Luffa acutangula and Lippia nodiflora leaf extract induces growth inhibitory effect through induction of apoptosis on human lung cancer cell line

Vanajothi, Ramar; Sudha, A.; Manikandan, R.; Rameshthangam, Palanivel; Srinivasan, Pappu

doi:10.1016/j.bionut.2012.03.002

Cited by 28 publications

(15 citation statements)

References 29 publications

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“…Despite the recent advance in therapeutic methods, human cancer remains a leading cause of mortality worldwide (1,2). Moreover, the incidence of many types of cancers, including melanoma, prostate, breast, liver and lung cancer, continues to increase (3,4). Human lung cancer continues to be the leading cause of cancer-related mortality among males in developing countries (5).…”

Section: Introductionmentioning

confidence: 99%

The cathelicidin-BF Lys16 mutant Cbf-K16 selectively inhibits non-small cell lung cancer proliferation in vitro

Tian

Wang

et al. 2013

Oncology Reports

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The 30-amino acid antimicrobial peptide Cbf-K16 is a cathelicidin-BF (BF-30) Lys16 mutant derived from the snake venom of Bungarus fasciatus. Our previous study found that BF-30 selectively inhibited the proliferation of the metastatic melanoma cell line B16F10 in vitro and in vivo, but had a negligible effect on human lung cells. In the present study, it was demonstrated for the first time that Cbf-K16 selectively inhibits the proliferation of lung carcinoma cells in vitro, with low toxicity to normal cells. The half-maximal inhibitory concentrations (IC50) of Cbf-K16 against H460 human non-small cell lung carcinoma cells and mouse Lewis lung cancer cells were only 16.5 and 10.5 µM, respectively, which were much less compared to that of BF-30 (45 and 40.3 µM). Data using a transmission electron microscope (TEM) assay showed that, at 20 and 40 µM, Cbf-K16 induced the rupture of the cytoplasmic membrane, which was consistent with data obtained from lactate dehydrogenase (LDH) release assays. The LDH release increased from 17.8 to 52.9% as the duration and dosage of Cbf-K16 increased. Annexin V-ﬂuorescein and propidium iodide staining assays indicated that there were no obvious apoptotic effects at the different dosages and times tested. In H460 cells, the rate of genomic DNA binding increased from 51.9 to 86.8% as the concentration of Cbf-K16 increased from 5 to 10 µM. These data indicate that Cbf-K16 selectively inhibits the proliferation of lung carcinoma cells via cytoplasmic membrane permeabilization and DNA binding, rather than apoptosis. Although Cbf-K16 displayed significant cytotoxic activity (40 µM) against tumor cells, in splenocytes no significant inhibitory effect was observed and hemolysis was only 5.6%. These results suggest that Cbf-K16 is a low-toxicity anti-lung cancer drug candidate.

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Section: Introductionmentioning

confidence: 99%

The cathelicidin-BF Lys16 mutant Cbf-K16 selectively inhibits non-small cell lung cancer proliferation in vitro

Tian

Wang

et al. 2013

Oncology Reports

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“…The anticancer activities of Luffa sp. aerial parts against various types of cancer have been previously reported (e.g., human neuronal glioblastoma cells (U343) and human lung cancer cells (A549) [59], human lung cancer cell line (NCI-H460) [60], Ehrlich ascites carcinoma (EAC) [61], Dalton's lymphoma ascites (DLA) [62]); however, to the best of our knowledge there are no reports regarding the cytotoxic effects of luffa seed oil against cancer cell lines and the results of our study could be useful for the exploitation of this underutilized species. Linseed is also well known for its anticancer activities, especially against hormone related cancers such as breast, prostate, and colon cancer, due to its high content in α-linolenic acid and polyphenols which reduce human estrogen receptorpositive tumors and inhibit cancer cells proliferation [63][64][65].…”

Section: Resultsmentioning

confidence: 99%

Antimicrobial Properties, Cytotoxic Effects, and Fatty Acids Composition of Vegetable Oils from Purslane, Linseed, Luffa, and Pumpkin Seeds

et al. 2021

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In the present study, the antimicrobial and cytotoxic activities, as well as the fatty acids composition in vegetable seed oils from linseed, purslane, luffa, and pumpkin were evaluated. For this purpose, two linseed oils and one luffa oil were commercially obtained, while purslane and pumpkin oils were obtained from own cultivated seeds. The results showed a variable fatty acids composition among the tested oils, with α-linolenic, linoleic, oleic, palmitic, and stearic acid being the most abundant compounds. In regards to particular oils, linseed oils were a rich source of α-linolenic acid, luffa and pumpkin oil were abundant in linoleic acid, while purslane oil presented a balanced composition with an almost similar amount of both fatty acids. Luffa oil was the most effective against two of the tested cancer cell lines, namely HeLa (cervical carcinoma) and NCI-H460 (non-small cell lung cancer), while it also showed moderate toxicity against non-tumor cells (PLP2 cell line). Regarding the antibacterial activity, linseed oil 3 and pumpkin oil showed the highest activity against most of the tested bacteria (especially against Enterobacter cloacae and Escherichia coli) with MIC and MBC values similar to the used positive controls (E211 and E224). All the tested oils showed significant antifungal activities, especially luffa and pumpkin oil, and for most of the tested fungi they were more effective than the positive controls, as for example in the case of Aspergillus versicolor, A. niger, and Penicillium verrucosum var. cyclopium. In conclusion, the results of our study showed promising antimicrobial and cytotoxic properties for the studied seed oils which could be partly attributed to their fatty acids composition, especially the long-chain ones with 12–18 carbons.

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“…Anticancer Leaf extract Exhibited induced apoptosis in human lung cancer (NCI-H460) cells showed high antiproliferative activity. (Vanajothi et al, 2012) Methanolic extract…”

Section: Essential Oilmentioning

confidence: 99%

Botany, Traditional Uses and Pharmacology of Bukkun Booti - Phyla nodiflora (L.) Greene: An Underexposed Botanical Drug of AYUSH-Unani System

Husain

Pratap

Alam

et al. 2021

ijrps

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Bukkun Booti (Phyla nodiflora (L.) Greene; Syn. Lippia nodiflora (L.) Michx.), of Verbenaceae, is a fast-growing creeping perennial medicinal herb, has a very long history for human use as it is generally distributed throughout the world. The plant has been traditionally used and recorded in AYUSH-Ayurveda, Unani and Siddha systems of medicine. In the Unani System of Medicine (USM), the Bukkun Booti is used for detoxification of the blood. It is useful in Bawāsīr (bleeding piles), Ru‘af (epistaxis), Hasāh al-Mathāna (cystolithiasis), Sozāk (gonorrhea) ‘Usr al-Bawl (dysuria) and Hummayat (fevers). Ethno-medicinally, it has been widely used as a traditional folk medicine to treat and cure ailments by the local tribal and other communities. The entire plant is diuretic, febrifuge, stomachic and astringent; good for ulcers, wounds, asthma, bronchitis, knee-joints and to ladies after delivery. Bukkun Booti is a rich source of antioxidants and secondary metabolites, and aerial parts are reported to contain phenolic compounds (flavonoids), which are found to have a broad array of reported pharmacological actions such as; antibacterial, anti-inflammatory, antioxidant, antidiabetic and hepatoprotective properties. The purpose of this review paper is to bring together the available information on the current status and therapeutic uses of Bukkun Booti in the Unani System of Medicine and to discuss the botany and importance of the plant on the basis of folk uses, pharmacological activities and chemical constituents. The potential characteristics of P. nodiflora could be utilized more efficiently by linking the bridge of traditional uses, pharmacology and phytochemistry through an integrated approach.

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Luffa acutangula and Lippia nodiflora leaf extract induces growth inhibitory effect through induction of apoptosis on human lung cancer cell line

Cited by 28 publications

References 29 publications

The cathelicidin-BF Lys16 mutant Cbf-K16 selectively inhibits non-small cell lung cancer proliferation in vitro

The cathelicidin-BF Lys16 mutant Cbf-K16 selectively inhibits non-small cell lung cancer proliferation in vitro

Antimicrobial Properties, Cytotoxic Effects, and Fatty Acids Composition of Vegetable Oils from Purslane, Linseed, Luffa, and Pumpkin Seeds

Botany, Traditional Uses and Pharmacology of Bukkun Booti - Phyla nodiflora (L.) Greene: An Underexposed Botanical Drug of AYUSH-Unani System

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