Lymphocyte‐Mediated Cytotoxicity Against Tumor Cells Specificity and Characterization of Concanavalin A‐Activated Cytotoxic Effector Lymphocytes

Waterfield, J D; Waterfield, Elizabeth; Anaclerio, Alessandro; Möller, Göran

doi:10.1111/j.1600-065x.1976.tb00205.x

Cited by 14 publications

(13 citation statements)

References 43 publications

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“…Similarly t o the effect of LPS on B cells, therefore [33, 341, Con A induces a polyclonal effector response in T cells in the absence of added antigen and the induced cells retain their predetermined specificity. Clark 1351 and Waterfield et al [36] have recently reported similar findings of Con A-induced specific cytotoxicity.…”

Section: Discussionsupporting

confidence: 55%

H‐2 antigen‐specific cytotoxic T cells induced by concanavalin A: estimation of their relative frequency

1976

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Specific and nonspecific lysis of DBA/2 (H-2d) mastocytoma cells, P815, by concanavalin A (Con A)-induced cytotoxic T cells was studied. In the assay for nonspecific lysis, phytohemagglutinin (PHA) was present to glue the target and killer cells together. We have presented evidence previously to show that PHA reveals only, and all, cytotoxic T cells. In the assay for specific lysis the only glue present was specific receptors on a fraction of the killer cells and surface antigens of P815. We show that when PHA was present, Con A-induced cells which were syngeneic, semi-syngeneic, or allogeneic, lysed P815 very efficiently in a 4-h 51Cr release assay. However, only Con A-induced T cells which were allogeneic and did not carry H-2d lysed P815 when the assay was carried out in the absence of PHA. In an experiment with two target cells, Con A-induced B10 (H-2b) T cells lysed B10.D2 (H-2d) targets specifically but did not lyse B10 targets, while Con A-induced B10.D2 T cells lysed B10 targets specifically but not B10.D2 targets. Furthermore, Con A-induced B6 (H-2b) T cells from normal mice lysed P815 specifically but Con A-induced B6 T cells from irradiated F1 (B6 x BALB/c) (H-2b/d) mice reconstituted with B6 bone marrow did not lyse P815 specifically. A fraction of Con A-induced T cells therefore appear to bear specific surface receptors for nonself H-2 coded structures. We describe conditions of assay and a new method of plotting the results such that nonspecific (PHA-revealed) and specific (PHA-independent) cytotoxicity can be quantitatively compared. We conclude that 1-4% of the total Con A-induced cytotoxic effector T cells are directed against any particular foreign H-2 haplotype. This is the first estimate of the relative frequency of antigen-reactive cytotoxic T cells.

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Section: Discussionsupporting

confidence: 55%

H‐2 antigen‐specific cytotoxic T cells induced by concanavalin A: estimation of their relative frequency

1976

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“…The response is polyclonal in that mitogens stimulate a large fraction of all T cells independently of V gene expression in individual T cells [2][3][4].…”

Section: Introductionmentioning

confidence: 99%

Studies on T lymphocyte activation I. Requirements for the mitogen‐dependent production of T cell growth factors

Andersson¹,

Grönvik²,

Larsson

et al. 1979

Eur J Immunol

130

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Conditioned media (CM) obtained from concanavalin A (Con A)-stimulated spleen cell cultures maintain mitogen-derived T cell blasts in exponential growth for indefinite periods of time. Such purified T cell blasts do not respond to Con A, and the growth-promoting activity in CM is independent of the mitogenic lectin used for its production. However, the appearance of activity in CM is Con A dose-dependent. Furthermore, the production of these T cell growth factors is independent of B cells, while it requires both T cells and nontheta-bearing, probably Ia-positive cells present in normal and nude spleens. On the other hand, the T cell blasts which are reactive to the growth factors, neither produce CM by themselves nor can they cooperate with nude spleen cells for its production, in the presence of a mitogenic lectin. Since the concentration of growth factors in CM determines the extent of T cell growth, we propose that the growth of some T cells is necessarily dependent upon the activity in CM described here and, consequently, that the basis for T cell activation is the induction of such growth factors.

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“…It has been considered that the role of Con A in Con A-induced cellular cytotoxicity is not only to activate effector lymphocytes but also to result in an actual binding of effector lymphocytes to target cells via a Con A 'bridge' (9,17,21). In this cellular cytotoxicity it was, however, difficult to detect soluble cytotoxic factors released from activated effectors by any kind of target cells.…”

Section: Discussionmentioning

confidence: 98%

“…Mitogen-dependent cytotoxicity of T lymphocytes in vitro has been in vestigated as useful models for analysis of the cytotoxic mechanism of activated T lymphocytes (7,9,12,13,17,18,21). A soluble cytotoxic factor termed 'lymphotoxin' was identified in the culture supernatant of T lymphocytes ac tivated by phytomitogens or soluble antigens (1,4,10,19).…”

Section: Introductionmentioning

confidence: 99%

Role of a Soluble Cytotoxic Factor in Concanavalin A-Induced Cellular Cytotoxicity to Rabbit Erythrocytes

Nose¹,

Kyogoku

1979

Pathobiology

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A new sensitive and simplified method for detecting concanavalin A-induced cellular cytotoxicity was developed by using ⁵¹Cr-labeled rabbit erythrocytes as target cells, and a soluble cytotoxic factor (SCF) to rabbit erythrocytes was demonstrated with this system. From morphological studies, this factor appeared to play a major role in cellular cytotoxicity. The cytotoxic activity of SCF was removed by absorption with rabbit erythrocytes. Furthermore, the target cells pretreated with SCF and washed were subsequently destroyed. These results may indicate that SCF exerts its effect through binding to cellular receptors. Some monosaccharides showed inhibitory effects on the cytolysis of rabbit erythrocytes by SCF and on the adsorption of SCF activity to them. The significance of these findings in elucidation of the action of SCF was discussed.

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Lymphocyte‐Mediated Cytotoxicity Against Tumor Cells Specificity and Characterization of Concanavalin A‐Activated Cytotoxic Effector Lymphocytes

Cited by 14 publications

References 43 publications

H‐2 antigen‐specific cytotoxic T cells induced by concanavalin A: estimation of their relative frequency

H‐2 antigen‐specific cytotoxic T cells induced by concanavalin A: estimation of their relative frequency

Studies on T lymphocyte activation I. Requirements for the mitogen‐dependent production of T cell growth factors

Role of a Soluble Cytotoxic Factor in Concanavalin A-Induced Cellular Cytotoxicity to Rabbit Erythrocytes

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