Lymphocytes bearing the T cell receptor gamma delta in human breast milk.

Bertotto, A; Castellucci, G; Fabietti, G.; Scalise, F; Vaccaro, R

doi:10.1136/adc.65.11.1274-a

Cited by 37 publications

(13 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A more likely origin of HIV-1 in breast milk is infected cells from gut-associated lymphoid tissue (GALT) [16, 51, 52]. Cellular markers indicate that T cells in human breast milk migrate from tissues other than the PBMC [53-55], while both rodent and human studies show that some lymphocytes in breast milk originate from the gut [9-13]. The predominant lymphocytes in the breast milk are CCR5-expressing memory CD4 T-cells [55], although macrophages are also a major cell type in breast milk [56], and a likely source in breast milk of HTLV-1 infection [57].…”

Section: Discussionmentioning

confidence: 99%

Multiple independent lineages of HIV-1 persist in breast milk and plasma

Gray

Salemi

Lowe³

et al. 2011

Aids

View full text Add to dashboard Cite

Design-The origin and evolution of HIV-1 in breast milk is unclear, despite the continuing significance of this tissue as a transmitting compartment. To elucidate the evolutionary trajectory of viral populations in a transient mucosal compartment, longitudinal sequences of the envelope gp120 region from plasma and breast milk spanning the first year after delivery were analyzed in six women infected by HIV-1 subtype C.Methods-Multiple phylogenetic algorithms were used to elucidate the evolutionary history and spatial structure of virus populations between tissues.Results-Overall persistent mixing of viral sequences between plasma and breast milk indicated that breast milk is not a distinct genetic viral compartment. Unexpectedly, longitudinal phylogenies showed multiple lineages defined by long branches that included virus from both the breast milk and the plasma. Plasma was unlikely the anatomical origin of the most recent common ancestor (MRCA) in at least three of the subjects, while in other women, the temporal origin of the MRCA of the viral populations following delivery occurred well before the onset of breast milk production.Corresponding Author: Maureen M. Goodenow 2033 Mowry Road PO Box 103633 Gainesville, Fl, 32610-3633. * These authors contributed equally to the manuscript. This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. Conclusions-These findings suggest that during pregnancy/lactation, a viral variant distinct from the plasma virus initially seeds the breast milk, followed by subsequent gene flow between the plasma and breast milk tissues. This study indicates the potential for reactivation or reintroduction of distinct lineages during major immunological disruptions during the course of natural infection. NIH Public Access

show abstract

Section: Discussionmentioning

confidence: 99%

Multiple independent lineages of HIV-1 persist in breast milk and plasma

Gray

Salemi

Lowe³

et al. 2011

Aids

View full text Add to dashboard Cite

show abstract

“…According to Wirt et al [12], 83% of the lymphocytes in human milk are T cells as shown by the presence of CDy. Lym phocytes bearing gamma-delta T-cell recep tors in a proportion twofold greater than in peripheral blood have been found by Bertotto et al [13]. In another study, Bertotto et al [14] reach the conclusion that milk lympho cytes comprise those types which exhibit CD45Rlow, CDw29, UCHL1, and LFA-1 high-memory T-cell phenotype.…”

Section: Discussionmentioning

confidence: 64%

Phagocytosis-Promoting Factor in Human Colostrum

Straussberg

Sirota²,

Hart

et al. 1995

Neonatology

View full text Add to dashboard Cite

The effect of colostrum of mothers of preterm and full-term newborns on the phagocytic activity for latex particles by normal peripheral blood polymorphonuclear cells was examined. The results showed that human colostrum contains a phagocytosis-promoting factor(s), which not only increases the number of phagocytic cells, but also stimulates the phagocytic activity of the individual cell. This factor(s) was more active in preterm colostrum. Thus, while term colostrum increased the number of phagocytic cells by 95% in comparison with the control, preterm colostrum enhanced the number of phagocytic cells by 300%. Similarly, term colostrum increased the phagocytic capacity of the individual cell by 51%, whereas preterm colostrum by 137%. The difference between the results in all experimental points was statistically highly significant.

show abstract

“…Combined with their ability to secrete a variety of lymphokines, there is currently substantial interest in their role(s) in human milk. The majority of lymphocytes in human milk are T cells (83%), including both helper-inducer (CD3+/CD4+) and suppressor/ cytotoxic (CD3+/CD8+) phenotypes with a small number of B cells (4-92 W. L. DIEHL-JONES AND N. C. BOLS 6%) (Bertotto et al, 1990(Bertotto et al, , 1991. Even fewer natural killer (NK) cells are found in human milk (Wirt et al, 1992), a fact consistent with the relatively low cellular cytotoxic activity of milk cells (Kohl et al, 1980).…”

Section: Milk Cell Types and Functionsmentioning

confidence: 99%

Use of Response Biomarkers in Milk for Assessing Exposure to Environmental Contaminants: The Case for Dioxin-Like Compounds

Diehl-Jones

Bols

2000

Journal of Toxicology and Environmental Health, Part B

View full text Add to dashboard Cite

Screening for environmental contaminants in milk is generally conducted by chemical analysis, yet such an approach may be time-consuming and expensive, and is not indicative of the physiological consequences of such exposure. The focus of this review is to summarize those constituents of milk that may be altered by maternal exposure to one of the most biologically active environmental pollutants, 2,3,7,8-tetrachlorodibenzo-p-dioxin, and to develop the case for their use as biomarkers of response. Several chemical and/or cellular components of milk are potentially useful as biomarkers, and may be developed as convenient, biologically relevant indicators of maternal exposure to dioxin-like compounds.

show abstract

Lymphocytes bearing the T cell receptor gamma delta in human breast milk.

Abstract: Lymphocytes bearing the T cell receptor yb (TCR-yb) were searched for in human early milk lymphocyte suspensions by two colour cytofluorimetric analysis. It was found that the proportion of TCR-yb' ceils was twofold

Cited by 37 publications

References 5 publications

Multiple independent lineages of HIV-1 persist in breast milk and plasma

Multiple independent lineages of HIV-1 persist in breast milk and plasma

Phagocytosis-Promoting Factor in Human Colostrum

Use of Response Biomarkers in Milk for Assessing Exposure to Environmental Contaminants: The Case for Dioxin-Like Compounds

Contact Info

Product

Resources

About