Lymphatic Microvessel Density and the Expression of Lymphangiogenic Factors in Oral Squamous Cell Carcinoma

Ali, Mohammad A.

doi:10.1159/000151572

Cited by 18 publications

(14 citation statements)

References 59 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…in 2003,[16] Zheng-Jun et al . in 2007,[17] Ali in 2008[18] who concluded that VEGF expression in OSCC triggers lymphatic angiogenesis, which may result in higher risk for lymph node metastasis. The angiogenic effects of VEGF may also favor the onset of late lymphatic and hematogenous metastasis.…”

Section: Discussionmentioning

confidence: 99%

VEGF: A critical driver for angiogenesis and subsequent tumor growth: An IHC study

Kapoor¹,

Deshmukh

2012

J Oral Maxillofac Pathol

View full text Add to dashboard Cite

Background:Tumors require blood supply for their growth and dissemination. It is a well accepted paradigm that tumors recruit new blood vessels from the existing circulation (angiogenesis) and this participates in tumor invasion and metastasis. Studies in the literature provide evidence for expression of Vascular Endothelial Growth Factor (VEGF) by the tumor for neo-angiogenesis, which is not only required for the tumor growth but also its metastasis. Based on the literary evidences we carried out an Immuno-Histochemical (IHC) study for VEGF in Oral Squamous Cell Carcinoma (OSCC) tissues to provide a strong link between the factor and oral cancer.Aim:To analyze the expression of VEGF in OSCC tissues of different histological grades, clinical sizes and lymph node status and to use this as an indicator for disease progression by helping in delineating a risk population, that may benefit from an attractive adjuvant therapeutic strategy for OSCC.Settings and Design:Studies published from 1990 till 2010 have only seen the association of VEGF with tumor angiogenesis and its possible role in metastasis. This is the first study that takes into account the clinical status of the lymph nodes and VEGF expressivity in a sample size of 30 cases.Materials and Methods:30 oral squamous cell carcinoma tissue slides were stained using Hematoxylin and Eosin stain (to confirm the diagnosis) and immunohistochemically using VEGF antibody. IHC stained slides were thereafter evaluated for the positivity and intensity.Statistical Analysis:The result was subjected to statistical analysis using Chi-square testResults and Conclusion:VEGF positivity was seen in approximately. 90% of cases which was independent of histological grade of OSCC. However the intensity increased with the clinical size of cancer and from palpable lymph node to a tender and hard lymph node.

show abstract

Section: Discussionmentioning

confidence: 99%

VEGF: A critical driver for angiogenesis and subsequent tumor growth: An IHC study

Kapoor¹,

Deshmukh

2012

J Oral Maxillofac Pathol

View full text Add to dashboard Cite

show abstract

“…In tumor model overexpression of HGF was found to induce lymphatic vessel growth in the peritumoral region [14] and in human oral SCC a significant correlation was detected between LVD and HGF expression [31]. In contrast, in high grade salivary carcinomas absence of correlation between HGF expression in tumor cells and regional lymph node and distant metastasis has been reported [32].…”

Section: Discussionmentioning

confidence: 99%

Lymphatic vessel density and expressions of lymphangiogenic growth factors in salivary carcinomas

Mf¹,

Af²,

Ll³

et al. 2011

neo

View full text Add to dashboard Cite

Nodal metastasis is an important prognostic indicator in head and neck cancers, including salivary carcinomas. In these, the risk for lymph node metastasis is variable and strongly associated with the tumor histologic type. The aim of the current study was to evaluate the lymphatic vessel density (LVD) and expressions of lymphangiogenic growth factors by tumor cells in different histologic types of salivary carcinomas subdivided according to the risk for nodal metastasis. In 15 high-risk (undifferentiated, high-grade mucoepidermoid and salivary duct carcinomas) and 60 low/moderate-risk tumors (adenoid cystic, low/intermediate-grade mucoepidermoid, acinic cell, myoepithelial, epithelial-myoepithelial and polymorphic lowgrade carcinomas) the expressions of vascular endothelial growth factor-C (VEGF-C), hepatocyte growth factor (HGF) and D2-40 (for assessing LVD) were examined. No significant differences were encountered between high-and low/moderate/-risk carcinomas regarding LVD and VEGF-C or HGF expressions. Furthermore, the expression of these proteins did not correlate with LVD. Lymphatic vascular invasion was found mainly in high-risk carcinomas. Intratumoral LVD was significantly lower than peritumoral, regardless of the risk for metastasis and primary site of the lesion. The histologic types of salivary carcinomas which are associated with high-risk for nodal metastasis do not present increased LVD or VEGF-C and HGF expressions. The greater tendency for metastasis in these carcinomas seems to be related to their capacity to invade lymph vessels. Further studies on tumor cell interactions with lymphatic endothelial cells are needed to improve our understanding of the metastatic potential of salivary carcinomas. Key words: lymph vessels, salivary carcinoma, VEGF-C, HGF.Nodal metastasis is an important prognostic predictor in head and neck cancers, including salivary carcinomas. The risk for lymph node metastasis in these carcinomas is highly variable (9% -85%) [1] and strongly associated with the tumor histologic type [1][2][3][4][5]. It is a general consensus that high-grade mucoepidermoid carcinomas, salivary duct carcinomas, undifferentiated carcinomas and squamous cell carcinomas are high-risk tumors for nodal metastasis [1,[3][4][5]. In these the risk for neck metastasis has been described to be >50% [1] and, thus, elective neck dissection is considered in their management [1][2][3].Lymph vessels provide the main avenue for nodal metastasis and in head and neck squamous cell carcinoma (HNSCC) it has been shown that tumors of different anatomic regions do not vary significantly in their lymphangiogenic properties [6,7]. However, a high lymph vessel density (LVD) seems to be an indicator of the risk of lymph node metastasis in HNSCC [6][7][8][9]. Regarding salivary gland tumors, the lymphatic vessels have rarely been studied [10][11][12] and in a particular type of salivary carcinoma, i.e. in those arising in pleomorphic adenomas (CXPA) the lymphatic network was found to be composed mainly of pre-existing ...

show abstract

“…The correlation between LMVD and poor prognosis of tumor patients has been identified in breast cancer, prostate adenocarcinoma, colon cancer, melanoma, NSCLC and oral squamous cell carcinoma (20)(21)(22)(23)(24). However, the correlation between LMVD status (intratumoral or peritumoral) and tumor prognosis has not yet been fully clarified.…”

Section: Discussionmentioning

confidence: 99%

Peritumoral lymphatic microvessel density is related to poor prognosis in lung adenocarcinoma: A retrospective study of 65 cases

Zhang

Guan

Zhang

et al. 2012

Experimental and Therapeutic Medicine

View full text Add to dashboard Cite

Abstract. Although recent investigations have identified that lymphangiogenesis is associated with regional lymph node metastasis and tumor prognosis in non-small cell lung cancer (NSCLC), peritumoral lymphatic microvessel density (LMVD) and its prognostic significance in lung adenocarcinoma remain unknown. In the present study, we assessed peritumoral LMVD in lung adenocarcinoma and investigated its correlation with patient prognosis. Using immunohistochemistry (SP method), the D2-40-positive peritumoral LMVD count in lung adenocarcinoma was found to be 11.56±10.73, which was higher than intratumoral LMVD (P<0.001), and was found to be associated with lymphatic metastasis (P=0.003) and pTNM staging (P=0.046). Furthermore, a significant difference in the patient overall survival time was demonstrated between tumors with a high peritumoral LMVD and those with a low peritumoral LMVD (P=0.005). Finally, using multivariate analysis, it was determined that peritumoral LMVD, lymphatic metastasis and pTNM staging were independent prognostic factors. In conclusion, the results suggest that D2-40-positive peritumoral LMVD may predict the prognosis of lung adenocarcinoma.

show abstract

Lymphatic Microvessel Density and the Expression of Lymphangiogenic Factors in Oral Squamous Cell Carcinoma

Cited by 18 publications

References 59 publications

VEGF: A critical driver for angiogenesis and subsequent tumor growth: An IHC study

VEGF: A critical driver for angiogenesis and subsequent tumor growth: An IHC study

Lymphatic vessel density and expressions of lymphangiogenic growth factors in salivary carcinomas

Peritumoral lymphatic microvessel density is related to poor prognosis in lung adenocarcinoma: A retrospective study of 65 cases

Contact Info

Product

Resources

About