The effects of mouse ovarian granulosa cell function and related gene expression by suppressing BMP/Smad signaling pathway

Zhang, Li; Wang, Hejian; Yu, Daolun; Chen, Jie; Cheng, Xing; Li, Jie; Li, Jun; Cai, Yafei

doi:10.1080/19768354.2018.1497706

Cited by 7 publications

(4 citation statements)

References 32 publications

(34 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thus, the deactivation of the TGF-beta signaling pathway is caused by the reduction of generative functions (Ross and Hill 2008 ). With BMPs and SMADs, the TGF-beta signaling pathway may be enriched for the growth and development of ovaries, such appears to be related development of various tissues at the growth stage in this result (Zhang et al 2018 ; Umair et al 2020 ). The activation of the melanogenesis pathway is related to an increase in melanin synthesis.…”

Section: Discussionmentioning

confidence: 94%

Blood transcriptome comparison between sexes and their function in 4-week Rhode Island red chickens

Kim

Choo

Cha

et al. 2022

Animal Cells and Systems

View full text Add to dashboard Cite

Sex is a major biological factor in the development and physiology of a sexual reproductive organism, and its role in the growing process is needed to be investigated in various species. We compare blood transcriptome between 5 males and 5 females in 4-week-old Rhode Island Red chickens and perform functional annotation of differentially expressed genes (DEGs). The results are as follows. 141 and 109 DEGs were located in autosomes and sex chromosomes, respectively. The gene ontology (GO) terms are significantly ( p < 0.05) enriched, which were limb development, inner ear development, positive regulation of dendrite development, the KEGG pathway the TGF-beta signaling pathway, and melanogenesis ( p < 0.05). These pathways are related to morphological maintenance and growth of the tissues. In addition, the SMAD2W and the BMP5 were involved in the TGF-beta signaling pathway, and both play an important role in maintaining tissue development. The major DEGs related to the development of neurons and synapses include the up-regulated NRN1 , GDF10 , SLC1A1 , BMP5 , NBEA, and NRXN1 . Also, 7 DEGs were validated using RT-qPCR with high correlation ( r 2 = 0.74). In conclusion, the differential expression of blood tissue in the early growing chicken was enriched in TGF-beta signaling and related to the development of neurons and synapses including SMAD2W and BMP5 . These results suggest that blood in the early growing stage is differentially affected in tissue development, nervous system, and pigmentation by sex. For future research, experimental characterization of DEGs and a holistic investigation of various tissues and growth stages will be required.

show abstract

Section: Discussionmentioning

confidence: 94%

Blood transcriptome comparison between sexes and their function in 4-week Rhode Island red chickens

Kim

Choo

Cha

et al. 2022

Animal Cells and Systems

View full text Add to dashboard Cite

show abstract

“…KRC203 and KRC360, two specific ALK2 inhibitors, suppressed the BMP9 and ALK2-induced migration and proliferation of cancer cells. Compared to LDN193189, these two inhibitors were more specific and effective in inhibiting ALK2 and were considered as promising drug candidates for the treatment of cancers or diseases with abnormal BMP9 or ALK2 signaling (93,94).…”

Section: Activin Receptor-like Kinases As a Target For Anti-tumor Thementioning

confidence: 99%

Perspectives of small molecule inhibitors of activin receptor‑like kinase in anti‑tumor treatment and stem cell differentiation (Review)

Cui

Shang

et al. 2019

Mol Med Report

View full text Add to dashboard Cite

Activin receptor-like kinases (ALKs), members of the type I activin receptor family, belong to the serine/threonine kinase receptors of the transforming growth factor-β (TGF-β) superfamily. ALKs mediate the roles of activin/TGF-β in a wide variety of physiological and pathological processes, ranging from cell differentiation and proliferation to apoptosis. For example, the activities of ALKs are associated with an advanced tumor stage in prostate cancer and the chondrogenic differentiation of mesenchymal stem cells. Therefore, potent and selective small molecule inhibitors of ALKs would not only aid in investigating the function of activin/TGF-β, but also in developing treatments for these diseases via the disruption of activin/TGF-β. In recent studies, several ALK inhibitors, including LY-2157299, SB-431542 and A-83-01, have been identified and have been confirmed to affect stem cell differentiation and tumor progression in animal models. This review discusses the therapeutic perspective of small molecule inhibitors of ALKs as drug targets in tumor and stem cells.

show abstract

“…Dysfunction or impairment of GCs can engender infertility, irregular menstrual cycles, and an array of other reproductive disorders. In cattle afflicted with cystic ovaries, the normal trajectory of folliculogenesis remains incomplete with abnormal GCs, culminating in the emergence of persistent cystic follicles [11,12].…”

Section: Introductionmentioning

confidence: 99%

Downregulation of TASK-3 Channel Induces Senescence of Granulosa Cells in the Bovine Follicular Cystic Ovary

Kim,

Woo

et al. 2024

Preprint

View full text Add to dashboard Cite

Ovarian cysts are linked to hormone imbalances and altered gene expressions, but the connection between cysts and ion channel expression is understudied. This study explored the role of TWIK-related acid-sensitive K+ (TASK) channels in bovine ovarian cyst formation. The ovarian follicles were split into small (5 to 10 mm in diameter) and large (> 25 mm in diameter) groups. Among the measured K+, Na+, and Cl- concentrations in follicular fluid (FF) obtained from small-sized follicles (SF) and large-sized follicles (LF), the K+ concentration was significantly lower in LFFF. Quantitative PCR, western blot, and immunocytochemistry data revealed that TASK-3 expression levels significantly decreased by approximately 50% in both LF and granulosa cells obtained from LF (LFGCs) compared to the corresponding controls. The TASK-3 protein was localized to the plasma membrane of GCs. The diameter of LFGCs was larger than that of SFGC. The cell swelling response to exposure to a hypotonic solution (200 mOsm/L) was highly reduced in TASK-3-overexpressing cells compared to vector-transfected cells. TASK-3-knockdown cells showed arrested growth. Senescence markers were detected in LFGCs and TASK-3-knockdown cells. These findings suggest that reduced TASK-3 expression in LF is associated with the inhibition of GC growth, leading to senescence and cyst formation.

show abstract

The effects of mouse ovarian granulosa cell function and related gene expression by suppressing BMP/Smad signaling pathway

Cited by 7 publications

References 32 publications

Blood transcriptome comparison between sexes and their function in 4-week Rhode Island red chickens

Blood transcriptome comparison between sexes and their function in 4-week Rhode Island red chickens

Perspectives of small molecule inhibitors of activin receptor‑like kinase in anti‑tumor treatment and stem cell differentiation (Review)

Downregulation of TASK-3 Channel Induces Senescence of Granulosa Cells in the Bovine Follicular Cystic Ovary

Contact Info

Product

Resources

About