Luteinizing Hormone-stimulated Pituitary Adenylate Cyclase-activating Polypeptide System and its Role in Progesterone Production in Human Luteinized Granulosa Cells

Park, Hyun Jeong; Choi, Bum Chae; Song, Seung-Hun; Lee, Dong Sik; Roh, Jaesook; Chun, Sang-Young

doi:10.1507/endocrj.k09e-077

Cited by 4 publications

(2 citation statements)

References 32 publications

(39 reference statements)

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“… 2004 ; Park et al. 2010 ), we improved the experimental method according to the experimental condition, and found the method which is relatively suitable for us , it reduces the damage to the cell, keeps the cell morphology intact, and avoids mixing other cells, improving the survival and purity of GCs.…”

Section: Discussionmentioning

confidence: 99%

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The effects of mouse ovarian granulosa cell function and related gene expression by suppressing BMP/Smad signaling pathway

Zhang

Wang

et al. 2018

Animal Cells and Systems

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BMP I type receptor inhibitor can selectively inhibit BMP/Smad signaling pathways, mainly by inhibiting the BMP I type receptor activity to prevent phosphorylation of Smad1, Smad5 and Smad9. The aim of the present study was to explore the effects of mouse ovarian granulosa cell function and related gene expression by suppressing BMP/Smad signaling pathway with LDN-193189(A type of BMP I type receptor inhibitor). In this study, we cultivate the original generation of mouse ovarian granular cells then collect cells and cell culture medium after treatment. Cellular localization and expression of Smad9 and P-smad9 proteins was studied by immunofluorescence (IF) in the ovarian granulosa cells of mouse; Related genes mRNA and proteins expression was checked by QRT-PCR and Western blot; Detected the concentration of related hormones by using ELISA kit; finally, the growth of the cells was analyzed by plotting cell growth curve with CCK-8 assay. The results indicate that, suppression of BMP/Smad signaling pathway can inhibit the expression of LHR and FSHR, inhibit cell proliferation and decrease E2 secretion, the mechanism of action maybe reduce the expression of smad9, at the same time, we found that the feedback regulation of smad9 may affect the expression of FSHR and cell proliferation.

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Section: Discussionmentioning

confidence: 99%

“… 1999 ; Park et al. 2010 ), then kill the mouse after 24 hours. Ovaries were removed and the GCs were extracted by puncturing ovaries.…”

Section: Methodsmentioning

confidence: 99%

The effects of mouse ovarian granulosa cell function and related gene expression by suppressing BMP/Smad signaling pathway

Zhang

Wang

et al. 2018

Animal Cells and Systems

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Expression of PACAP and PAC1 Receptor in Normal Human Thyroid Gland and in Thyroid Papillary Carcinoma

Bardosi¹,

Bardosi²,

Nagy

et al. 2016

J Mol Neurosci

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Pituitary adenylate cyclase activating polypeptide (PACAP) belongs to the vasoactive intestinal peptide-secretin-glucagon peptide family, isolated first from ovine hypothalamus. The diverse physiological effects of PACAP are known mainly from animal experiments, including several actions in endocrine glands. Alteration of PACAP expression has been shown in several tumors, but changes in expression of PACAP and its specific PAC1 receptor in human thyroid gland pathologies have not yet been investigated. Therefore, the aim of the present study was to investigate expression of PACAP and its PAC1 receptor in human thyroid papillary carcinoma, the most common endocrine malignant tumor. PACAP and PAC1 receptor expressions were investigated from thyroid gland samples of patients with papillary carcinomas. The staining intensity of follicular epithelial cells and thyroid colloid of tumor tissue was compared to that of tumor-free tissue in the same thyroid glands in a semi-quantitative way. Our results reveal that both PACAP(-like) and PAC1 receptor(-like) immunoreactivities are altered in papillary carcinoma. Stronger PACAP immunoreactivity was observed in active follicles. Colloidal PACAP immunostaining was either lacking or very weak, and more tumorous cells displayed strong apical immunoreactivity. Regarding PAC1 receptor, cells of the normal thyroid tissue showed strong granular expression, which was lacking in the tumor cells. The cytoplasm of tumor cells displayed weak, minimal staining, while in a few tumor cells we observed strong PAC1 receptor expression. This pattern was similar to that observed in the PACAP expression, but fewer in number. In summary, we showed alteration of PACAP and PAC1 receptor expression in human thyroid papillary carcinoma, indicating that PACAP regulation is disturbed in tumorous tissue of the thyroid gland. The exact role of PACAP in thyroid tumor growth should be further explored.

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Expression and functional activity of PACAP and its receptors on cumulus cells: Effects on oocyte maturation

Barberi¹,

Paolo²,

Latini³

et al. 2013

Molecular and Cellular Endocrinology

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Luteinizing Hormone-stimulated Pituitary Adenylate Cyclase-activating Polypeptide System and its Role in Progesterone Production in Human Luteinized Granulosa Cells

Cited by 4 publications

References 32 publications

The effects of mouse ovarian granulosa cell function and related gene expression by suppressing BMP/Smad signaling pathway

The effects of mouse ovarian granulosa cell function and related gene expression by suppressing BMP/Smad signaling pathway

Expression of PACAP and PAC1 Receptor in Normal Human Thyroid Gland and in Thyroid Papillary Carcinoma

Expression and functional activity of PACAP and its receptors on cumulus cells: Effects on oocyte maturation

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