Lutidine derivatives for live-cell imaging of the mitochondria and endoplasmic reticulum

Singh, Deepmala; Shewale, Dipeshwari J; Sengupta, Aakash; Soppina, Virupakshi; Kanvah, Sriram

doi:10.1039/d2ob00995a

Cited by 5 publications

(5 citation statements)

References 53 publications

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“…47 This observation resonated with our earlier investigations involving styrylpyridinium derivatives. 40,48,49 Molecule TNC with a pyridinium cation showed excellent imaging of mitochondria, and this observation is consistent with its earlier reports. 50,51 Thus, a molecule with a formal positive charge ( TNC ) effectively stained mitochondria, while a zwitterionic fluorophore ( TNO ) stained lipid droplets.…”

Section: Resultssupporting

confidence: 92%

Fluorescent styryl pyridine-N-oxide probes for imaging lipid droplets

Dubey,

Mahalingavelar,

Rajput

et al. 2023

Org. Biomol. Chem.

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Section: Resultssupporting

confidence: 92%

Fluorescent styryl pyridine-N-oxide probes for imaging lipid droplets

Dubey,

Mahalingavelar,

Rajput

et al. 2023

Org. Biomol. Chem.

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“…Compounds PB4, BCM4, BCM6, GC-VII-39, GC-VII-50, GC-VI-70, and GC-VI-10 were synthesized via base-catalyzed Knoevenagel condensation between the proper pyridinium/quinolinium ions, as their iodide salts, and the corresponding aldehyde following previously reported procedures [ 11 , 12 , 38 , 39 , 40 , 41 ].…”

Section: Methodsmentioning

confidence: 99%

Evaluation of the Effects of Heteroaryl Ethylene Molecules in Combination with Antibiotics: A Preliminary Study on Control Strains

Bonomo,

Bonacci,

Bivona

et al. 2023

Antibiotics

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The discovery of compounds with antibacterial activity is crucial in the ongoing battle against antibiotic resistance. We developed two QSAR models to design six novel heteroaryl drug candidates and assessed their antibacterial properties against nine ATCC strains, including Enterococcus faecalis, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and also Salmonella enterica and Escherichia coli, many of which belong to the ESKAPE group. We combined PB4, a previously tested compound from published studies, with GC-VI-70, a newly discovered compound, with the best cytotoxicity/MIC profile. By testing sub-MIC concentrations of PB4 with five antibiotics (linezolid, gentamycin, ampicillin, erythromycin, rifampin, and imipenem), we evaluated the combination’s efficacy against the ATCC strains. To assess the compounds’ cytotoxicity, we conducted a 24 h and 48 h 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay on colorectal adenocarcinoma (CaCo-2) cells. We tested the antibiotics alone and in combination with PB4. Encouragingly, PB4 reduced the MIC values for GC-VI-70 and for the various clinically used antibiotics. However, it is essential to note that all the compounds studied in this research exhibited cytotoxic activity against cells. These findings highlight the potential of using these compounds in combination with antibiotics to enhance their effectiveness at lower concentrations while minimizing cytotoxic effects.

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“…Typically, PFP group is attached to a fluorophore of interest through an alkyl or amide linker. 17,18,[23][24][25] Here we integrated PFP to the fluorescent scaffold with dual function as electron-withdrawing moiety and as a recognition moiety for ER. 15 Based on this design approach, we synthesized three fluorophores PFC, with coumarin, PFN with naphthalene and PFB with a phenyl moiety substituted with dimethylamino electron donating group.…”

Section: Introductionmentioning

confidence: 99%