Lung-on-a-chip platforms for modeling disease pathogenesis

Dellaquila, Alessandra; Thomée, Emma; McMillan, Alexander H.; Lesher‐Pérez, Sasha Cai

doi:10.1016/b978-0-12-817202-5.00004-8

Cited by 8 publications

(16 citation statements)

References 187 publications

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“…Alveoli are air filled sacs that exchange gas with a highly branched microvasculature at the distal ends of the lung [70],[71] . A thin membrane lined with pneumocytes regulates O 2 and CO 2 exchange between red blood cells and the gas phase (Figure 7A) [2],[72] . Alveoli diameters range from approx.…”

Section: Resultsmentioning

confidence: 99%

“…Organ-on-a-chip systems that mimic relevant aspects of human tissue physiology have emerged as powerful tools to study complex inter and intra tissue phenomena [1], [2] . Such microfluidic systems combine biological structures with perfusion to control and manipulate cellular microenvironments and to ensure sufficient nutrient supply for short and long term culturing [3], [4] .…”

Section: Introductionmentioning

confidence: 99%

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Engineering Principles and Algorithmic Design Synthesis for Ultracompact Bio-Hybrid Perfusion Chip

Erben

Kellerer

Lissner³

et al. 2022

Preprint

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Bioinspired 3D microfluidic systems that combine vascularization with extracellular matrix architectures of organotypic geometry, composition and biophysical traits can help advance our understanding of microorgan physiology. Here, two-photon stereolithography is adopted to fabricate freestanding perfusable 3D cell scaffolds with micrometer resolution from gelatin methacryloyl hydrogel derived from extracellular matrix protein. As a proof of concept, we introduce an ultracompact bio-hybrid chip layout to demonstrate perfusion and cell seeding of double-digit μm proteinaceous channels. This perfusion chip consists of a standardized microfluidic interface fabricated from standard resin and a GM10 bioink channel printed atop of this interface. In addition, we demonstrate that algorithmic design synthesis can recapitulate intact alveoli and capillary networks with tunable design parameters to implement vascularized alveolar tissue models. This approach will allow for a systematic investigation of cell-cell and tissue dynamics in response to defined structural, mechanical and bio-molecular cues and is ultimately scalable to fabricate organ-on-a-chip systems.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Engineering Principles and Algorithmic Design Synthesis for Ultracompact Bio-Hybrid Perfusion Chip

Erben

Kellerer

Lissner³

et al. 2022

Preprint

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show abstract

“…[ 50 ] The recent combination with tissue engineering approaches and biomaterials has accelerated the transition from traditional nonbiomimetic materials (glass, silicon, and polydimethylsiloxane (PDMS)) and 2D cell culture to 3D ECM‐like hydrogel‐based platforms. [ 17 , 51 ] Microfluidic‐based vascular models have been used to study the response of endothelium to a plethora of stimuli under both physiological and pathological conditions, [ 6 , 52 , 53 ] the interaction between endothelium and parenchyma in organ‐specific vascular platforms and to understand key factors in vasculogenesis and angiogenesis processes. [ 43 , 54 ] Microfluidics has been used as well for investigating the interaction between blood cells (platelets, leukocytes, and red blood cells) and vasculature and their response to mechanical or biochemical cues, which cannot be studied with static traditional in vitro platforms.…”

Section: Vascularization Approaches For Physiologically Relevant 3d Modelsmentioning

confidence: 99%

“…Exposure to cytokines and nanoparticles showed the active role of vasculature and mechanical forces under inflammatory conditions, underlying the need to integrate these components to build complex in vitro platforms capable of recreating physiological organ functions. [ 53 ]…”

Section: Vascularization Approaches For Physiologically Relevant 3d Modelsmentioning

confidence: 99%

In Vitro Strategies to Vascularize 3D Physiologically Relevant Models

et al. 2021

Self Cite

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Vascularization of 3D models represents a major challenge of tissue engineering and a key prerequisite for their clinical and industrial application. The use of prevascularized models built from dedicated materials could solve some of the actual limitations, such as suboptimal integration of the bioconstructs within the host tissue, and would provide more in vivo-like perfusable tissue and organ-specific platforms. In the last decade, the fabrication of vascularized physiologically relevant 3D constructs has been attempted by numerous tissue engineering strategies, which are classified here in microfluidic technology, 3D coculture models, namely, spheroids and organoids, and biofabrication. In this review, the recent advancements in prevascularization techniques and the increasing use of natural and synthetic materials to build physiological organ-specific models are discussed. Current drawbacks of each technology, future perspectives, and translation of vascularized tissue constructs toward clinics, pharmaceutical field, and industry are also presented. By combining complementary strategies, these models are envisioned to be successfully used for regenerative medicine and drug development in a near future.

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“…The key function of the respiratory system is to supply oxygen, remove carbon dioxide, maintain blood pH and filter xenobiotics. The precise structure of tissues and a number of complex biochemical and biophysical factors allow gas–liquid exchange, since the membrane is only a few microns thick [ 37 ]. Conventional culture methods do not provide cells with mechanical stimuli, such as shear stress, stress or compression, or physical stimuli such as substrate rigidity and specific dimensional and geometric nanostructures.…”

Section: Organs On-a-chipmentioning

confidence: 99%

Novel Strategies in Artificial Organ Development: What Is the Future of Medicine?

Klak¹,

Bryniarski²,

Kowalska³

et al. 2020

Micromachines

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The technology of tissue engineering is a rapidly evolving interdisciplinary field of science that elevates cell-based research from 2D cultures through organoids to whole bionic organs. 3D bioprinting and organ-on-a-chip approaches through generation of three-dimensional cultures at different scales, applied separately or combined, are widely used in basic studies, drug screening and regenerative medicine. They enable analyses of tissue-like conditions that yield much more reliable results than monolayer cell cultures. Annually, millions of animals worldwide are used for preclinical research. Therefore, the rapid assessment of drug efficacy and toxicity in the early stages of preclinical testing can significantly reduce the number of animals, bringing great ethical and financial benefits. In this review, we describe 3D bioprinting techniques and first examples of printed bionic organs. We also present the possibilities of microfluidic systems, based on the latest reports. We demonstrate the pros and cons of both technologies and indicate their use in the future of medicine.

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Lung-on-a-chip platforms for modeling disease pathogenesis

Cited by 8 publications

References 187 publications

Engineering Principles and Algorithmic Design Synthesis for Ultracompact Bio-Hybrid Perfusion Chip

Engineering Principles and Algorithmic Design Synthesis for Ultracompact Bio-Hybrid Perfusion Chip

In Vitro Strategies to Vascularize 3D Physiologically Relevant Models

Novel Strategies in Artificial Organ Development: What Is the Future of Medicine?

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