA-A mismatching was most pronounced in patients treated with high/medium dose CyA and prednisolone {P = 0.004 overall and P = 0.0007 for HLA-B,DR mismatched transplants).In conclusion. HLA-A mismatching was associated with enhanced long-term renal graft survival in CyA-treated recipients of HLA-B,DR mismatched transplants. In clinical situations, the present results might, if confirmed, contribute to the prolongation of long-term graft survival. The results might indicate the existence of tolerance promoting allogeneic markers within the HLA-A class I region.

show abstract

The Leiden “Class IV” or “Class I‐like” workshop November 20–23, 1986

Cited by 3 publications

References 20 publications

Antigen Society #31 Report Part 2: Antigen Society #31 Report

Antigen Society #31 Report Part 2: Antigen Society #31 Report

HLA class I homologous transcripts in the human embryonal carcinoma cell line Tera-2

HLA‐A incompatibility associated with enhanced long‐term renal graft survival in HLA‐B, DR mismatched transplants

Contact Info

Product

Resources

About