&lt;title&gt;Laterally modulated excitation microscopy: improvement of resolution by using a diffraction grating&lt;/title&gt;

Heintzmann, Rainer; Cremer, Christoph

doi:10.1117/12.336833

Cited by 538 publications

(405 citation statements)

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“…Because of diffraction, conventional microscopes cannot resolve structure details that are closer than half the wavelength of the detected light (19), limiting the lateral resolution to ;200 nm. This barrier seemed to be insuperable for more than a century, but today's superresolution light-optical technologies can bypass it (20)(21)(22)(23)(24)(25)(26)(27)(28)(29)(30)(31)(32)(33). Therefore, we hypothesized that using optical superresolution technology would enable us to study cellular carbon ion radiation effects at the nanometer scale.…”

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confidence: 99%

“…Our microscope setup combines structured illumination microscopy (SIM) and spectral precision distance microscopy (SPDM), allowing subsequent acquisitions of the same cell in both modes (34). SIM uses a special illumination pattern to improve the resolution up to 2-fold (27,28,35). SPDM is a variant of single-molecule localization microscopy; it enables us to detect the position of individual (standard) fluorophores with localization accuracies in the range of 10 to 20 nm (36,37).…”

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Superresolution light microscopy shows nanostructure of carbon ion radiation‐induced DNA double‐strand break repair foci

et al. 2016

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Carbon ion radiation is a promising new form of radiotherapy for cancer, but the central question about the biologic effects of charged particle radiation is yet incompletely understood. Key to this question is the understanding of the interaction of ions with DNA in the cell's nucleus. Induction and repair of DNA lesions including double-strand breaks (DSBs) are decisive for the cell. Several DSB repair markers have been used to investigate these processes microscopically, but the limited resolution of conventional microscopy is insufficient to provide structural insights. We have applied superresolution microscopy to overcome these limitations and analyze the fine structure of DSB repair foci. We found that the conventionally detected foci of the widely used DSB marker gH2AX (Ø 700-1000 nm) were composed of elongated subfoci with a size of ∼100 nm consisting of even smaller subfocus elements (Ø 40-60 nm). The structural organization of the subfoci suggests that they could represent the local chromatin structure of elementary DSB repair units at the DSB damage sites. Subfocus clusters may indicate induction of densely spaced DSBs, which are thought to be associated with the high biologic effectiveness of carbon ions. Superresolution microscopy might emerge as a powerful tool to improve our knowledge of interactions of ionizing radiation with

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Superresolution light microscopy shows nanostructure of carbon ion radiation‐induced DNA double‐strand break repair foci

et al. 2016

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“…Finally, the third aspect of imaging that needs to be addressed is resolution, where we find great efforts directed towards super-resolution techniques, including stimulated emission depletion (STED) [63][64][65][66], structured illumination microscopy (SIM) [67,68], photoactivated localization microscopy (PALM) [69,70], and stochastic optical reconstruction microscopy (STORM) [71][72][73][74]. These approaches offer sub-diffraction-limited resolution and have opened new ways of exploring the submicron world within the living cell.…”

Section: Imaging Smaller: Pushing Resolution To the Limitmentioning

confidence: 99%

“…A technique which offers a superior acquisition time at lower intensities and still gives subdiffraction resolution is (3D) SIM (3D-SIM) [67,68,80,81], which improves the diffraction-limited resolution by a factor of about two (note that STED and PALM/STORM offer higher resolution). In SIM the sample is illuminated by a pattern which is rotated and translated, and all this information is combined to generate an image with twice the resolving power than that obtained with homogeneous illumination, with a slight increase in acquisition times (one image per rotation, typically).…”

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Unleashing Optics and Optoacoustics for Developmental Biology

Ripoll

Koberstein-Schwarz

Ntziachristos

2015

Trends in Biotechnology

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“…Bei der strukturierten Beleuchtung wird die axiale und laterale Ausdehnung der OTF erweitert. Dabei moduliert man die Objektfrequenzen derart, dass höhere Frequenzbänder in niedere Frequenzbereiche verschoben werden und damit übertragbar sind [8,9]. Dieses Konzept wurde in seinen Grundzügen bereits 1963 von Lukosz entwickelt und demonstriert [10].…”

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Strukturierte Beleuchtung in der Hochauflösungsmikroskopie

et al. 2010

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Wer als Zellbiologe hätte sich nicht schon immer gewünscht, mit seinem Lichtmikroskop feinste Details in der Zelle aufzulösen? Denn die Struktur einer biologischen Einheit sagt viel über ihre Funktionsweise und Dynamik aus. Den Strich durch die Rechnung macht nicht die biologische Probe selbst, sondern ein physikalisches Gesetz, welches Ernst Abbe bereits 1873 formulierte [1]. Es besagt, dass die Auflösung eines Weitfeld Lichtmikroskops durch die Beugung fundamental begrenzt ist. Es gibt aber mittlerweile erfolgreiche Ansätze, das Mikroskop und die mikroskopische Abbildung so zu modifizieren, dass eine Auflösung jenseits des Abbeschen Gesetzes möglich wird. Eines dieser Verfahren ist die strukturierte Beleuchtungsmikroskopie (SIM; engl. „Structured Illumination Microscopy”︁).

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<title>Laterally modulated excitation microscopy: improvement of resolution by using a diffraction grating</title>

Cited by 538 publications

References 6 publications

Superresolution light microscopy shows nanostructure of carbon ion radiation‐induced DNA double‐strand break repair foci

Superresolution light microscopy shows nanostructure of carbon ion radiation‐induced DNA double‐strand break repair foci

Unleashing Optics and Optoacoustics for Developmental Biology

Strukturierte Beleuchtung in der Hochauflösungsmikroskopie

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