Lung dendritic cells migrate to the spleen to prime long-lived TCF1 ^hi memory CD8 ⁺ T cell precursors after influenza infection

Abstract

Lung-derived dendritic cells carry influenza antigens to the spleen after egressing the lymph node by an S1P/S1PR-dependent mechanism.

Granulocyte Macrophage-Colony Stimulating Factor Produces a Splenic Subset of Monocyte-Derived Dendritic Cells That Efficiently Polarize T Helper Type 2 Cells in Response to Blood-Borne Antigen

“…In CCR2 knockout (KO) mice treated with GM-CSF (Figure 7A), classical monocytes as well as pre-GMiDCs and GMiDCs were significantly reduced in the spleen, indicating that splenic GMiDCs substantially derived from BM classical monocytes. Meanwhile, the deficiency of CCR7, an essential chemokine receptor for DC migration from tissues to lymphoid organs (57) including the spleen (58), did not alter the fraction of G M i D C s i n t h e s p l e e n o f G M -C S F -t r e a t e d m i c e (Supplementary Figure 30), suggesting that the development of GMiDCs was not dependent on CCR7. Given that the transcription factor Irf4 is upregulated in both cDC2s and GMiDCs under GM-CSF condition (Supplementary Figure 20), we analyzed the Irf4 fl/fl mice crossed with the Itgaxcre mice that express Cre recombinase in DCs (59).…”

Mucosal immune responses to infection and vaccination in the respiratory tract

“…Recently, Jenkins et al. described a pathway for murine mDCs, which traffic from the lungs of influenza-infected mice to draining LNs and can re-enter circulation and localize in the spleen ( Jenkins et al., 2021 ). Here, these mDCs activate CD8 T cell responses, which, intriguingly, were found to be longer lived than traditional LN-primed CD8 T cells.…”

TNF-α sculpts a maturation process in vivo by pruning tolerogenic dendritic cells

“…Therefore, CCR7 hi cDCs are considered to have migratory properties, and these cDCs play multiple roles in the initiation of effector responses under pro-inflammatory conditions as well as in the orchestration of tolerance, particularly that limiting anti-tumor responses ( Bajana et al, 2012 ; GeurtsvanKessel et al, 2008 ; Ghiringhelli et al, 2005 ; Harimoto et al, 2013 ; Jenkins et al, 2021 ; Kim et al, 2010 ; Krishnaswamy et al, 2018 ; Maier et al, 2020 ; Scheinecker et al, 2002 ; Wang et al, 2019 ). Migratory cDCs transport antigens from parenchymal tissues to the lymph nodes in the steady state ( Randolph et al, 2008 ) and, in the case of some respiratory infections, also to the spleen ( Jenkins et al, 2021 ; Sichien et al, 2017 ). However, systemic soluble antigens are also delivered directly to cDCs present in the lymphoid organs, such as to spleen via the bloodstream ( Bourque and Hawiger, 2022 ; Eisenbarth, 2019 ).…”