Prediction of Gut Microbial Community Structure and Function in Polycystic Ovary Syndrome With High Low-Density Lipoprotein Cholesterol

Abstract

Gut microbiota has been proved to be involved in the occurrence and development of many diseases, such as type 2 diabetes, obesity, coronary heart disease, etcetera. It provides a new idea for the pathogenesis of polycystic ovary syndrome (PCOS). Our study showed that the gut microbial community of PCOS with high low-density lipoprotein cholesterol (LDLC) has a noticeable imbalance. Gut microbiota of PCOS patients was significantly changed compared with CON, and these changes were closely related to LDLC. Gut microbiota may affect the metabolic level of PCOS patients through multiple metabolic pathways, and lipid metabolism disorder may further aggravate the imbalance of gut microbiota. Actinomycetaceae, Enterobacteriaceae and Streptococcaceae had high accuracy in the diagnosis of PCOS and the differentiation of subgroups, suggesting that they may play an important role in the diagnosis and treatment of PCOS in the future. Also, the model we built showed good specificity and sensitivity for distinguishing PCOS from CON (including L_CON and L_PCOS, H_CON and H_PCOS). In conclusion, this is the first report on the gut microbiota of PCOS with high LDLC, suggesting that in the drug development or treatment of PCOS patients, the difference of gut microbiota in PCOS patients with different LDLC levels should be fully considered.

High Sucrose and Cholic Acid Diet Triggers PCOS-like Phenotype and Reduces Enterobacteriaceae Colonies in Female Wistar Rats

“…A high level of cholic acid in the gut could be related to the alteration of the colonic microbiota [ 16 , 17 ]. In the present study, we obtained colonic microbial samples and assessed the colonies of the Enterobacteriaceae family from both groups, growing the bacteria on MacConkey Agar.…”

“…However, some members are considered pathobionts, such as Salmonella, Shigella, and Yersinia [ 22 ]. The family has been found increased in PCOS patients [ 17 ]. Before the study, we hypothesized that Enterobacteriaceae colonies would increase under HSCA treatment.…”

“…Furthermore, cholic acid levels in the gut may have an association with the composition of the fecal microbiota in individuals with PCOS [ 16 ]. The Enterobacteriaceae family is found to be elevated in patients with PCOS and may play an important role in the pathophysiology [ 17 ]. Therefore, the objective of the present in vivo study was to evaluate the impact of the HSCA diet on the Enterobacteriaceae , insulin sensitivity, testosterone level, and histological parameters in female Rattus novergicus ovaries.…”

Polycystic Ovary Syndrome: An Evolutionary Adaptation to Lifestyle and the Environment

“…Dysbiosis is a consistent finding when looking at the microbiome of women with PCOS [140][141][142][143]. Although most studies are small, dysbiosis has consistently been found to correlate with different physiological parameters, such as obesity, sex hormones and metabolic defects [140,141,143].…”

Hormone Replacement Therapy Reverses Gut Microbiome and Serum Metabolome Alterations in Premature Ovarian Insufficiency

“…Several studies have reported alterations in the gut microbiota of patients with ovarian dysfunction, especially in patients with polycystic ovary syndrome (PCOS) ( 3 , 4 ). The gut microbiota may also affect metabolite levels in various metabolic pathways in patients with PCOS, especially in lipid metabolism, which, in turn, may further aggravate the imbalance of the intestinal microbiota ( 5 ). However, only one recent report identified altered microbial profiles in the gut microbiome of patients with POI.…”