Lymph node metastasis in lower lip squamous cell carcinoma in relation to tumour size, histologic variables and p27Kip1 protein expression

Abstract

We studied a consecutive series of 95 patients undergoing radical surgical resection of lower lip squamous cell carcinoma (LLSCC) to assess the correlation between lymph node status and several prognostic variables, such as sex and age, tumour size, histologic grading, maximal microscopic tumour thickness, perineural infiltration and p27Kip1 protein status, to see which of these might be predictive of the development of lymph node metastases. Statistical analysis demonstrated a significant association between node status and tumour size, histological grading, maximal thickness, perineural invasion and p27Kip1 protein expression; additionally to node metastasis, low p27Kip1 protein expression was significant correlated with high microscopic thickness. These results indicate that lower lip squamous cell carcinomas of >2 cm, with G3-G4 histological grading, maximal thickness of >6 mm, perineural invasion and low p27Kip1 protein expression (LI <19.7%) are at high risk for the development of lymph node metastases

A clinicopathological study of perineural invasion and vascular invasion in oral tongue squamous cell carcinoma

“…In conclusion, perineural and vascular invasion are risk factors for regional metastasis 21 and adverse prognosis. In particular, perineural invasion has a strong relationship with 22 prognosis.…”

“…(OTSCC) patients [8][9][10][11][12][13][14][15][16][17][18][19][20][21][22] . However, the contribution of perineural and vascular invasions to 3 prognosis remains unclear because of contradictory reports.…”

Oral squamous cell carcinoma: Review of prognostic and predictive factors

“…Perineural invasion apparently correlates with higher probability of regional and distant metastases, higher depth of tumor invasion, lower differentiation, and lower 5-year survival rates in OSCC. 38 Rodolico et al 39 showed that perineural invasion correlates with the risk of nodal metastases.…”

Overexpression of cyclin D1 and interaction between p27Kip1 and tumour thickness predict lymph node metastases occurrence in lower lip squamous cell carcinoma

“…16 Briefly, the sections were reacted consecutively with monoclonal antibody K25020 (Transduction laboratories, Lexington, KY, USA) generated from mouse p27 (Kip1) protein at a concentration of 1:1.200 or, to detect cyclin D1, with mouse monoclonal antibody DCS-6 (DAKO, Glostrup, Denmark) at a dilution of 1:50 at 4°C overnight.…”

“…The measurement of microscopic thickness was performed with a 2.5 magnifying lens by an image analysis system (Leica QM500, Cambridge, UK) and the resulting software processing was expressed in mm as previously described. 16 …”