Lymphocyte transformation and chromosome studies in Hodgkin's disease.

Lawler, Sylvia D.; Pentycross, C. R.; Reeves, Brian

doi:10.1136/bmj.3.5567.704

Cited by 26 publications

(8 citation statements)

References 14 publications

(5 reference statements)

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“…In reticulum cell sarcoma, Stage IV, the impairment of transformation is remarkably similar to that observed in advanced cases of Hodgkin's disease, observed in this series, as well as that reported by others.3, 15, 17 T r u b o w i t P studied 8 patients with lymphosarcoma and reported that blast response to PHA did not differ from the control, although the data suggests reduction from control values in 3 cases. T h e differences observed between the control subjects, and patients with Hodgkin's disease and reticulum cell sarcoma were significant, in contrast to cases of lymphosarcoma.…”

Section: Discussionsupporting

confidence: 89%

Lymphocyte transformation in malignant lymphomas

Papac

1970

Cancer

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Peripheral blood leukocyte cultures with phytohemagglutinin (PHA) were studied in 19 patients with malignant lymphomas, classified as Stage IV. Thirteen patients had lymphosarcoma and 6 had reticulum cell sarcoma. Control subjects included both healthy and chronically ill subjects. Seventeen patients with Hodgkin's disease were also studied. A wide range of responsiveness to PHA was observed in cases of lymphosarcoma with a median value for the group lower than that of control subjects. Correlation of lymphocyte transformation with PHA was noted with histologic type of lymphosarcoma, with duration of disease and with age of the patient. The most marked impairment of transformation generally occurred in patients with small cell lymphosarcoma, in patients with brief duration of disease and older age groups. There was little correlation between peripheral blood lymphocyte level and percentage transformation with PHA. In patients with reticulum cell sarcoma, PHA stimulation was quite markedly impaired. In Hodgkin's disease, impaired transformation with PHA was related to stage of disease and peripheral lymphocyte levels. Patients with Stage IV disease showed decreased transformation as did patients with lymphopenia.

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Section: Discussionsupporting

confidence: 89%

Lymphocyte transformation in malignant lymphomas

Papac

1970

Cancer

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“…The depression of PHA responsiveness persists for several months and it is also demonstrable in patients who had been continuously free from disease for more than 1 year after the initial course of treat ment. These data are at variance with those of Lawler et al [1967] and of H an and Sokal [1970], who showed normal lymphocyte responses within 3 months of completing a course of radiation, and suggest that the abnormal responses in remission patients, also noted by other workers [Levy and Kaplan, 1974], may be iatrogenic rather than disease related. However, in our study, remission patients showed generally better respon ses than patients who, at the time of the test, were relapsed.…”

Section: Discussioncontrasting

confidence: 55%

<i>In vitro</i> Lymphocyte Response to Phytomitogens in Untreated and Treated Patients with Hodgkin’s Disease

Romagnani

Amadori

Biti

et al. 1976

Int Arch Allergy Immunol

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In vitro lymphocyte response to phytohemagglutinin (PHA), concavalin A (Con A), and pokeweed mitogen (PW) was evaluated in untreated and treated patients with Hodgkin’s disease (HD). The responding capacity to PHA was depressed, though not constantly, in the untreated patients compared with the response of lymphocytes from normal individuals. The depression was more evident, at group level, when the cells were stimulated with suboptimal concentrations of PHA. Radiotherapy constantly induced a strong decrease or a complete loss of the responding capacity of the cells which persisted at low levels for many months. Some years after the initial course of treatment, the response was clearly depressed, but it was better in patients in remission than during relapse. Splenectomy did not affect the responding capacity of the cells. The depressive effect induced by chemotherapy was apparently less marked and persistent than that of radiation. Con A-and PW-induced lymphocyte transformation usually paralleled the PHA-induced response. The depressed response to PHA was not due to an inhibitory activity of HD serum. Washed HD lymphocytes in fetal calf serum were not stimulated better than in autologous plasma, nor were HD sera able to depress the response of normal lymphocytes to PHA, Con A, PW, and PPD. Supernatants from HD lymphocytes cultured for 24 h without any stimulant, and extracts of these cells were also unable to affect the response of normal lymphocytes to PHA.

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“…The data from which the figure was compiled include cases additional to those recorded here (Pentycross, 1968c). The cases with Hodgkin's disease have been published previously (Lawler et al, 1967).…”

Section: Transformation Studiesmentioning

confidence: 99%

Chromosomes and Transformation of Lymphocytes in Lymphoproliferative Disorders

Lawler¹,

Pentycross²,

Reeves³

1968

BMJ

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Cardiac Surgery in Children-Harden et al.BRITISH 213 effect on cerebral electrogenesis. The relation between plasma electrolyte changes and increase in slow activity in the E.E.G. showed some individual variations, but we were not able to measure total body or urinary electrolyte values, osmolality of plasma or urine, or the rate at which these metabolic disturbances evolved. Differences in intravenous fluids given (5% dextrose, 0.09 or 0.18% NaCl/dextrose solution) did not affect substantially the general cycle of events. Neither the patient's age nor the type of operation appeared to influence the degree or the timing of the postoperative metabolic and E.E.G. changes.In conclusion, it has been our experience that a generalized increase in slow activity in the E.E.G. occurs between the first and the fourth day after heart surgery. The E.E.G. changes are fully reversible, in contrast to the effects of severe cerebral anoxia. The Brit. med. 7., 1968, 4, 213-220 Summary: In chronic lymphocytic leukaemia the majority of circulating lymphocytes which responded to phytohaemagglutinin in vitro were found to have normal karyotypes. A minor population of cells in patients treated with chemotherapy had an increased number of chromosomal rearrangements as compared with cells from normal controls and untreated patients with chronic lymphocytic leukaemia. Probably bonemarrow and lymph-node cells also had a normal karyotype.In the other lymphoproliferative disorders the peripheral blood lymphocytes had either normal karyotypes or chromosomal abnormalities attributable to treatment, even in those cases where the tumour cells of involved lymph nodes were known to have abnormal karyotypes.It was possible that circulating tumour cells were present in one case.

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Lymphocyte transformation and chromosome studies in Hodgkin's disease.

Cited by 26 publications

References 14 publications

Lymphocyte transformation in malignant lymphomas

Lymphocyte transformation in malignant lymphomas

<i>In vitro</i> Lymphocyte Response to Phytomitogens in Untreated and Treated Patients with Hodgkin’s Disease

Chromosomes and Transformation of Lymphocytes in Lymphoproliferative Disorders

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