<title>Holographic high-resolution endoscopic image recording</title>

Abstract

Endoscopic holography or endoholography combines the features of endoscopy and holography. The purpose of endoholographic imaging is to provide the physician with a unique means of extending diagnosis by providing a life-like record of tissue. Endoholographic recording will provide means for microscopic examination of tissue and, in some cases, may obviate the need to excise specimens for biopsy.In this method, holograms, which have the unique properties of three-dimensionality, large focal depth, and high resolution, are made with a newly designed endoscope. The endoscope uses a single-mode optical fiber for illumination and single-beam reflection holograms are recorded in close contact with the tissue at the distal end of the endoscope. The holograms are viewed under a microscope.By using the proper combinations of dyes for staining specific tissue types with various wavelengths of laser illumination, increased contrast on the cellular level can be obtained. Using dyes such as rose bengal in combination with the 514.5 nm line of an argon ion laser, and trypan blue or methylene blue with the 647.1 nm line of a krypton ion laser, holograms of the stained colon of a dog, showed the architecture of the colon's columnar epithelial cells.It is hoped through chronological study using this method in-vivo, an increased understanding of the etiology and pathology of diseases such as Crohn's diseases, colitis, proctitis, and several different forms of cancer, will help lead to their control.

Integrated simultaneous dual-modality imaging endospeckle fluoroscope system for early colon cancer diagnosis

“…integrated into the commercial probe where fiber optics are used for illumination and micro-CCD or other optical imaging is used for microscopic examination of tissues. 7 In this context this proposed technique has real relevance apart from its novelty as illustrated in this paper.…”