Luteolin Suppresses Ultraviolet A- and B-induced Matrix Metalloproteinase 1- and 9 Expression in Human Dermal Fibroblast Cells

Abstract

Ultraviolet (UV) irradiation induces significant changes to skin connective tissues as a result of the degradation of collagen, which is a major structural component of the extracellular matrix. This process may be mediated by matrix metalloproteinases (MMPs). In this study, we examined the protective effect of a polyphenolic flavone, luteolin, on the expression of two matrix metalloproteinases, MMP-1 and MMP-9, in UVA-and UVB-irradiated human dermal fibroblast cells. Luteolin is found in many medicinal plants as well as in a large number of vegetables, fruits and a variety of spices. It has a number of biological activities including anti-cancer, anti-oxidant, anti-inflammatory, antiallergic and immunomodulatory activities. Human dermal fibroblast (HDF) cells were treated with luteolin at 1-10 µM, then irradiated with UVA at 10 J/cm 2 and UVB at 200 mJ/cm 2 . Cells and culture supernatant were harvested 24 h after irradiation.Our results show that luteolin at 1-10 µM dose-dependently suppressed the expression of MMP-1 and MMP-9 genes in UVA and UVB-exposed HDF cells, as measured by quantitative real-time reverse transcription-polymerase chain reaction (qRT-PCR). Luteolin was also found to reduce the production of MMP-1 protein in UVA and UVBexposed HDF cells detected by enzyme-linked immunosorbent assay (ELISA) in a dose-dependent manner. The release of MMP-9 was also reduced by luteolin in UVB-irradiated HDF cells in a dose-dependent manner.Our results indicate that luteolin can inhibit UV-induced MMP-1 and MMP-9 expression in HDF cells. Therefore, they may be potentially useful in the prevention and treatment of skin photoaging.

Therapeutic Effects of Cold-Pressed Perilla Oil Mainly Consisting of Linolenic acid, Oleic Acid and Linoleic Acid on UV-Induced Photoaging in NHDF Cells and SKH-1 Hairless Mice

“…These alterations stimulate the structural and functional changes such as degradation of extracellular matrix (ECM) proteins and the upregulation of MMPs, thereby promoting skin photoaging [27]. In most previous studies, UVB irradiation onto the skin of hailress mice or keratinocytes was widely used to evaluate the therapeutic effects of natural products with anti-photogaing activity [28][29][30]. However, UVA and UVB expose induced a similar response on the MMP regulation of HDF cells although UVA radiation can be considered more suitable to study dermal fibroblast responses [28].…”

“…In most previous studies, UVB irradiation onto the skin of hailress mice or keratinocytes was widely used to evaluate the therapeutic effects of natural products with anti-photogaing activity [28][29][30]. However, UVA and UVB expose induced a similar response on the MMP regulation of HDF cells although UVA radiation can be considered more suitable to study dermal fibroblast responses [28]. Based on above evidence, our study was selected UVB radiation to examine anti-photoaging effects and mechanism of CPO on the regulation of ECM in NHDF cells.…”

Asperyellone prevents HDF cells from UVB irradiation damages: An elaborated study

“…UVB treatment significantly elevated the expression level of both MMP 2 and 9 to 78.32% and 68.3%, whereas AY pretreatment followed by UVB exposure significantly reduced the expression to 26.97% and 32.8%, which clearly authenticates the skin aging preventive property of AY. Pervin et al 43…”

“…UVB treatment significantly elevated the expression level of both MMP 2 and 9 to 78.32% and 68.3%, whereas AY pretreatment followed by UVB exposure significantly reduced the expression to 26.97% and 32.8%, which clearly authenticates the skin aging preventive property of AY. Pervin et al 43 studied the MMP 1 and 9 inhibitory profile of a flavone compound Luteolin with response to both UVA and UVB. The authors observed that pretreating the cells with 5 µM luteolin pretreatment significantly reduced the MMP elevation due to UV exposure.…”