Genotoxic effect of 2,2’-bis(bicyclo[2.2.1] heptane) on bacterial cells

Kessenikh, A.; Gnuchikh, E. Yu.; Bazhenov, Sergey V.; Бермешев, М. В.; Pevgov, V. G.; Samoilov, Vadim O.; Shorunov, Sergey V.; Максимов, А. Л.; Yaguzhinsky, L. S.; Манухов, И. В.

doi:10.1371/journal.pone.0228525

Cited by 12 publications

(11 citation statements)

References 33 publications

(51 reference statements)

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“…According to the data obtained, the threshold concentration of BBH for B. subtilis 168 pNK-DinC is 0.1 g/L. For the E. coli MG1655 pDinI biosensor-1 g/L, the same as that for E. coli MG1655 pColD-lux biosensor, according the work [32]. The maximum response amplitude for B. subtilis 168 pNK-DinC was 50-fold, while for E. coli pDinI, it was only 4 (Figure 4C).…”

Section: Application Of the Obtained Lux-biosensors For Assessment Of Bbh Toxicity Against Gram-positive Bacteriasupporting

confidence: 52%

“…The threshold BBH concentration for B. subtilis 168 pNK-MrgA is between 0.1 and 1 g/L (Figure 5A). This is a higher sensitivity compared to E. coli pDps (1 g/L) (Figure 5B) and the E. coli pOxyR-lux biosensors (specific to H 2 O 2 , according to [32], its sensitivity to BBH is 1 g/L), and it is almost equal to the sensitivity of the E. coli pSoxS-lux biosensor, which is specific to the superoxide anion radical. The maximum response amplitude for both B. subtilis 168 pNK-MrgA and E. coli pDps was about 10 times.…”

Section: Application Of the Obtained Lux-biosensors For Assessment Of Bbh Toxicity Against Gram-positive Bacteriamentioning

confidence: 86%

“…It is known from the works [32,37] that strained hydrocarbon compounds are capable of causing DNA damage and oxidative stress, but not DNA alkylation in living cells. In E. coli cells, there are no homologues to the dinC gene of B. subtilis; therefore, the luxbiosensor E. coli pDinI, related to the SOS regulon and having a relatively high induction amplitude [28], was taken for comparison.…”

Section: Discussionmentioning

confidence: 99%

“…Mitomycin C (MitC) and methyl methanesulfonate (MMS) were obtained from Sigma-Aldrich (St. Louis, MO, USA). 2,2bis(bicyclo[2.2.1] heptane) (BBH) was synthetized as in previous work [32]. All test solutions and their dilutions were prepared immediately before use.…”

Section: Chemicalsmentioning

confidence: 99%

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Constructing of Bacillus subtilis-Based Lux-Biosensors with the Use of Stress-Inducible Promoters

Kessenikh

Novoyatlova

Bazhenov

et al. 2021

IJMS

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Here, we present a new lux-biosensor based on Bacillus subtilis for detecting of DNA-tropic and oxidative stress-causing agents. Hybrid plasmids pNK-DinC, pNK-AlkA, and pNK-MrgA have been constructed, in which the Photorhabdus luminescens reporter genes luxABCDE are transcribed from the stress-inducible promoters of B. subtilis: the SOS promoter PdinC, the methylation-specific response promoter PalkA, and the oxidative stress promoter PmrgA. The luminescence of B. subtilis-based biosensors specifically increases in response to the appearance in the environment of such common toxicants as mitomycin C, methyl methanesulfonate, and H2O2. Comparison with Escherichia coli-based lux-biosensors, where the promoters PdinI, PalkA, and Pdps were used, showed generally similar characteristics. However, for B. subtilis PdinC, a higher response amplitude was observed, and for B. subtilis PalkA, on the contrary, both the amplitude and the range of detectable toxicant concentrations were decreased. B. subtilis PdinC and B. subtilis PmrgA showed increased sensitivity to the genotoxic effects of the 2,2′-bis(bicyclo [2.2.1] heptane) compound, which is a promising propellant, compared to E. coli-based lux-biosensors. The obtained biosensors are applicable for detection of toxicants introduced into soil. Such bacillary biosensors can be used to study the differences in the mechanisms of toxicity against Gram-positive and Gram-negative bacteria.

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Section: Application Of the Obtained Lux-biosensors For Assessment Of Bbh Toxicity Against Gram-positive Bacteriasupporting

confidence: 52%

Section: Application Of the Obtained Lux-biosensors For Assessment Of Bbh Toxicity Against Gram-positive Bacteriamentioning

confidence: 86%

Section: Discussionmentioning

confidence: 99%

Section: Chemicalsmentioning

confidence: 99%

See 2 more Smart Citations

Constructing of Bacillus subtilis-Based Lux-Biosensors with the Use of Stress-Inducible Promoters

Kessenikh

Novoyatlova

Bazhenov

et al. 2021

IJMS

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“…Both gram-negative and gram-positive bacteria were used. Whole-cell bacterial lux-biosensor systems are widespread; they are used to study the general and specific toxicities of various chemical compounds and mixtures, which allows defining the mechanism of toxic action [17][18][19][20][21][22].…”

Section: Tests On Living Cellsmentioning

confidence: 99%

Anti-Inflammatory Activity of Soluble Epoxide Hydrolase Inhibitors Based on Selenoureas Bearing an Adamantane Moiety

Burmistrov

Morisseau

Babkov

et al. 2022

IJMS

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3‐Arylidene‐4‐Chromanones and 3‐Arylidene‐4‐Thiochromanones: Versatile Synthons towards the Synthesis of Complex Heterocycles

2022

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Heterocyclic compounds are well-known for their use in the synthesis of drugs and drug intermediates. This review provides a systematic survey on the chemical synthesis of complex heterocyclic compounds containing multistereocentres using 3-arylidene-4-chromanones in the last two decades. Regioselective and stereoselective synthesis of complex heterocycles involving 3-arylidene-chromanones and 3-arylidene-thiochormanones are dealt under various reaction headings such as epoxidation, Michael addition, 1,3dipolar cycloaddition, [3 + 2] and [4 + 2] cycloaddition, Diels-Alder reaction, sulfa-Michael/aldol cascade reactions, asym-metric synthesis employing bifunctional catalysts, chiral phase-transfer catalysts, chiral squaramide catalysts, etc. These synthetic highlights demonstrate the utility of 3arylidene-4-chromanones and 3-arylidene-thiochormanones as versatile synthons for rapid assemblage of complex condensed heterocyles and spiro-fused heterocycles towards the development of pharmaceutically active compounds. A brief highlight on the synthetic methods of 3-arylidene-4chromanone derivatives is also dealt. This review creates a platform for further improvement on the skeletal design of potential drug candidates.

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Genotoxic effect of 2,2’-bis(bicyclo[2.2.1] heptane) on bacterial cells

Cited by 12 publications

References 33 publications

Constructing of Bacillus subtilis-Based Lux-Biosensors with the Use of Stress-Inducible Promoters

Constructing of Bacillus subtilis-Based Lux-Biosensors with the Use of Stress-Inducible Promoters

Anti-Inflammatory Activity of Soluble Epoxide Hydrolase Inhibitors Based on Selenoureas Bearing an Adamantane Moiety

3‐Arylidene‐4‐Chromanones and 3‐Arylidene‐4‐Thiochromanones: Versatile Synthons towards the Synthesis of Complex Heterocycles

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