Adjuvant effect of LPS and killedPropionibacterium acneson the development of experimental gastrointestinal nematode infestation in sheep

Abstract

Gastrointestinal helminthic infection is an important worldwide sheep disease. The emergence of anthelminthic resistance has led to drives to seek new means of therapeutic control of helminthiasis in sheep. Several data demonstrated the adjuvant effect of Propionibacterium acnes on resistance to infection. Herein, we evaluate the adjuvant effect of the commercial suspension containing LPS and P. acnes on experimental helminthiasis. Sheep received three doses of LPS and P. acnes commercial suspension or saline 0.9% (control group). Both groups received orally Haemonchus contortus infective larvae on day 0. Parasitological, haematological, lymphoproliferation analysis, IL-5 and IgE determination were made once a week until 35th day after infection. Our results revealed increase on packed cell volumes on day 14, in LPS + P. acnes treated group. On 21st and 35th days after infection in the same group occurred increase on circulating eosinophils and lymphocytes, and also in the lymphoproliferative response to mitogen. On 35th day, the faecal eggs peak in LPS + P. acnes treated group was significantly lower than control. A negative correlation between faecal eggs counts and circulating eosinophils in the immunostimulant treated group was also observed. Our findings suggest that LPS + P. acnes suspension can be used as a strategy to control helminthiasis in sheep.

Immunostimulatory activity of exopolysaccharides from probiotic Lactobacillus casei WXD030 strain as a novel adjuvant in vitro and in vivo

“…Various approaches have been developed recently in order to improve vaccine potency, particularly with the respect to choose of a better adjuvant. To date, despite various adjuvants, such as lipopolysaccharide (LPS) and Freund's complete adjuvant (FCA), have been identified and extensively studied, they are not widely applied due to their toxicity (Abel et al, 2009;Pohanka, Vobornikova, & Fusek, 2016). Hence, only partial adjuvants, such as alum, are licensed for clinical use (Mbow, Gregorio, Valiante, & Rappuoli, 2010).…”

Immunization with recombinant rHc23 partially protects lambs against trickle infections by Haemonchus contortus

“…Present experiment achieved lower protection levels than those obtained, with the same recombinant, using aluminum adjuvant [7, 8]. Lack of statistical significance of differences between lambs vaccinated and those treated only with the BI (Group 1 versus group 2) does not allow to exclude the possibility of BI eliciting some unspecific protection as shown in adult animals [15] despite the lower values found in rHc23-vaccinated animals. Whether or not the lower efficacy obtained is due to the infection schedule, the immunization system (adjuvant versus immunostimulant, timing) or to the size of experimental groups should be studied.…”

Humoral immunity is the primary means of heterologous Propionibacterium acnes vaccination against Actinobacillus pleuropneumoniae infection in pig

“…At the end of last century, the commercialization agents based on P. acnes began to be used for animal immune regulation (Flaminio, Rush et al 1998). In general, P. acnes played a role in non-specific immune protection by the study of immune stimulation, such as parasites (Iseki, Takabatake et al 2008;Abel, Chen et al 2009) or viruses (Megid and Kaneno 2000). Our study for the first time showed that P. acnes for specific heterologous protective immunity could develop into a possible A. pleuropneumonia vaccine or adjuvant.…”

“…P.acnes is one kind of microorganism usually seen in nature, which is related to the inflammation in acne in human skins (Thiboutot 2008). P.acnes could enhance the resistance of animals to a variety of pathogens such as parasites (Clark, Cox et al 1977;Abel, Chen et al 2009), bacteria (Miyata, Nomoto et al 1980;Dinsmore, Cattell et al 1995) and viruses (Kirchner, Hirt et al 1977;Megid, Cremonini et al 2002), by improving the non-specific immunity of animals. In our previous work, we found that several potential vaccine candidate genes of A.pleuropneumoniae showed remarkable simiariities to those of the genes serotypes of P.acnes (Lei, Sun et al 2008).…”