Lycium barbarum polysaccharides improve lipid metabolism disorders of spotted sea bass Lateolabrax maculatus induced by high lipid diet

Huang, Zhangfan; Ye, Youling; Long, Zhongying; Qin, Huihui; Liu, Longhui; Xu, Anle; Li, Zhongbao

doi:10.1016/j.ijbiomac.2023.125122

Cited by 11 publications

(5 citation statements)

References 75 publications

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“…The molecular weight and yield of composition 2 were 35.508 kDa and 20.9%, respectively, and including glucose (96.59%), galactose (1.01%), arabinose (1.69%), mannose (0.44%), and xylose (0.27%). More detailed results on the preparation and characterization of LBP can be found in previous report (21).…”

Section: Lbp Preparation and Characterizationmentioning

confidence: 99%

“…Many pharmacological and phytochemical studies have shown that LBP has antioxidant ( 16 ), immunomodulatory ( 17 ), hypoglycemic ( 18 ), anti-inflammatory ( 19 ) and liver protection ( 20 ). Meanwhile, in our previous study, LBP was found to have excellent effects in regulating hepatic lipid metabolism ( 21 ). Thus, LBP may be able to mitigate the adverse effects of HSBMD on the body through the aforementioned effects.…”

Section: Introductionmentioning

confidence: 97%

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Effects of Lycium barbarum polysaccharides supplemented to high soybean meal diet on immunity and hepatic health of spotted sea bass Lateolabrax maculatus

Liu,

Zhao,

Huang

et al. 2024

Front. Immunol.

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High soybean meal diet (HSBMD) decreased the immunity and damaged the liver health of spotted sea bass; in this study, Lycium barbarum polysaccharides (LBP) was added to HSBMD to explore its effects on the immunity and liver health. The diet with 44% fish meal content was designed as a blank control. On this basis, soybean meal was used to replace 50% fish meal as HSBMD, and LBP was added in HSBMD in gradient (1.0, 1.5, 2.0 g/kg) as the experimental diet. 225-tailed spotted sea bass with initial body weight of 44.52 ± 0.24 g were randomly divided into 5 groups and fed the corresponding diet for 52 days, respectively. The results show that: after ingestion of HSBMD, the immunity of spotted sea bass decreased slightly and hepatic tissue was severely damaged. And the addition of LBP significantly improved the immune capacity and protected the hepatic health. Specifically, the activities of serum lysozyme (LZM), immunoglobulin M (IgM), liver acid phosphatase (ACP) and alkaline phosphatase (AKP) were increased, and serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities were significantly decreased, and hepatic morphology was improved. In the analysis of transcriptome results, it was found that toll-like receptor 3 (TLR3) and toll-like receptor 5 (TLR5) were down-regulated in toll-like receptor signaling pathway. And LBP may protect hepatic health by regulating Glycolysis/Gluconeogenesis, Insulin signaling pathway, Steroid biosynthesis and other glucolipid-related pathways. In conclusion, the addition of LBP in HSBMD can improve the immunity and protect the hepatic health of spotted sea bass, and its mechanism may be related to glucose and lipid metabolism.

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Section: Lbp Preparation and Characterizationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

Effects of Lycium barbarum polysaccharides supplemented to high soybean meal diet on immunity and hepatic health of spotted sea bass Lateolabrax maculatus

Liu,

Zhao,

Huang

et al. 2024

Front. Immunol.

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“…Lipid metabolism, including lipid synthesis, transport, and breakdown, is essential for the maintenance of body homeostasis . Lipid metabolism disorder can cause a series of pathological changes, such as hyperlipidemia, atherosclerosis, obesity, fatty liver, etc., and is even closely related to the occurrence and progression of cancer. − The 3T3-L1 preadipocyte cell line is a classical model for studying lipid metabolism, which can be induced to differentiate into typical white adipocytes. During this process, the key regulatory genes include sterol regulatory element binding protein 1c (SREBP1c), peroxide proliferation-activating receptor γ (PPARγ), CCAAT enhancer binding protein α (C/EBPα), etc. , They are all located intracellularly and can be regulated by many substances directly or indirectly. − The main action mechanisms include the interaction of compound with membrane receptors or intracellular target proteins .…”

Section: Introductionmentioning

confidence: 99%

In Vitro Comparative Analysis of the Effect and Structure-Based Influencing Factors of Flavonols on Lipid Accumulation

Ang,

Yang,

Wang

et al. 2024

J. Agric. Food Chem.

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Flavonols represented by quercetin have been widely reported to have biological activities of regulating lipid metabolism. However, the differences in flavonols with different structures in lipid-lowering activity and the influencing factors remain unclear. In this study, the stability, transmembrane uptake ratio, and lipid metabolism regulation activities of 12 flavonol compounds in the 3T3-L1 cell model were systematically compared. The results showed that kaempferide had the highest cellular uptake ratio and the most potent inhibitory effect on adipogenesis at a dosing concentration of 20 μM, followed by isorhamnetin and kaempferol. They inhibited TG accumulation by more than 65% and downregulated the expression of PPARγ and SREBP1c by more than 60%. The other four aglycones, including quercetin, did not exhibit significant activity due to the structural instability in the cell culture medium. Meanwhile, five quercetin glucosides were quite stable but showed a low uptake ratio that no obvious activity was observed. Correlation analysis also showed that for 11 compounds except galangin, the activity was positively correlated with the cellular uptake ratio (p < 0.05, r = 0.6349). These findings may provide a valuable idea and insight for exploring the structure-based activity of flavonoids at the cellular level.

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“…The fruit of L. barbarum exhibits a diverse array of biological activities, encompassing antioxidation, reduction of blood sugar and blood lipid levels, anti-cancer properties, anti-aging effects, alleviation of sore waist and knees, and neuroprotection ( Huang et al, 2023 ; Li et al, 2023 ; Wang et al, 2023 ; Yang et al, 2023 ; Zheng et al, 2023 ). Modern research has unveiled that L. barbarum is rich in various chemical components, such as flavonoids, alkaloids, phenolic acids, lipids, polysaccharides, amino acids, and carotenoids ( Fakhfakh et al, 2022 ; Chen et al, 2023 ; Liu et al, 2023 ; Ma et al, 2023 ).…”

Section: Introductionmentioning

confidence: 99%

Exploration of chemical compositions in different germplasm wolfberry using UPLC-MS/MS and evaluation of the in vitro anti-inflammatory activity of quercetin

Lan,

Duan,

et al. 2024

Front. Pharmacol.

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Wolfberry, esteemed as a traditional Chinese medicinal material and functional food, is replete with nutrients and boasts a diverse array of health benefits, including hypoglycemic, antitumor, antioxidant, anti-inflammatory, and immune-enhancing properties. Notably, inflammation is a pivotal factor in the onset and progression of numerous diseases. Despite this, there is a paucity of research on the comprehensive evaluation of the components found in different wolfberries, and the exploration of their primary active components is limited. To address this issue, we conducted a comprehensive targeted metabolomics analysis, employing statistical methods such as principal component analysis (PCA), orthogonal partial least squares discriminant analysis (OPLS-DA), KEGG pathway analysis, and volcano plots to delineate the compositional differences among red, black, and yellow wolfberries. Furthermore, we investigated the anti-inflammatory effects of their primary components through in vitro experiments. Our analysis revealed a total of 1,104 chemical compositions in the three wolfberries, with alkaloids, phenolic acids, flavonoids, and lipids being the predominant nutritional components. KEGG enrichment analysis indicated that these compositions were primarily involved in the biosynthesis of secondary metabolites, ABC transport, and galactose metabolism pathway. Moreover, our study demonstrated that quercetin exhibited dose-dependent anti-inflammatory activity in LPS-stimulated HUVECs. It effectively inhibited the production of inflammatory factors such as TNF-α, MCP-1, and IL-1β, while also down-regulating the gene and protein expression levels of ICAM-1 and VCAM-1. In conclusion, our findings indicate that there are variations in compositions among the three wolfberries, with flavonoids being the most abundant, and in vitro studies also confirmed the anti-inflammatory potential of quercetin. It is worth noting that Lycium ruthenicum contains higher levels of antioxidant components and possesses greater nutritional value, providing valuable insights for the future development and utilization of the three wolfberries.

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Lycium barbarum polysaccharides improve lipid metabolism disorders of spotted sea bass Lateolabrax maculatus induced by high lipid diet

Cited by 11 publications

References 75 publications

Effects of Lycium barbarum polysaccharides supplemented to high soybean meal diet on immunity and hepatic health of spotted sea bass Lateolabrax maculatus

Effects of Lycium barbarum polysaccharides supplemented to high soybean meal diet on immunity and hepatic health of spotted sea bass Lateolabrax maculatus

In Vitro Comparative Analysis of the Effect and Structure-Based Influencing Factors of Flavonols on Lipid Accumulation

Exploration of chemical compositions in different germplasm wolfberry using UPLC-MS/MS and evaluation of the in vitro anti-inflammatory activity of quercetin

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