Luteolin cooperated with metformin hydrochloride alleviates lipid metabolism disorders and optimizes intestinal flora compositions of high-fat diet mice

Ge, Xiaodong; Wang, Chang'e; Chen, Huiling; Liu, Tingting; Li-gen, Chen; Huang, Ying; Zeng, Feng; Liu, Bin

doi:10.1039/d0fo01840f

Cited by 39 publications

(32 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Mounting evidence suggests that diet represents one of the most important factors influencing the composition and structure of gut microbiota ( Cotillard et al, 2013 ), with changes in diet having the capacity to exert beneficial or harmful effects upon the composition of gut microbiota of the host. For example, it has been reported that a high-fat diet can damage the intestinal microbial environment and lead to microbiome dysregulation by reducing the amount of available carbohydrates in the colon, as well as increasing the level of intestinal oxygen stress and its own secondary metabolites ( Ge et al, 2020 ; Li et al, 2020 ). In our study, all participants were from the same region, experienced a normal/routine lifestyle and had similar nutritional patterns, including typical Chinese diets based on carbohydrates versus high-fat diets.…”

Section: Discussionmentioning

confidence: 99%

Dysbiosis of Gut Microbiota in Patients With Acute Myocardial Infarction

et al. 2021

View full text Add to dashboard Cite

Acute myocardial infarction (AMI) continues as the main cause of morbidity and mortality worldwide. Interestingly, emerging evidence highlights the role of gut microbiota in regulating the pathogenesis of coronary heart disease, but few studies have systematically assessed the alterations and influence of gut microbiota in AMI patients. As one approach to address this deficiency, in this study the composition of fecal microflora was determined from Chinese AMI patients and links between gut microflora and clinical features and functional pathways of AMI were assessed. Fecal samples from 30 AMI patients and 30 healthy controls were collected to identify the gut microbiota composition and the alterations using bacterial 16S rRNA gene sequencing. We found that gut microflora in AMI patients contained a lower abundance of the phylum Firmicutes and a slightly higher abundance of the phylum Bacteroidetes compared to the healthy controls. Chao1 (P = 0.0472) and PD-whole-tree (P = 0.0426) indices were significantly lower in the AMI versus control group. The AMI group was characterized by higher levels of the genera Megasphaera, Butyricimonas, Acidaminococcus, and Desulfovibrio, and lower levels of Tyzzerella 3, Dialister, [Eubacterium] ventriosum group, Pseudobutyrivibrio, and Lachnospiraceae ND3007 group as compared to that in the healthy controls (P < 0.05). The common metabolites of these genera are mostly short-chain fatty acids, which reveals that the gut flora is most likely to affect the occurrence and development of AMI through the short-chain fatty acid pathway. In addition, our results provide the first evidence revealing remarkable differences in fecal microflora among subgroups of AMI patients, including the STEMI vs. NSTEMI, IRA-LAD vs. IRA-Non-LAD and Multiple (≥2 coronary stenosis) vs. Single coronary stenosis groups. Several gut microflora were also correlated with clinically significant characteristics of AMI patients, including LVEDD, LVEF, serum TnI and NT-proBNP, Syntax score, counts of leukocytes, neutrophils and monocytes, and fasting serum glucose levels. Taken together, the data generated enables the prediction of several functional pathways as based on the fecal microfloral composition of AMI patients. Such information may enhance our comprehension of AMI pathogenesis.

show abstract

Section: Discussionmentioning

confidence: 99%

Dysbiosis of Gut Microbiota in Patients With Acute Myocardial Infarction

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Adams et al found that Actinomycetaceae increased significantly in patients with nonalcoholic fatty liver disease (Adams et al, 2020). Actinomycetaceae were found to be involved in liver fat synthesis and promote lipid accumulation (Ge et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

Prediction of Gut Microbial Community Structure and Function in Polycystic Ovary Syndrome With High Low-Density Lipoprotein Cholesterol

Zhu

Jiang

et al. 2021

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

Gut microbiota has been proved to be involved in the occurrence and development of many diseases, such as type 2 diabetes, obesity, coronary heart disease, etcetera. It provides a new idea for the pathogenesis of polycystic ovary syndrome (PCOS). Our study showed that the gut microbial community of PCOS with high low-density lipoprotein cholesterol (LDLC) has a noticeable imbalance. Gut microbiota of PCOS patients was significantly changed compared with CON, and these changes were closely related to LDLC. Gut microbiota may affect the metabolic level of PCOS patients through multiple metabolic pathways, and lipid metabolism disorder may further aggravate the imbalance of gut microbiota. Actinomycetaceae, Enterobacteriaceae and Streptococcaceae had high accuracy in the diagnosis of PCOS and the differentiation of subgroups, suggesting that they may play an important role in the diagnosis and treatment of PCOS in the future. Also, the model we built showed good specificity and sensitivity for distinguishing PCOS from CON (including L_CON and L_PCOS, H_CON and H_PCOS). In conclusion, this is the first report on the gut microbiota of PCOS with high LDLC, suggesting that in the drug development or treatment of PCOS patients, the difference of gut microbiota in PCOS patients with different LDLC levels should be fully considered.

show abstract

“…The protein strips were observed using the GeneGnome XRQ Chemiluminescence Imaging System (Syngene, Cambridge, UK) and ImageJ densitometry software's (National Institutes of Health, Bethesda, MD, USA) grayscale analysis for the quantitative analysis of protein. Our previous methods 5 can be used to guide the specific steps of western blotting.…”

Section: Methodsmentioning

confidence: 99%

“…The sequencing strategy was single-ended sequencing SE600 and the sequencing platform was Ion S5TMXL from Thermo Fisher. The analytical method of sequencing results was in reference to the method proposed by Ge et al 5 Table 1 List of all primers used for qPCR…”

Section: Caecal Microbiota Analysismentioning

confidence: 99%

“…3 Our previous studies found that luteolin (LUT), which was extracted and purified from Grifola frondosa using 95% ethanol, has hypolipidemic function. 5,6 In this study, we mainly studied the hypoglycemic activity in T2DM mice. The pathogenesis of hyperglycemia is chronic inflammation in the liver, leading to glucose and lipid metabolism disorders.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

6,8-(1,3-Diaminoguanidine) luteolin and its Cr complex show hypoglycemic activities and alter intestinal microbiota composition in type 2 diabetes mice

Lin

et al. 2022

Food Funct.

Self Cite

View full text Add to dashboard Cite

show abstract

Luteolin cooperated with metformin hydrochloride alleviates lipid metabolism disorders and optimizes intestinal flora compositions of high-fat diet mice

Abstract: Luteolin(LU) is a flavonoids compound and metformin hydrochloride (MH) is a kind of drug. Studies have showed that both of LU and MH have the function of hypoglycemic effect. However, there are few...

Cited by 39 publications

References 51 publications

Dysbiosis of Gut Microbiota in Patients With Acute Myocardial Infarction

Dysbiosis of Gut Microbiota in Patients With Acute Myocardial Infarction

Prediction of Gut Microbial Community Structure and Function in Polycystic Ovary Syndrome With High Low-Density Lipoprotein Cholesterol

6,8-(1,3-Diaminoguanidine) luteolin and its Cr complex show hypoglycemic activities and alter intestinal microbiota composition in type 2 diabetes mice

Contact Info

Product

Resources

About