Luteimonas chenhongjianii, a novel species isolated from rectal contents of Tibetan Plateau pika (Ochotona curzoniae)

Abstract

<jats:p>Two Gram-stain-negative, strictly aerobic, bright-yellow-pigmented and rod-shaped bacteria (strains 100069 and 100111<jats:sup>T</jats:sup>) with a single polar flagellum were isolated from the rectal contents of plateau pika (<jats:italic>Ochotona curzoniae</jats:italic>). Based on the results of nearly full-length 16S rRNA gene sequence and phylogenetic analyses, strains 100069 and 100111<jats:sup>T</jats:sup> belong to the genus <jats:italic> <jats:named-content content-type="genus"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.2238" xlink:type="simple">Luteimonas</jats:ext-link> </jats:named-content> </jats:italic>, and are closest to <jats:italic> <jats:named-content content-type="species"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.31562" xlink:type="simple">Luteimonas rhizosphaerae</jats:ext-link> </jats:named-content> </jats:italic> 4-12<jats:sup>T</jats:sup> (98.02 % similarity), <jats:italic> <jats:named-content content-type="species"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.14231" xlink:type="simple">Luteimonas aestuarii</jats:ext-link> </jats:named-content> </jats:italic> B9<jats:sup>T</jats:sup> (97.8 %) and <jats:italic> <jats:named-content content-type="species"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.28313" xlink:type="simple">Luteimonas terrae</jats:ext-link> </jats:named-content> </jats:italic> THG-MD21<jats:sup>T</jats:sup> (97.74 %). The DNA G+C contents of these two isolates were 68.30 mol% and 68.29 mol%, respectively. The highest average nucleotide identity (ANI) value between strain 100111<jats:sup>T</jats:sup> and its closely related species was 83.34 %, well below the threshold of 95–96 %. The major cellular fatty acids were iso-C<jats:sub>11 : 0</jats:sub>, iso-C<jats:sub>15 : 0</jats:sub> and iso-C<jats:sub>17 : 1</jats:sub> <jats:italic> </jats:italic>ω9. Polar lipid content was dominated by diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, an unidentified phospholipid and an unidentified lipid. Ubiquinone-8 (Q-8) was the predominant respiratory quinone. These two isolates grew optimally at 35–37 °C, pH 7.0–8.0 and with 1.0 % (w/v) NaCl. The results of ANI analysis and other characteristics obtained from our polyphasic study showed that strains 100069 and 100111<jats:sup>T</jats:sup> represent a novel species in genus <jats:italic> <jats:named-content content-type="genus"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.2238" xlink:type="simple">Luteimonas</jats:ext-link> </jats:named-content> </jats:italic>, for which the name <jats:italic>Luteimonas chenhongjianii</jats:italic> sp. nov. (type strain 100111<jats:sup>T</jats:sup>=DSM 104077<jats:sup>T</jats:sup>=CGMCC 1.16429<jats:sup>T</jats:sup>) is proposed.</jats:p>

Gordonia jinghuaiqii sp. nov. and Gordonia zhaorongruii sp. nov., isolated from Tibetan Plateau wildlife

“…The morphological traits, chemotaxonomic and phenotypic characteristics were investigated using cells cultured under their optimal conditions (detailed information in the species descriptions), and these four strains and some of their respective reference strains were cultured under the same conditions in parallel, for which all operations were performed as described in a previous study [15]. The cellular fatty acid (CFA) profiles, polar lipids, respiratory quinones, whole-cell sugar and cell-wall peptidoglycan were detected as in previous studies [15,36].…”

“…While studying the microflora diversity of Tibetan Plateau wildlife (PR China; e.g. wild ass, antelope, gazelle, vultures, plateau pika, marmot and snow finch), quite a few novel species have been uncovered in our laboratory, including Nocardioides yefusunii , Streptococcus pantholopis , Microbacterium wangchenii , Actinomyces liubingyangii , Luteimonas chenhongjianii , Lactobacillus xujianguonis and Vagococcus xieshaowenii [11–17]. The 16S rRNA gene sequences (described below) of these four hitherto unknown micro-organisms (zg-686 T , zg-691, HY186 T and HY189) isolated from marmot ( Marmota himalayana ) and wild ass ( Equus kiang ) have low similarity to those of known species in the GenBank database.…”

Luteimonas deserti sp. nov., a novel strain isolated from desert soil

“…Menaquinones were extracted from 100 mg freeze-dried cells with chloroform-methanol (2 : 1, v/v) solution and analysed as described by Komagata and Suzuki [44] using high-performance liquid chromatography (CTO-20A, Shimadzu). The only respiratory quinone of strain SJ-16 T was ubiquinone Q-8, similar to L. chenhongjianii 100111 T [7], L. terrae JCM 30122 T [45] and other strains in the genus Luteimonas [2].…”

“…The only respiratory quinone of strain SJ-16 T was ubiquinone Q-8, similar to L. chenhongjianii 100111 T [7], L. terrae JCM 30122 T [45] and other strains in the genus Luteimonas [2].…”

“…Several recently identified species include 'Luteimonas weifangensis' [4], 'Luteimonas granuli' [5], 'Luteimonas cellulosilyticus' [6], Luteimonas gilva [2], Luteimonas chenhongjianii [7], Luteimonas yindakuii [8] and Luteimonas lumbrici [9]. Luteimonas strains can be found in various habitats, such as bensulfuron-methyl contaminated watermelon and farmland soil, fresh water or wastewater treatment plants, sediment, food waste, wormcasts, rectal contents, and plant leaves or rhizosphere [2,[4][5][6][7][8][9][10][11][12][13][14][15]. A few Luteimonas strains have been found that can degrade cellulose [6], synthesize siroheme [2], enhance oil recovery [16], and enhance the rate of degradation of petroleum hydrocarbons in diesel-contaminated soil [17].…”