Lycopene as a natural protector against γ-radiation induced DNA damage, lipid peroxidation and antioxidant status in primary culture of isolated rat hepatocytes in vitro

Srinivasan, M.; Sudheer, Adluri Ram; Pillai, Kamala; Kumar, Pankaj; Sudhakaran, P. R.; Menon, Venugopal P.

doi:10.1016/j.bbagen.2006.11.008

Cited by 99 publications

(72 citation statements)

References 34 publications

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“…The results coincide with earlier reports and it appears that measurable DNA damage is present in case of cytotoxic hepatocyte injury induced by D-GalN/LPS. Numerous studies reveal the ability of lycopene to protect against DNA damage induced by toxic substances (Srinivasan et al, 2007). In this study, lycopene pretreatment stabilized the hepatocytes from DNA damage indicating its protective nature against D-GalN/ LPS-induced genotoxicity and endorses the earlier findings.…”

Section: Discussionsupporting

confidence: 72%

“…The carbon centered radical reacts readily and reversibly with oxygen to form a new chain carrying peroxyl radicals, which are highly stable forms than ROS and thereby inhibiting lipid peroxidation (Aggarwal et al, 2009). The free radical scavenging effect of lycopene may be attributed by its potent antioxidant property (Srinivasan et al, 2007). The primary defenses against oxidative stress are exerted by the resident antioxidants in the tissue.…”

Section: Discussionmentioning

confidence: 99%

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Effect of lycopene on oxidative stress induced duringd-galactosamine/lipopolysaccharide-sensitized liver injury in rats

Abdulazeez

Devaki

2013

Pharmaceutical Biology

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Context: Lycopene is a phytonutrient under considerable investigation for its antioxidant benefits in treating diseases like cancer, cardiovascular diseases, osteoporosis and diabetes. Objective: This study explores the effect of lycopene against oxidative damage during experimental hepatitis, induced by D-galactosamine/lipopolysaccharide (D-GalN/LPS). Materials and methods: Experimental rats were pretreated with lycopene intraperitoneally for 6 d (10 mg/kg body weight/day) and then induced by D-GalN/LPS. After induction, the levels of lipid peroxides in serum and liver of control and experimental group of animals were measured. The activities of enzymatic antioxidants such as superoxide dismutase, catalase, glutathione peroxidase and glutathione S-transferase and nonenzymatic antioxidants, such as reduced glutathione, vitamin C and vitamin E were also analyzed. The genotoxic effect of D-GalN/LPS was evaluated through the comet assay. Results: The elevated level of lipid peroxides induced by D-GalN/LPS was significantly (p50.05) reverted in lycopene pretreated animals. Lycopene administration restored (p50.05) the decreased activities of enzymatic and nonenzymatic antioxidant markers during D-GalN/LPS induction. The DNA strand breaks (72.3 mM) generated during D-GalN/LPS toxic injury was significantly reduced (35.5 mM) upon pretreatment with lycopene as observed by reduced tail movement in comet assay. Discussion and conclusion: There is no conclusive report about lycopene-assisted protection against free radical mediated toxic injury induced by D-GalN/LPS. Our findings reveal that lycopene effectively combated oxidative damage and protected antioxidant defense status of the cell. Pretreatment of lycopene also offers protection against the DNA damage and confirms the antioxidant nature of the phytonutreint against experimental hepatitis.

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Section: Discussionsupporting

confidence: 72%

Section: Discussionmentioning

confidence: 99%

Effect of lycopene on oxidative stress induced duringd-galactosamine/lipopolysaccharide-sensitized liver injury in rats

Abdulazeez

Devaki

2013

Pharmaceutical Biology

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“…Pretreatment of lycopene resulted in increased levels of these antioxidants. These may be attributed to lycopene's ability to act as an antioxidant and singlet oxygen quencher [58].…”

Section: Discussionmentioning

confidence: 99%

Variations of Plant Macronutrients and Secondary Metabolites Content in Response to Radionuclides Accumulation

Hegazy¹,

Sl²,

Hf³

et al. 2013

J Bioremed Biodeg

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Current knowledge on the basic radionuclide tolerance indicates that plants may develop mechanisms preventing the damaging effects of radionuclide stress. The radionuclides content was determined in the Mediterranean coastal black sand soil and in the edible portions of the four food crop plant species, viz., Eruca sativa, Lycopersicon esculentum, Pasidium guajava and Mangifera indica. Biochemical and nutritional constituents were assessed to elucidate the possible effect of radionuclides on the edible portions of the plants. The ability of the four study plant species to accumulate Uranium and Thorium in their edible portions from the coastal black sand soil is higher than that absorbed from the inland agricultural soils. The nutritional constituents were affected by the high concentrations of Uranium and Thorium in the plant tissues. The total protein, moisture content, total phenolic compounds, total chlorophyll, total carotenoids, β-Carotene and Lycopene were positively correlated with Uranium and Thorium concentrations in the plant tissues, while the mineral content, vitamin C and total flavonoids were suppressed by high concentrations of Uranium and Thorium in the plant tissues. The fluctuations in the biochemical or nutritional constituents of the plant edible portions are considered an important defense mechanism to control the redox state of the cells accumulating radionuclides with high levels. Journal of Bior emediation & Biodegradation

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“…Referring to study done by Wilcox (2003), Basu and Imrhan (2007), and Srinivasan (2007), tomatoes contain lycopene and is believed to prevent many diseases due to its high antioxidant content [18][19][20] . Lycopene is the most dominant antioxidant in tomatoes that possess activities such as antimicrobial, antithrombogenik, antivirus, reduce blood cholesterol and inhibit cell proliferation.…”

Section: Discussionmentioning

confidence: 99%

“…Activity of lycopene is cosidered two-folds higher than β-caroten and ten-folds higher than α-karoten 24 . In vitro study reported that lycopene gives protetection on lipoprotein of cell membrane, DNA and vasculer against oxidant [18][19][20] . In summary, lycopene possess high antioxidant and antiangiogenesis that plays role as precursor in scavenging reactive oxygen and reduce free radicals that recover trophoblast cells induced by preeclampsia as indicated by decrease in MDA level.…”

Section: Discussionmentioning

confidence: 99%

Effect of Lycopene on Level of Malondialdehid (MDA) in Preeclampsia-Induced Placental Trophoblast Cells

Basyir¹,

Fauziah²,

Yanwirasti³

et al. 2017

IJPTR

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: Preeclampsia is a mjor cause in both maternal and perinatal mortality and morbidity.Underlying mechanism of preeclampsia remains unclear. It is assumed that preeclampsia is caused by imbalance in free radicals and antioxidant in blood and placenta. Lycopene, known to possess antioxidant properties, is therefore a promising agent to decrease preeclampsia risk. This study aimed to observe lycopene on MDA level in placental trophoblast which is induced by preeclampsia in vitro. Level of MDA was measured with TBARS (thiobarbituric acid-reactive substances). In preeclampsiainduced trophoblast, MDA level significantly reduced (p<0,001) from 18,8923 μM to 8,5773 μM after treated with lycopene of 31,25 μg/ml incubated for 24 hours, and from 18,899 μM to 8,6671 μM after incubation for 48 hours. Lycopene possess high antioxidant and antiangiogenesis that plays role as precursor in scavenging reactive oxygen and reduce free radicals that recover trophoblast cells induced by preeclampsia as indicated by decrease in MDA level. Further studies regarding the optimal concentration of lycopene on embryo cell for clinical trial, are encouraged.

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Lycopene as a natural protector against γ-radiation induced DNA damage, lipid peroxidation and antioxidant status in primary culture of isolated rat hepatocytes in vitro

Cited by 99 publications

References 34 publications

Effect of lycopene on oxidative stress induced duringd-galactosamine/lipopolysaccharide-sensitized liver injury in rats

Effect of lycopene on oxidative stress induced duringd-galactosamine/lipopolysaccharide-sensitized liver injury in rats

Variations of Plant Macronutrients and Secondary Metabolites Content in Response to Radionuclides Accumulation

Effect of Lycopene on Level of Malondialdehid (MDA) in Preeclampsia-Induced Placental Trophoblast Cells

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