Lymphocyte transformation in malignant lymphomas

Papac, Rose J.

doi:10.1002/1097-0142(197008)26:2<279::aid-cncr2820260206>3.0.co;2-t

Cited by 42 publications

(9 citation statements)

References 27 publications

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“…The absolute number of these nonresponsive cells was increased in 2 FLL cases. Similar results have been obtained in patients with well-differentiated lymphocytic lymphomas (Quaglino and Cowling, 1964;Lawler et al, 1968;Rubin et al, 1969;Papac, 1970;Liknaitzky et al, 1970). These cells appear to be analogous to the non-transforming cells of CLL.…”

Section: Discussionsupporting

confidence: 79%

“…The data of Rubin et al (1969) suggest that they do so in 5 or 6 days, thus also supporting the concept that the abnormal CLL cells correspond in the normal with the PHA-responsive population of lymphocytes. However, this view is not supported by the findings of Wilson and Nossal (1971) and those of Papamichail et al (1971), who have demonstrated immunoglobulins on the surface of CLL lymphocytes which resembled the findings in the B-lymphocytes. Thus, the question of the B or T nature of these cells is still open (Catovsky and Holt, 1971).…”

Section: Discussionmentioning

confidence: 71%

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Lymphocyte Transformation in Immunoproliferative Disorders

Catovsky¹,

Holt²,

Galton³

1972

Br J Cancer

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Summary.-Lymphocyte transformation with phytohaemagglutinin (PHA) was studied in 30 patients with immunoproliferative disorders (lymphoproliferative and plasma cell disorders).Lymphocyte transformation at 3 days was reduced in the lymphoproliferative disorders (chronic lymphocytic leukaemia (CLL), well-differentiated (lymphocytic) follicular lymphoma (FLL) and Waldenstrom's macroglobulinaemia (WM)), and normal in the plasma cell disorders (myelomatosis, primary systemic amyloidosis, a-chain disease and benign monoclonal gammopathy) and in idiopathic cold haemagglutinin disease. A case of plasma-cell leukaemia with increased numbers of abnormal cells in the circulation also showed reduced transformation. It is suggested that the presence in the circulation of abnormal lymphocytes (or plasma cells) accounts for the results in CLL and FLL, WM and plasma-cell leukaemia. In WM a correlation was found between the activity of the disease (expressed by the levels of IgM paraprotein) and the degree of blast transformation.The long-term (28 days) in vitro survival of lymphocytes using subconcentrations of PHA was also studied in 7 patients. The cell populations (PHA-non-responsive) of CLL and FLL, but not of WM, had a good in vitro survival, resembling in this respect the normal PHA-responsive population of lymphocytes, but they remained PHA-non-responsive after 4 weeks' culture. It is speculated that in CLL the long survival in vitro of the PHA-non-responsive (leukaemic) population corresponds to their long life-span in vivo.

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Section: Discussionsupporting

confidence: 79%

Section: Discussionmentioning

confidence: 71%

Lymphocyte Transformation in Immunoproliferative Disorders

Catovsky¹,

Holt²,

Galton³

1972

Br J Cancer

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“…The immunologic defect of early active HD is characterized by depression of delayed hypersensitivity and decreased homograft reactions (2,3). Antibody formation is largely intact and peripheral lymphocyte counts are normal or slightly decreased, while a loss of phytohemagglutinin (PHA) responsiveness of lymphocytes in HD has been reported even before peripheral lymphocyte depletion has occurred (4). Thus a defect of T-lymphocyte reactivity is well established, but its role in the pathogenesis of the disease itself is uncertain.…”

Section: Introductionmentioning

confidence: 99%

Studies of Cell Subpopulations Mediating Mitogen Hyporesponsiveness in Patients with Hodgkin's Disease

Sibbitt¹,

Bankhurst²,

Williams³

1978

J. Clin. Invest.

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A B S T R A C T Hodgkin's disease (HD) is associated with a deficit in T-cell immunity characterized by skin test anergy and decreased lymphocyte responses to phytohemagglutinin (PHA). To investigate this mitogen hyporesponsiveness in HD, we separated peripheral blood mononuclear cells on Ficoll-Hypaque gradients and determined their response to various suboptimal concentrations of PHA. As was expected, patients with HD demonstrated marked mitogen hyporesponsiveness relative to normal controls; however, if the cell suspensions were first passed through glass wool columns to remove adherent cells, the PHA responsiveness of the hyporesponsive HD cells was markedly increased. In contrast, the responsiveness of normal controls was decreased so that the responses of nonadherent normal and HD cells were statistically indistinguishable. Evidently, a glass wool-adherent suppressor cell had been removed from patients with HD, while a glass wool-adherent cell which enhanced mitogenic responses had been removed from normal controls during column passage. Previous to column depletion, patients with HD had decreased proportions of E-rosettes and increased proportions of cells with surface a-fetoprotein; however, the proportion of these cells was not changed after column passage. Significant changes with column depletion of glass wool-adherent cells in HD were recorded in the proportions of monocytes (13.2 vs 5.8%) and lymphocytes with C-3 receptors (12.6 vs. 7.8%). The only significant change in normal controls was a decrease in the proportion of monocytes (10 vs. 1.7%). To determine ifglass-adherent cells would have a suppressor effect, HD-adherent cells were added in progressively increasing numbers to mononuclear cell suspensions depleted of glass wool-adherent cells. PHA responsiveness returned Mr.

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“…WHILST depression of the PHA response in chronic lymphatic leukaemia (Smith, Cowling and Barker, 1972), Hodgkin's disease (Trubowitz, Masek and Del Rosario, 1966) and other malignant lymphomata (Papac, 1970) is now well established, the change in non-lymphoid malignancies remains in doubt. Most reports indicate that a similar depression occurs in the non-lymphoid malignancies (Silk, 1967;Ducos et al, 1970;Whittaker, Rees and Clark, 1970;Hann and Takita, 1972) but there are conflicting data indicating that the response may be normal (Robinson and Hurvitz, 1966;Sutherland, Inch and McCredie, 1971;Nelson, 1969).…”

mentioning

confidence: 99%