Lymphocyte blastogenic responses of calves experimentally infected with Ostertagia ostertagi

Cross, D.A.; Klesius, Phillip H.; Haynes, T.B.

doi:10.1016/0304-4017(86)90006-3

Cited by 15 publications

(5 citation statements)

References 6 publications

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“…There was also demonstrated that T lymphocytes from T. colbriforms infected sheep (46) and Ostertagia ostertagi infected cattle (47) showed in vitro decrease of the proliferative response to mitogen. Severe helminthiasis was also observed when sheep was submitted to immunosuppression by cyclophosphamide treatment (12).…”

Section: Discussionmentioning

confidence: 98%

Adjuvant effect of LPS and killedPropionibacterium acneson the development of experimental gastrointestinal nematode infestation in sheep

Abel

Chen

Ricca

et al. 2009

Parasite Immunology

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Gastrointestinal helminthic infection is an important worldwide sheep disease. The emergence of anthelminthic resistance has led to drives to seek new means of therapeutic control of helminthiasis in sheep. Several data demonstrated the adjuvant effect of Propionibacterium acnes on resistance to infection. Herein, we evaluate the adjuvant effect of the commercial suspension containing LPS and P. acnes on experimental helminthiasis. Sheep received three doses of LPS and P. acnes commercial suspension or saline 0.9% (control group). Both groups received orally Haemonchus contortus infective larvae on day 0. Parasitological, haematological, lymphoproliferation analysis, IL-5 and IgE determination were made once a week until 35th day after infection. Our results revealed increase on packed cell volumes on day 14, in LPS + P. acnes treated group. On 21st and 35th days after infection in the same group occurred increase on circulating eosinophils and lymphocytes, and also in the lymphoproliferative response to mitogen. On 35th day, the faecal eggs peak in LPS + P. acnes treated group was significantly lower than control. A negative correlation between faecal eggs counts and circulating eosinophils in the immunostimulant treated group was also observed. Our findings suggest that LPS + P. acnes suspension can be used as a strategy to control helminthiasis in sheep.

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Section: Discussionmentioning

confidence: 98%

Adjuvant effect of LPS and killedPropionibacterium acneson the development of experimental gastrointestinal nematode infestation in sheep

Abel

Chen

Ricca

et al. 2009

Parasite Immunology

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“…While there is clear evidence from human, rodent and pig studies that nematode infections can impact on vaccine responsiveness and susceptibility to other infectious diseases [ 77 , 92 ], for ruminant species the data is less clear. Early studies of O. ostertagi infections in cattle identified a transient non-specific suppression of cellular immune responses during the early stages of infection [ 15 , 85 , 97 , 98 ], suggesting that infections with this nematode result in general impairment of adaptive host immunity. Consistent with this observation, antibody responses to Brucella abortus and Infectious Bovine Rhinotracheitis virus (IBRV) vaccines were slower to develop in cattle coinfected with O. ostertagi [ 97 ], although these results were not replicated in a subsequent trial using mixed O. ostertagi and Cooperia onchophora infections [ 98 ].…”

Section: Impact Of Helminth Infections On Host Immune Competencementioning

confidence: 99%

Immune modulation by helminth parasites of ruminants: implications for vaccine development and host immune competence

McNeilly

Nisbet

2014

Parasite

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Parasitic helminths reside in immunologically-exposed extracellular locations within their hosts, yet they are capable of surviving for extended periods. To enable this survival, these parasites have developed complex and multifaceted mechanisms to subvert or suppress host immunity. This review summarises current knowledge of immune modulation by helminth parasites of ruminants and the parasite-derived molecules involved in driving this modulation. Such immunomodulatory molecules have considerable promise as vaccine targets, as neutralisation of their function is predicted to enhance anti-parasite immunity and, as such, current knowledge in this area is presented herein. Furthermore, we summarise current evidence that, as well as affecting parasite-specific immunity, immune modulation by these parasites may also affect the ability of ruminant hosts to control concurrent diseases or mount effective responses to vaccination.

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“…Studies performed in vivo, including identification of lymphocyte subpopulations in the draining lymph nodes, have shown a decrease in the percentage of CD4+ lymphocytes in non-immune animals after infection (Cross et al, 1986). This coincides with a local increase in IgM-bearing cells and a decrease in CD3+ cells (Almeria et al, 1997b;Gasbarre, 1994).…”

Section: Introductionmentioning

confidence: 95%

“…Proposed mechanisms have included the generation of suppressor T cells, a generalized non-specific hyporesponsiveness, and polyclonal lymphocyte activation (Klesius, 1993;Yang et al, 1993;Gasbarre, 1997). In addition, it has been extremely difficult to induce antigen-specific proliferation when adding specific antigens to cultures taken from infected cattle (Cross et al, 1986;Snider et al, 1986).…”

Section: Introductionmentioning

confidence: 99%

Inhibition of bovine T lymphocyte responses by extracts of the stomach worm Ostertagia ostertagi

Gómez-Muñoz

Canals-Caballero²,

Almerı́a

et al. 2004

Veterinary Parasitology

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Lowered immune responses during bovine ostertagiosis have been reported in both in vivo and in vitro assay systems. In the present study we have employed three different life cycle stages of the nematode Ostertagia ostertagi to determine if products of this economically important parasite inhibit in vitro proliferation of Con A-stimulated cells from uninfected animals. We have demonstrated an inhibitory effect upon the growth of Con A-stimulated lymphocytes after addition of fourth stage larval (L4) soluble extract (L4SE) to the cultures. In contrast, extracts from the third stage larvae (L3) had little or no inhibitory activity. The suppressive products were also shown to be secreted by the late L4. The suppressive activity is reversible if the L4 products are removed from culture. There is no immediate effect on proliferating cells and the L4SE must be in culture for 24-48 h before suppression is observable. The L4SE caused slight but not statistically significant decreases in the percentage of T cells and increases in B cell percentages in cultures when compared with cultures stimulated with Con A alone. No changes were seen in percentage of cells positive for markers for CD4, CD8, gammadelta T cells, or monocytes/macrophages as a consequence of the addition of L4SE. In contrast, there was a strong and significant reduction in the expression of the IL-2 receptors in cells cultured in the presence of the worm extract. There was no evidence of either necrosis or apoptosis resulting from the presence of L4 products in culture. The expression of messenger RNA for interleukin-2, -4, -13, tumor necrosis factor-alpha (TNF-alpha), and gamma-interferon (gamma-IFN) was decreased when L4SE was included in cultures of Con A-stimulated cells compared to cultures stimulated with Con A only. In contrast, messenger RNA expression of transforming growth factor-beta (TGF-beta) and interleukin-10 (IL-10) was increased in cells growing in the presence of L4 products. The potential role of these cytokines during ostertagiosis is discussed.

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Lymphocyte blastogenic responses of calves experimentally infected with Ostertagia ostertagi

Cited by 15 publications

References 6 publications

Adjuvant effect of LPS and killedPropionibacterium acneson the development of experimental gastrointestinal nematode infestation in sheep

Adjuvant effect of LPS and killedPropionibacterium acneson the development of experimental gastrointestinal nematode infestation in sheep

Immune modulation by helminth parasites of ruminants: implications for vaccine development and host immune competence

Inhibition of bovine T lymphocyte responses by extracts of the stomach worm Ostertagia ostertagi

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