Luteinizing hormone receptor splicing variants in bovine Leydig cells

Ma, Tianyi; Xiong, Qiuhong; Yuan, Bo; Jiang, Huaiyang; Gao, Yuefeng; Xu, Jin; Liu, S.Y.; Ding, Ying; Zhang, G.L.; Zhao, Yi; Zhang, J.B.

doi:10.4238/2012.june.29.4

Cited by 5 publications

(3 citation statements)

References 40 publications

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“…In addition, Leydig cells that between seminiferous tubules produce growth factors and secrete testosterone. A previous study has shown that LHR plays a key role in testosterone production and eight splicing variants of LHR gene are detected in bovine Leydig cells [105]. The androgenic stimulation of peritubular myoid cells around seminiferous tubules is also essential for normal germ cell development [106].…”

Section: The Communication Between Sertoli-sertoli Cell Andmentioning

confidence: 99%

The Function of Pre-mRNA Alternative Splicing in Mammal Spermatogenesis

Song¹,

Wang²,

Chen³

et al. 2020

Int. J. Biol. Sci.

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Alternative pre-mRNA splicing plays important roles in co-transcriptional and post-transcriptional regulation of gene expression functioned during many developmental processes, such as spermatogenesis. The studies focusing on alternative splicing on spermatogenesis supported the notion that the development of testis is regulated by a higher level of alternative splicing than other tissues. Here, we aim to review the mechanisms underlying alternative splicing, particularly the splicing variants functioned in the process of spermatogenesis and the male infertility. There are five points regarding the alternative splicing including ⅰ) a brief introduction of alternative pre-mRNA splicing; ⅱ) the alternative splicing events in spermatogenesis-associated genes enriched in different stages of spermatogenesis; ⅲ) the mechanisms of alternative splicing regulation, such as splicing factors and m 6 A demethylation; ⅳ) the splice site recognition and alternative splicing, including the production and degradation of abnormal transcripts caused by gene variations and nonsense-mediated mRNA decay, respectively; ⅴ) abnormal alternative splicing correlated with male infertility. Taking together, this review highlights the impacts of alternative splicing and splicing variants in mammal spermatogenesis and provides new insights of the potential application of the alternative splicing into the therapy of male infertility.

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Section: The Communication Between Sertoli-sertoli Cell Andmentioning

confidence: 99%

The Function of Pre-mRNA Alternative Splicing in Mammal Spermatogenesis

Song¹,

Wang²,

Chen³

et al. 2020

Int. J. Biol. Sci.

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“…(Genbank 2020). Este gen se expresa en las gónadas junto con el receptor de la FSH (FSHR), mientras que receptor el de la GnRH lo hace en la hipófisis anterior, está sujeto a corte y empalme alternativo (splicing alternativo), dando origen a diferentes variantes del mRNA del gen (Ma et al, 2012); en bovinos se han reportado variantes que carecen del exón 10 , deleción parcial del exón 11 (Robert et al, 2003;Nogueira et al, 2007) y del exón 3 (Nogueira et al, 2007).…”

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Polimorfismos del gen del receptor para la hormona luteinizante (LHR – LHGCR) en folículos ováricos de vacas Romosinuano y Brahman ciclando

2020

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El gen del receptor para la hormona luteinizante (LHR-LHCGR) es un gen altamente conservado y su mRNA origina diferentes variantes por corte y empalme alternativo (splicing alternativo). En bovinos se han reportado variantes que traducen en proteína pero ésta no atraviesa la membrana celular; en humanos ante una deleción del exón 10 no hay respuesta a la acción de la LH, por lo tanto, es razonable asumir que las diferencias en la respuesta a la LH estén relacionadas con la expresión de los LHR. El objetivo de este trabajo fue comparar entre la raza criolla Colombiana Romosinuano y la raza Brahman los posibles polimorfismos del ARNm del gen como potenciales indicadores de fertilidad. Se tomaron 5 vacas ciclando de cada raza se extirpó el ovario que contenía un folículo preovulatorio, se retiró la pared folicular, se extrajo el RNA total y se sintetizó, amplificó, secuenció y comparó el ADNc contra secuencias ya reportadas, presentando una alta similitud. Se concluye que en estas razas la ausencia de variantes en la expresión del gen, estaría asociada a buen desempeño reproductivo.

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“…The genetic diversity in crossbred beef cows demonstrates that ILSTS002 and BMS3004 are associated with the luteinizing hormone-β (LHβ) gene on BTA18 that is associated with reproductive performance (SILVEIRA et al, 2013). In addition, receptors for luteinizing hormone (LHR) and follicle stimulating hormone (FSHR) genes located at BTA11 are also involved in the regulation of several essential reproductive processes occurring at the gonadal level (CHENG, 1975;MA et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Candidate genes related to reproductive traits of Hereford and Braford bulls

Menegassi

Pereira

Aguiar

et al. 2018

SCA

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This study emphasized the importance of using candidate genes in predicting semen quality in bulls that can be used in cow-calf production. The aim of this study was to evaluate some candidate genes related to reproductive traits in Braford and Hereford bulls. All bulls (n=188) were submitted to breeding soundness evaluations at 24, 28, 32, and 36 months of age. The microsatellite markers ILSTS002 and BMS3004 associated with the luteinizing hormone-β (LHβ) gene, IDVGA-51 to leptin (LEP) gene, HEL5 and AFZ1 within the IGF-IR gene, and two SNP markers (LHR and FSHR) associated with the LHR and FSHR genes, respectively, were evaluated by the amplification of DNA products. The variation in the IDVGA-51 allele 177-185 showed polymorphic information content (PIC) associated with sperm motility and vigor traits in Hereford bulls. Hereford animals showed PIC of 0.36 to 0.75% along with expected heterozygosity (H) of 0.49 to 0.78%. Braford bulls that indicated the ILSTS002 allele 137-175 and AFZ1 allele 113-119 showed PIC associated with major and minor defects, respectively. The PIC ranged from 0.28 to 0.78%, with an expected H of 0.35 to 0.81%. AFZ1 allele 121-127 had the highest minor defects and ILSTS002 allele 125-135 showed the highest major defects from ejaculated semen in Braford bulls. In addition, IDVGA-51 allele 175 showed lower motility and vigor in Hereford bulls. The markers AFZ1 (IGF-IR) and ILSTS002 (LHβ) in Braford and IDVGA-51 (LEP) in Hereford bulls were effective in verifying the reproductive traits of the bulls. Key words: Molecular markers. Single nucleotide polymorphisms. Microsatellites. Genetic variability. Braford and Hereford breeds. ResumoEste estudo enfatizou a importância do uso de genes candidatos na predição da qualidade do sêmen em touros que podem ser utilizados na produção de bezerros. O objetivo deste estudo foi avaliar alguns

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Luteinizing hormone receptor splicing variants in bovine Leydig cells

Cited by 5 publications

References 40 publications

The Function of Pre-mRNA Alternative Splicing in Mammal Spermatogenesis

The Function of Pre-mRNA Alternative Splicing in Mammal Spermatogenesis

Polimorfismos del gen del receptor para la hormona luteinizante (LHR – LHGCR) en folículos ováricos de vacas Romosinuano y Brahman ciclando

Candidate genes related to reproductive traits of Hereford and Braford bulls

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