Lux Biosensors: Screening Biologically Active Compounds for Genotoxicity

Igonina, E. V.; Марсова, М. В.; Абилев, С. К.

doi:10.1134/s2079059718010082

Cited by 3 publications

(6 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It takes more time for PcolD to respond: 50 minutes-induction onset (T min ), 3.5 hours-maximum response (T max ). This is consistent with the data obtained earlier for other genotoxic substances and is explained by the time spent on entering into the cell, DNA damaging that blocks replication [31], formation of single strand ends and activation of SOS response [10,32]. The long time required for the PsoxS response (T min = 70 min, T max = 4 hours) is apparently explained by the fact that the appearance of superoxide anion radical in the cell is obviously associated only with damage of the respiratory chain [33].…”

Section: Plos Onesupporting

confidence: 92%

“…The test using E. coli MG1655 (pColD-lux) is more sensitive to genotoxic agents than SOS chromotest [34], which is used in toxicology along with the Ames test [35,36] to determine the rate of mutagenesis. These tests correlate well with each other [32], but may underestimate the effect of alkylating compounds on the rate of mutagenesis [37]. In the present work, using the E. coli MG1655 (pAlkA-lux) biosensor, it was shown that during incubation of cells with BBH, DNA alkylation is not observed and, obviously, alkylating compounds do not appear in the medium.…”

Section: Plos Onesupporting

confidence: 54%

See 1 more Smart Citation

Genotoxic effect of 2,2’-bis(bicyclo[2.2.1] heptane) on bacterial cells

et al. 2020

View full text Add to dashboard Cite

The toxic effect of strained hydrocarbon 2,2'—bis (bicyclo[2.2.1]heptane) (BBH) was studied using whole-cell bacterial lux-biosensors based on Escherichia coli cells in which luciferase genes are transcriptionally fused with stress-inducible promoters. It was shown that BBH has the genotoxic effect causing bacterial SOS response however no alkylating effect has been revealed. In addition to DNA damage, there is an oxidative effect causing the response of OxyR/S and SoxR/S regulons. The most sensitive to BBH lux-biosensor was E . coli pSoxS-lux which reacts to the appearance of superoxide anion radicals in the cell. It is assumed that the oxidation of BBH leads to the generation of reactive oxygen species, which provide the main contribution to the genotoxicity of this substance.

show abstract

Section: Plos Onesupporting

confidence: 92%

Section: Plos Onesupporting

confidence: 54%

Genotoxic effect of 2,2’-bis(bicyclo[2.2.1] heptane) on bacterial cells

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Lucerase catalyzes the oxidation of tetradecanal with the emission of a photon of blue-green light (λ max = 490 nm), which was detected by a luminometer. Integration time to record a response (significant bioluminescence increase) at such wavelength in the presence of H 2 O 2 was equal to 30 min, which is in accordance with the range reported in literature: 5–20 min [22,23].…”

Section: Methodssupporting

confidence: 89%

“…Bioluminescence induction factor I was calculated relative to the control cells using standard procedure, as described in [23,25,26], i.e., as I = (L e – L c )/L c , where L e – L c are luminescence intensities of the experimental and control specimens, respectively. Protective activity was calculated similarly as P = ( 1 - I f /I c ) × 100%, where I f and I c are induction factors of the solution with and without added fullerene, respectively.…”

Section: Methodsmentioning

confidence: 99%

Biological Effects of C60 Fullerene Revealed with Bacterial Biosensor—Toxic or Rather Antioxidant?

et al. 2019

View full text Add to dashboard Cite

Nanoparticles have been attracting growing interest for both their antioxidant and toxic effects. Their exact action on cells strongly depends on many factors, including experimental conditions, preparation, and solvents used, which have contributed to the confusion regarding their safety and possible health benefits. In order to clarify the biological effects of the most abundant fullerene C60, its impact on the Escherichia coli model has been studied. The main question was if C60 would have any antioxidant influence on the cell and, if yes, whether and to which extent it would be concentration-dependent. An oxidative stress induced by adding hydrogen peroxide was measured with an E. coli MG1655 pKatG-lux strain sensor, with its time evolution being recorded in the presence of fullerene C60 suspensions of different concentrations. Optimal conditions for the fullerene C60 solubilization in TWEEN 80 2% aqueous solution, together with resulting aggregate sizes, were determined. Results obtained for the bacterial model can be extrapolated on eukaryote mitochondria. The ability of C60 to penetrate through biological membranes, conduct protons, and interact with free radicals is likely responsible for its protective effect detected for E. coli. Thus, fullerene can be considered as a mitochondria-targeted antioxidant, worth further researching as a prospective component of novel medications.

show abstract

“…For testing chemical impurities (toxins) and other biologically active substances, primarily medications, luminescent biosensor cells (lux-biosensors) are currently used in two ways: (1) based on bioluminescence quenching [1][2][3], and (2) based on bioluminescence induction [4][5][6][7][8][9][10][11][12][13]. The existing lux-biosensors based on genetically engineered Escherichia coli cells and a number of naturally luminescent enterobacteria are of little use for working with soil pollution [14].…”

Section: Introductionmentioning

confidence: 99%

Constructing of Bacillus subtilis-Based Lux-Biosensors with the Use of Stress-Inducible Promoters

Kessenikh

Novoyatlova

Bazhenov

et al. 2021

IJMS

View full text Add to dashboard Cite

Here, we present a new lux-biosensor based on Bacillus subtilis for detecting of DNA-tropic and oxidative stress-causing agents. Hybrid plasmids pNK-DinC, pNK-AlkA, and pNK-MrgA have been constructed, in which the Photorhabdus luminescens reporter genes luxABCDE are transcribed from the stress-inducible promoters of B. subtilis: the SOS promoter PdinC, the methylation-specific response promoter PalkA, and the oxidative stress promoter PmrgA. The luminescence of B. subtilis-based biosensors specifically increases in response to the appearance in the environment of such common toxicants as mitomycin C, methyl methanesulfonate, and H2O2. Comparison with Escherichia coli-based lux-biosensors, where the promoters PdinI, PalkA, and Pdps were used, showed generally similar characteristics. However, for B. subtilis PdinC, a higher response amplitude was observed, and for B. subtilis PalkA, on the contrary, both the amplitude and the range of detectable toxicant concentrations were decreased. B. subtilis PdinC and B. subtilis PmrgA showed increased sensitivity to the genotoxic effects of the 2,2′-bis(bicyclo [2.2.1] heptane) compound, which is a promising propellant, compared to E. coli-based lux-biosensors. The obtained biosensors are applicable for detection of toxicants introduced into soil. Such bacillary biosensors can be used to study the differences in the mechanisms of toxicity against Gram-positive and Gram-negative bacteria.

show abstract

Lux Biosensors: Screening Biologically Active Compounds for Genotoxicity

Cited by 3 publications

References 32 publications

Genotoxic effect of 2,2’-bis(bicyclo[2.2.1] heptane) on bacterial cells

Genotoxic effect of 2,2’-bis(bicyclo[2.2.1] heptane) on bacterial cells

Biological Effects of C60 Fullerene Revealed with Bacterial Biosensor—Toxic or Rather Antioxidant?

Constructing of Bacillus subtilis-Based Lux-Biosensors with the Use of Stress-Inducible Promoters

Contact Info

Product

Resources

About