Lycium barbarum Polysaccharide Ameliorates Heat-Stress-Induced Impairment of Primary Sertoli Cells and the Blood-Testis Barrier in Rat via Androgen Receptor and Akt Phosphorylation

Hu, Su-Qin; Liu, Dian-long; Liu, Sijia; Li, Chunrui; Guo, Jian

doi:10.1155/2021/5574202

Cited by 12 publications

(11 citation statements)

References 77 publications

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“…Increased apoptosis may have negative consequences on sperm production, ultimately jeopardizing male fertility ( Zhang et al, 2019 ). Some antioxidant-rich natural substances, such as morin ( Arisha et al, 2019 ), luteolin, and Lycium barbarum polysaccharide ( Hu et al, 2021 ), also have anti-apoptotic properties, minimizing damage to the BTB. Antioxidant supplementation may be a potentially viable treatment method for both the prevention and relief of BTB damage, according to evidence from these animal investigations.…”

Section: Discussionmentioning

confidence: 99%

Bibliometric and visual analysis of blood-testis barrier research

et al. 2022

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Background: Extensive research on the blood-testis barrier has been undertaken in recent years. However, no systematic bibliometric study has been conducted on this subject. Our research aimed to identify the hotspots and frontiers of blood-testis barrier research and to serve as a guide for future scientific research and decision-making in the field.Methods: Studies on the blood-testis barrier were found in the Web of Science Core Collection. VOSviewer, CiteSpace, and Microsoft Excel were used to conduct the bibliometric and visual analyses.Results: We found 942 blood-testis barrier studies published in English between 1992 and 2022. The number of annual publications and citations increased significantly between 2011 and 2022, notably in the United States. China and the United States, the US Population Council, Endocrinology, and Cheng C. Yan were the most productive countries, institution, journal, and author, respectively. The study keywords indicated that blood-testis barrier research involves a variety of compositional features (tight junctions, cytoskeleton, adherens junctions), cell types (Sertoli cells, germ cells, Leydig cells, stem cells), reproductive toxicity (cadmium, nanoparticles, bisphenol-a), and relevant mechanisms (spermatogenesis, apoptosis, oxidative stress, dynamics, inflammation, immune privilege).Conclusion: The composition and molecular processes of the blood-testis barrier as well as the blood-testis barrier in male infertility patients are the primary research hotspots in this field. In addition, future research will likely focus on treatment and the development of novel medications that target signal pathways in oxidative stress and apoptosis to preserve the blood-testis barrier. Further studies must extend to clinical diagnosis and therapy.

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Section: Discussionmentioning

confidence: 99%

Bibliometric and visual analysis of blood-testis barrier research

et al. 2022

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“…In the study of HaCaT cell injury induced by PM2.5, it was found that LBP could reduce apoptosis, inhibit the expression of GRP78 and CHOP, reduce the transformation of LC3I to LC3II, inhibit Bax protein, and activate Bcl-2 protein. 63 In this study, the apoptosis rate of testes and epididymis in 2,4-D + LBP group was lower than that in 2,4-D group, and the expression of apoptosis-related genes in testicular tissue of 2,4-D + LBP group was lower than that in 2,4-D group. These results suggest that LBP can reduce the apoptosis of testicular cells induced by 2,4-D, and its mechanism may be related to the inhibition of Fas/FasL signal pathway.…”

Section: Discussionmentioning

confidence: 45%

“…It has been found that LBP can reduce the expression of HIF1‐α and Bcl2 in human trophoblast cells exposed to H 2 O 2 , and reduce the expression of Bax, thus inhibiting apoptosis and oxidative stress. In the study of HaCaT cell injury induced by PM2.5, it was found that LBP could reduce apoptosis, inhibit the expression of GRP78 and CHOP, reduce the transformation of LC3I to LC3II, inhibit Bax protein, and activate Bcl‐2 protein 63 . In this study, the apoptosis rate of testes and epididymis in 2,4‐D + LBP group was lower than that in 2,4‐D group, and the expression of apoptosis‐related genes in testicular tissue of 2,4‐D + LBP group was lower than that in 2,4‐D group.…”

Section: Discussionmentioning

confidence: 99%

Mechanism of 2,4‐Dichlorophenoxyacetic acid‐induced damage to rat testis via Fas/FasL pathway and the protective effect of Lycium barbarum polysaccharides

Zhou

Wang

et al. 2022

Environmental Toxicology

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The herbicide 2,4-Dichlorophenoxyacetic acid (2,4-D) is widely used to control broadleaved weeds and has been associated with male infertility. We studied the molecular mechanisms of 2,4-D induced male reproductive system damage and the protective effects of Lycium barbarum polysaccharides (LBP) using Sprague Dawley rats and TM4 cells. Treatment with 2,4-D caused architectural and functional changes in the testis, including collapsed and atrophied seminiferous tubules with reduced number of spermatozoa, scarce sperm in the epididymal duct, low levels of serum testosterone, decreased superoxide dismutase and glutathione peroxidase activity, high malondialdehyde content, and increased apoptosis in the testis and epididymis. The expression of Fas, FasL, FADD, Pro-caspase-8, Cleaved-Caspase-8, Pro-Caspase-3, and Cleaved-Caspase-3 were significantly increased in the testicular tissue of 2,4-D-treated rats. The proliferative activity of TM4 cells decreased with an increase in dose and time of 2,4-D exposure, along with enhanced Fas/Fas ligand expression and a decreased concentration of inhibin B in TM4 cell culture medium. Depletion of Fas by specific shRNA transfection reversed the effects of 2,4-D in TM4 cells, further confirming the involvement of death receptor pathway in 2,4-Dmediated apoptosis of sertoli cells. Treatment with LBP also reversed the effects of 2,4-D in testicular cells, resulting in improved cell architecture along with enhanced proliferative capacity. Moreover, in response to LBP treatment of Sertoli cells, the content of inhibin B increased, the level of reactive oxygen species and malondialdehyde decreased, the activities of superoxide dismutase and glutathione peroxidase increased, and the rate of apoptosis as well as the expression of Fas/Fas ligand signaling pathway proteins decreased.

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“…SCs and BTB may not be able to provide su cient nutrients and a safe environment for spermatogenesis if their structure or function was destroyed, resulting in abnormal spermatogenesis or survival, leading to infertility. High temperature is a common cause of structural and functional damage to SCs and BTB, and a 43°C water bath is usually used to establish a testicular heat stress model [23][24].…”

Section: Discussionmentioning

confidence: 99%

Integrating network pharmacology and in vitro experiment to investigate the protective effects of semen cuscutae flavonoid on sertoli cells and blood-testis barrier in male infertility under heat stress

Liu

et al. 2022

Preprint

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Background: Semen cuscutae is one of the common drugs used in treating male infertility (MI) in Traditional Chinese Medicine (TCM), and semen cuscutae flavonoid (SCF) is the main active component of semen cuscutae. The mechanism of SCF on sertoli cells (SCs) and blood-testis barrier (BTB) in male infertility under heat stress have not been clarified yet. Methods: Here, network pharmacology were used to explore the targets and signaling pathways of SCF in the treatment of MI. Then, in vitro experiments were integrated to verify the results of network pharmacology. In the in vitro experiments, the cell viability was measured by performing the 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, Ki67 and AR expression were detected by performing immunohistochemistry staining, and AR, Akt, CK-18, and Occludin levels were measured using western blotting. The Akt inhibitor assay was further performed to elucidate the signaling pathway underlying the function of SCF.Result: The data showed a total of 136 potential therapeutic targets of SCF, including HPS90AA1, SRC, JUN, TP53, MAPK1, AKT1, etc., were identified. In vitro experiments suggested that SCF significantly improved the viability of SCs, upregulated the expression of AR, Akt, Occludin, and Ki67, and downregulated the expression of CK-18. After adding the Akt inhibitor to block the PI3K-Akt signaling pathway, the effect of SCF decreased, indicating that SCF regulates the PI3K-Akt signaling pathway via AR, and then regulates occludin, Ki67, and CK-18. Gene ontology (GO) enrichment and Kyoto Encyclopedia of genes and genomes (KEGG) pathway analyses also suggested that SCF regulates cell proliferation and differentiation mainly via the PI3K-Akt signaling pathway. Conclusions: We suggested that the active components of SCF can promote AR expression, and regulate the PI3K-Akt signaling pathway and its downstream factors (occludin, Ki67, and CK-18), to regulate the viability, proliferation, differentiation, and BTB permeability of SCs, ultimately protecting reproductive ability.

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Lycium barbarum Polysaccharide Ameliorates Heat-Stress-Induced Impairment of Primary Sertoli Cells and the Blood-Testis Barrier in Rat via Androgen Receptor and Akt Phosphorylation

Cited by 12 publications

References 77 publications

Bibliometric and visual analysis of blood-testis barrier research

Bibliometric and visual analysis of blood-testis barrier research

Mechanism of 2,4‐Dichlorophenoxyacetic acid‐induced damage to rat testis via Fas/FasL pathway and the protective effect of Lycium barbarum polysaccharides

Integrating network pharmacology and in vitro experiment to investigate the protective effects of semen cuscutae flavonoid on sertoli cells and blood-testis barrier in male infertility under heat stress

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