Lycopene inhibits reactive oxygen species production in SK-Hep-1 cells and attenuates acetaminophen-induced liver injury in C57BL/6 mice

Bandeira, Ana Carla Balthar; Silva, Talita Prato da; Araújo, Glaucy Rodrigues de; Araújo, Carolina Morais; Silva, Rafaella Cecília da; Lima, Wanderson Geraldo de; Bezerra, Frank Silva; Costa, Daniela Caldeira

doi:10.1016/j.cbi.2016.12.011

Cited by 22 publications

(15 citation statements)

References 66 publications

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“…An overproduction of ROS subsequently increases the oxidative stress and leads to a disequilibrium between oxidative stress and level of antioxidants. ROS, lipid peroxidation, and mitochondrial dysfunction lead to nuclear DNA fragmentation in succession and the cells eventually die [30, 31]. In this study, we found that the levels of antioxidants (SOD, GSH, and GSH-px) were increased while oxidative stress factors (MDA, 3-NT, and i-NOS) were lowered by MRS, indicating its antioxidant property.…”

Section: Discussionmentioning

confidence: 65%

Methane Alleviates Acetaminophen-Induced Liver Injury by Inhibiting Inflammation, Oxidative Stress, Endoplasmic Reticulum Stress, and Apoptosis through the Nrf2/HO-1/NQO1 Signaling Pathway

Yang

Cui

et al. 2019

Oxidative Medicine and Cellular Longevity

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Acetaminophen- (APAP-) induced hepatic injury is an important clinical challenge. Oxidative stress, inflammation, apoptosis, and endoplasmic reticulum stress (ERS) contribute to the pathogenesis. Methane has potential anti-inflammatory, antioxidant, and antiapoptotic properties. This project was aimed at studying the protective effects and relative mechanisms of methane in APAP-induced liver injury. In the in vivo experiment, C57BL/6 mice were treated with APAP (400 mg/kg) to induce hepatic injury followed by methane-rich saline (MRS) 10 ml/kg i.p. after 12 and 24 h. We observed that MRS alleviated the histopathological lesions in the liver, decreased serum aminotransferase levels, reduced the levels of inflammatory cytokines, suppressed the nuclear factor-κB expression. Further, we found that MRS relieved oxidative stress by regulating the Nrf2/HO-1/NQO1 signaling pathway and their downstream products after APAP challenge. MRS also regulated proteins associated with ERS-induced apoptosis. In the in vitro experiment, the L-02 cell line was treated with APAP (10 mM) to induce hepatic injury. We found that a methane-rich medium decreased the levels of reactive oxygen species (DHE fluorescent staining), inhibited apoptosis (cell flow test), and regulated the Nrf2/HO-1/NQO1 signaling pathway. Our data indicated that MRS prevented APAP-induced hepatic injury via anti-inflammatory, antioxidant, anti-ERS, and antiapoptotic properties involving the Nrf2/HO-1/NQO1 signaling pathway.

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Section: Discussionmentioning

confidence: 65%

Methane Alleviates Acetaminophen-Induced Liver Injury by Inhibiting Inflammation, Oxidative Stress, Endoplasmic Reticulum Stress, and Apoptosis through the Nrf2/HO-1/NQO1 Signaling Pathway

Yang

Cui

et al. 2019

Oxidative Medicine and Cellular Longevity

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“…Lycopene was also found to be effective against hepatotoxicity by acting as an antioxidant, regulating total glutathione (tGSH) and CAT concentrations, reducing glutathione disulfide (GSSG) and oxidative damage via reducing protein carbonylation. It also elevated MMP-2 down-regulation [110]. Regarding regulator of calcineurin 1 (RCAN1), lycopene reduced mitochondrial ROS and intracellular concentrations, NF-κB activity, Nucling expression, while decreasing the respiration per mitochondrion, MMP and glycolytic activity in RCAN1-overexpressing cells.…”

Section: Antioxidativementioning

confidence: 99%

Lycopene as a Natural Antioxidant Used to Prevent Human Health Disorders

et al. 2020

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Lycopene, belonging to the carotenoids, is a tetraterpene compound abundantly found in tomato and tomato-based products. It is fundamentally recognized as a potent antioxidant and a non-pro-vitamin A carotenoid. Lycopene has been found to be efficient in ameliorating cancer insurgences, diabetes mellitus, cardiac complications, oxidative stress-mediated malfunctions, inflammatory events, skin and bone diseases, hepatic, neural and reproductive disorders. This review summarizes information regarding its sources and uses amongst different societies, its biochemistry aspects, and the potential utilization of lycopene and possible mechanisms involved in alleviating the abovementioned disorders. Furthermore, future directions with the possible use of this nutraceutical against lifestyle-related disorders are emphasized. Its protective effects against recommended doses of toxic agents and toxicity and safety are also discussed.

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“…In vitro and in vivo studies demonstrated that lycopene reduces ROS production in SK-Hep-1 cells by inhibiting dicotinamide adenine dinucleotide phosphate oxidase (NADPH) through protein kinase C (PKC) signaling. Furthermore, it was indicated that lycopene improved hepatotoxicity by acting as an antioxidant, mainly by reducing protein carbonylation and areas of necrosis, ameliorating the general appearance of the lesion in C57BL/6 mice [146]. A recent study showed that lycopene exerted anti-inflammatory activities against paracetamol liver injury (APAP) in C57BL/6 mice by improving the redox state [147].…”

Section: Carotenoids and Hepatic Healthmentioning

confidence: 99%

Nutritional Importance of Carotenoids and Their Effect on Liver Health: A Review

2019

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The consumption of carotenoids has beneficial effects on health, reducing the risk of certain forms of cancer, cardiovascular diseases, and macular degeneration, among others. The mechanism of action of carotenoids has not been clearly identified; however, it has been associated with the antioxidant capacity of carotenoids, which acts against reactive oxygen species and inactivating free radicals, although it has also been shown that carotenoids modulate gene expression. Dietary carotenoids are absorbed and accumulated in the liver and other organs, where they exert their beneficial effects. In recent years, it has been described that the intake of carotenoids can significantly reduce the risk of suffering from liver diseases, such as non-alcoholic fatty liver disease (NAFLD). This disease is characterized by an imbalance in lipid metabolism producing the accumulation of fat in the hepatocyte, leading to lipoperoxidation, followed by oxidative stress and inflammation. In the first phases, the main treatment of NAFLD is to change the lifestyle, including dietary habits. In this sense, carotenoids have been shown to have a hepatoprotective effect due to their ability to reduce oxidative stress and regulate the lipid metabolism of hepatocytes by modulating certain genes. The objective of this review was to provide a description of the effects of dietary carotenoids from fruits and vegetables on liver health.

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Lycopene inhibits reactive oxygen species production in SK-Hep-1 cells and attenuates acetaminophen-induced liver injury in C57BL/6 mice

Cited by 22 publications

References 66 publications

Methane Alleviates Acetaminophen-Induced Liver Injury by Inhibiting Inflammation, Oxidative Stress, Endoplasmic Reticulum Stress, and Apoptosis through the Nrf2/HO-1/NQO1 Signaling Pathway

Methane Alleviates Acetaminophen-Induced Liver Injury by Inhibiting Inflammation, Oxidative Stress, Endoplasmic Reticulum Stress, and Apoptosis through the Nrf2/HO-1/NQO1 Signaling Pathway

Lycopene as a Natural Antioxidant Used to Prevent Human Health Disorders

Nutritional Importance of Carotenoids and Their Effect on Liver Health: A Review

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