Phagocytosis-Promoting Factor in Human Colostrum

Straussberg, R; Sirota, Lev; Hart, John; Amir, Yazdanparast Seyed; Djaldetti, M; Bessler, Hanna

doi:10.1159/000244212

Cited by 14 publications

(8 citation statements)

References 9 publications

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“…This result indicates that phagocytosis-promoting factor(s) are present in bovine colostrum. It has reported that human colostrum has a phagocytosis-promoting factor [21] and colostral mononuclear phagocytes are able to kill enteropathogenic Escherichia coli opsonized with human colostral IgA [22]. Thus, there is a possibility that colostrum possess either opsonizing or stimulating effect on phagocytosis.…”

Section: Discussionmentioning

confidence: 99%

Promoting Effect of Colostrum on the Phagocytic Activity of Bovine Polymorphonuclear Leukocytes in vitro

2001

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Bovine colostrum contains a variety of essential nutrients, antibodies, cytokines, hormones, and growth factors that are important for nutrient supply, host defense, growth and for general neonatal adaptation. We have investigated the effect of bovine colostrum on the phagocytic activity for latex particles by normal peripheral blood polymorphonuclear leukocytes using flow cytometric analysis. The phagocytosis promoting effect was observed in colostrum. The promoting activity showed dose-dependent increase up to 25% at a concentration of colostrum. This activity was maximum in the colostrum obtained at parturition and gradually decreased with the time course of lactation as well as IgG level. Colostrum possessed the higher promoting activity than milk and normal serum. These results indicate that bovine colostrum strongly activates polymorphonuclear leukocyte phagocytosis, suggesting the concernment with development of nonspecific immune system in newborns.

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Section: Discussionmentioning

confidence: 99%

Promoting Effect of Colostrum on the Phagocytic Activity of Bovine Polymorphonuclear Leukocytes in vitro

2001

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“…Bovine IgGs and IgM enhance the phagocytic activity of bovine leucocytes by opsonising bacteria, but IgA lacks such an activity. Human colostrum affects human phagocytes -both inhibition [28] and activation [29] have been reported -and bovine whey proteins may inhibit the phagocytic activity of bovine leucocytes [ 121 but, to our knowledge, there are no reports about their effects on human leucocytes. However, passive immunisation regimens based on bovine colostrum are developed against many microbial diseases and, therefore, interactions between bovine whey proteins and human immune defence cells, e.g.…”

Section: Killing Assaysmentioning

confidence: 99%

Colostral proteins from cows immunised with Streptococcus mutans/S. sobrinus support the phagocytosis and killing of mutans streptococci by human leucocytes

Loimaranta¹,

Nuutila

Marnila

et al. 1999

Journal of Medical Microbiology

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Passive immunisation, based on bovine colostral preparations, is an area of active research. Specific bovine antibodies inhibit the virulence factors of target pathogens but the interactions between whey preparations and human immune defence cells are not well known. Bovine colostrum inhibits the phagocytic activity of bovine leucocytes and this may reflect the biological activity of immunoglobulins in it. Therefore, this study aimed to examine the effects of bovine whey protein preparations from the colostrum of Streptococcus mutuns/S. sobrinus-immunised and sham-immunised cows on binding, ingestion and killing of these bacteria by human leucocytes. Binding and ingestion of FITC-labelled bacteria were estimated by flow cytometry and leukocyte activation was measured as chemiluminescence. Killing rate was estimated by plate counting and by measuring bioluminescence from S. mutans-containing the insect luciferase gene. Colostral whey protein preparation from hyperimmunised cows activated human leucocytes by opsonising specific bacteria. Neutrophils, eosinophils and monocytes weakly phagocytosed non-opsonised bacteria and bacteria opsonised with control product. On the contrary, binding and ingestion were efficient in the presence of the preparation from immunised cows. Thus, these results show that bovine colostral whey proteins are able to support the activation of human phagocytes against pathogenic microbes and that this property is related to specific antibodies in whey preparations. These whey proteins may also be clinically useful, especially in preventing the colonisation of newly erupted teeth by mutans streptococci.

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“…Their presence in the colostral milk in numbers reciprocal with the maturity of the newborn is an indicator of their importance for the baby's immune defense (Jain et al, 1991). During the early postpartum period when the immune system of the newborn is not fully developed, human colostrum contains a phagocytosis-promoting factor(s), capable of increasing both the number and individual activity of peripheral blood phagocytic cells from normal adults (Straussberg et al, 1995). Moreover, this factor was found to be more active in preterm colostrum, providing reinforcement for the underdeveloped immune system of the preterm newborn.…”

Section: Introductionmentioning

confidence: 99%

Phagocytosis—The mighty weapon of the silent warriors

Djaldetti

Salman

Bergman

et al. 2002

Microscopy Res & Technique

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Professional phagocytes, comprising polymorphonuclear neutrophils and monocyte/macrophage cells, play an important role in the host defense. Any defect in their function exposes the organism to microbial intruders terminating in fatal diseases. The functional responses of the phagocytes to bacterial and fungal infections include chemotaxis, actin assembly, migration, adhesion, aggregation, phagocytosis, degranulation, and reactive oxygen species production. Superoxide generation by phagocytic NADPH oxidase is an imperative step toward bacterial killing. Phagocytes participate in inflammatory reactions and exert tumoricidal activity. They are supported by serum factors such as immunoglobulins, cytokines, complement, the acute phase reactant C-reactive protein, production of antibacterial proteins, and others. In addition to their principal task to eliminate bacteria, they are engaged in removing damaged, senescent, and apoptotic cells. Engulfed cell debris, large particles such as latex beads, fat, and oil droplets, are examples of phagocytic activity illustrated in the present review with transmission and scanning electron microscope micrographs. Numerous factors, such as diseases and stressful conditions, affect the engulfing activity of the professional phagocytes. Our experience regarding the impaired phagocytic capacity of cells in patients with diabetes and chronic renal failure is discussed. The results obtained in our laboratory from experiments detecting the effect of strenuous physical exercise, hypothermia, fasting, and abdominal photon irradiation on the phagocytic capacity of human polymorphonuclear neutrophils and rat peritoneal macrophages are hereby summarized and the reports on those subjects in the recent literature are reviewed. A variety of assays are applied for quantifying phagocytosis. Flow cytometry based on incubation of phagocytic cells with fluorescent conjugated particles and measuring the amount of fluorescence as an indicator of the engulfing capacity of the cells is a useful method. A direct visualization of the ingested particles using light or electron microscopy is a valuable tool for estimation of phagocytic function. In our hands, the use of semithin sections of embedded phagocytes following their incubation with latex particles provided satisfactory results for measuring the total number of phagocytic cells, as well as the internalizing capacity of each individual cell. Microbiological assays, the nitroblue tetrazolium test, quantitation of antibody- and antigen-mediated phagocytosis, as well as methods reviewed in detail in other reports are additional applications for determination of this intricate process.

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Phagocytosis-Promoting Factor in Human Colostrum

Cited by 14 publications

References 9 publications

Promoting Effect of Colostrum on the Phagocytic Activity of Bovine Polymorphonuclear Leukocytes in vitro

Promoting Effect of Colostrum on the Phagocytic Activity of Bovine Polymorphonuclear Leukocytes in vitro

Colostral proteins from cows immunised with Streptococcus mutans/S. sobrinus support the phagocytosis and killing of mutans streptococci by human leucocytes

Phagocytosis—The mighty weapon of the silent warriors

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