&lt;title&gt;Multispectral imaging with a liquid crystal tunable filter&lt;/title&gt;

Miller, Peter J.; Hoyt, Clifford

doi:10.1117/12.198889

Cited by 40 publications

(27 citation statements)

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“…The Maestro™ system (CRI, Woburn, MA) of multispectral approaches to small animal imaging ( Figure 1) is based on the use of liquid crystal tunable filters (LCTFs 1 ; for descriptions of LCTF core technology, see Miller and Hoyt 1995, Gat 2000, and Hoyt et al 2001; Berman and Moore 1994 compare the LCTF method in some detail to other spectral imaging techniques).…”

Section: Lctf-based Spectral Imaging Hardwarementioning

confidence: 99%

Multiplexing with Multispectral Imaging: From Mice to Microscopy

Levenson

Lynch²,

Kobayashi

et al. 2008

ILAR Journal

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Increasing sophistication in the design and application of biological models as well as the advent of novel fluorescent probes have led to new demands on molecular imaging systems to deliver enhanced sensitivity, reliable quantitation, and the ability to resolve multiple simultaneous signals. Sensitivity is limited, especially in the visible spectral range, by the presence of ubiquitous autofluorescence signals (mostly arising from the skin and gut), which need to be separated from those of targeted fluorophores. Fluorescence-based imaging is also affected by absorbing and scattering properties of tissue in both the visible and to a lesser extent the near-infrared (NIR) regions. However, the small size of typical animal models (usually mice) often permits the detection of enough light arising even from relatively deep locations to allow the capture of signals with an acceptable signal-to-noise ratio. Multispectral imaging, through its ability to separate autofluorescence from label fluorescence, can increase sensitivity as much as 300 times compared to conventional approaches, and concomitantly improve quantitative accuracy. In the NIR region, autofluorescence, while still significant, poses less of a problem. However, the task of disentangling signals from multiple fluorophores remains. Multispectral imaging allows the separation of five or more fluorophores, with each signal quantitated and visualized separately. Preclinical small animal imaging is often accompanied by microscopic analysis, both before and after the in vivo phase. This can involve tissue culture manipulations and/or histological examination of fixed or frozen tissue. Due to the same advantages in sensitivity, quantitation, and multiplexing, microscopy-based multispectral techniques form an excellent complement to in vivo imaging.

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Section: Lctf-based Spectral Imaging Hardwarementioning

confidence: 99%

Multiplexing with Multispectral Imaging: From Mice to Microscopy

Levenson

Lynch²,

Kobayashi

et al. 2008

ILAR Journal

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“…Birefringence filters include mainly birefringent plates and liquid crystals (LC) cells which can be controlled and tuned. Tunable filters are most commonly used for optical telecommunication 4 but lately also for biomedical spectral imaging such as Raman chemical imaging 5,6 and fluorescence microscopy applications. The design of an optical filter is against two major parameters, the bandwidth defined the full width at half maximum (FWHM) of its main output peak and the free spectral range (FSR) or the dynamic range, these two parameters are very desirable for wavelength filtering and tuning.…”

Section: Introductionmentioning

confidence: 99%

Liquid crystal tunable filters and polarization controllers for biomedical optical imaging

et al. 2008

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Liquid crystal (LC) devices exhibit fast and strong tuning and switching capabilities using small voltages and can be miniaturized thus have a great potential to be used with miniature optical imaging systems for biomedical applications. LC devices designed specifically for integration into biomedical optical imaging systems are presented. Using a combination of one or two LC retarders we obtained polarimetric imaging of the skin. LC tunable filters with high dynamic range and large throughput are designed for hyperspectral imaging and for spectral domain optical coherence tomography. The designs are based on several concepts both using the classical stack of retarders and using more modern designs based on single layer in a waveguide or in a Fabry-Perot cavity.

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“…There are different types of multispectral imaging, among them most of them are filter-based which are also the one of our interest in this paper. In a typical filter-based imaging systems, either a set of traditional optical filters in a filter wheel, or a tunable filter 12,13 capable of many different configurations are employed. It acquires a multispectral image by taking multiple shots, one at a time with one filter.…”

Section: Introductionmentioning

confidence: 99%

One-shot multispectral color imaging with a stereo camera

Shrestha

Hardeberg

Mansouri

2011

Digital Photography VII

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Multispectral color imaging is a promising technology, which can solve many of the problems of traditional RGB color imaging. However, it still lacks widespread and general use because of its limitations. State of the art multispectral imaging systems need multiple shots making it not only slower but also incapable of capturing scenes in motion. Moreover, the systems are mostly costly and complex to operate. The purpose of the work described in this paper is to propose a one-shot six-channel multispectral color image acquisition system using a stereo camera or a pair of cameras in a stereoscopic configuration, and a pair of optical filters. The best pair of filters is selected from among readily available filters such that they modify the sensitivities of the two cameras in such a way that they get spread reasonably well throughout the visible spectrum and gives optimal reconstruction of spectral reflectance and/or color. As the cameras are in a stereoscopic configuration, the system is capable of acquiring 3D images as well, and stereo matching algorithms provide a solution to the image alignment problem. Thus the system can be used as a "two-in-one" multispectral-stereo system. However, this paper mainly focuses on the multispectral part. Both simulations and experiments have shown that the proposed system performs well spectrally and colorimetrically.

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<title>Multispectral imaging with a liquid crystal tunable filter</title>

Cited by 40 publications

References 0 publications

Multiplexing with Multispectral Imaging: From Mice to Microscopy

Multiplexing with Multispectral Imaging: From Mice to Microscopy

Liquid crystal tunable filters and polarization controllers for biomedical optical imaging

One-shot multispectral color imaging with a stereo camera

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