Role of a Soluble Cytotoxic Factor in Concanavalin A-Induced Cellular Cytotoxicity to Rabbit Erythrocytes

Abstract

A new sensitive and simplified method for detecting concanavalin A-induced cellular cytotoxicity was developed by using ⁵¹Cr-labeled rabbit erythrocytes as target cells, and a soluble cytotoxic factor (SCF) to rabbit erythrocytes was demonstrated with this system. From morphological studies, this factor appeared to play a major role in cellular cytotoxicity. The cytotoxic activity of SCF was removed by absorption with rabbit erythrocytes. Furthermore, the target cells pretreated with SCF and washed were subsequently destroyed. These results may indicate that SCF exerts its effect through binding to cellular receptors. Some monosaccharides showed inhibitory effects on the cytolysis of rabbit erythrocytes by SCF and on the adsorption of SCF activity to them. The significance of these findings in elucidation of the action of SCF was discussed.

K Cell Activity of Murine-Cultured Lymphocytic Leukemic Cell Line Independent of Other Fc Receptor Functions

“…As described in our previous report [8], fresh rabbit erythrocytes (IX 10s cells), washed three times in Hanks balanced salt solution (HBSS), were incubated with 200 ,u d of sodium ^chro mate (spec. act.…”

“…Cytotoxicity was determined by measuring the release of 51Cr from target cells as described else where [8]. SC-1 cell (1X106) were incubated with target cells (1X106) in the presence of various kinds of antibodies on a tissue culture plate (Mi cro Test II, Falcon 3040).…”

Steps toward the New Horizons of Immunopathology Continuing from the Reach of My Research Career.

“…Many young researchers joined my new laboratory at Kobe University and helped in doing detailed in vitro experiments. Among them, Dr. Masato Nose was inspired with the importance of cell surface sugar chains during the course of our analysis on immunocytolysis of cultured heart muscle cells (Kyogoku et al 1975; Koide et al 1977;Nose and Kyogoku 1979;Nose et al 1981). He still seems to be pursuing the same idea after having collaborated with Prof. Hans Wigzell at the Karolinska Institute to study the role of carbohydrate chains of immunoglobulins (Nose and Wigzell 1983;Ohuchi et al 1984Ohuchi et al , 1986Nose et al 1987aNose et al , 1988Nose et al , 1990a As I mentioned above, I moved to Kobe in 1971 being assigned as Chairman and Professor of the Department of Pathology, and had a chance to become a section chief of a nationwide project team under the auspice of Prof. Yuichi Shiokawa.…”