<p>Transcriptome Changes In Dorsal Spinal Cord Of Rats With Neuropathic Pain</p>

Abstract

BackgroundMechanisms of neuropathic pain are not fully understood. Molecular changes in spinal dorsal horn take part in the initiation and development of neuropathic pain.MethodsTo detect the transcriptome changes in the dorsal spinal cord of neuropathic pain rat, sciatic nerve chronic constriction injury (CCI) rats were used. Then, the CCI ipsilateral dorsal spinal cords of lumbar L3-L5 segments were collected at 14th day post-CCI and subjected to microRNA and long non-coding RNA (lncRNA)/mRNA microarray. To evaluate functions of differential mRNAs, bioinformatics methods including gene ontology (GO) and KEGG pathway analysis were conducted for significantly up- and downregulated mRNAs.ResultsMicroRNA microarrays showed that 13 microRNAs were differently expressed between CCI and sham-operated rats (fold change ≥ 2.0). Six of them were upregulated, and the other seven were downregulated in CCI group. MicroRNA-1b overexpressed 18.7 times after CCI. LncRNA/mRNA microarray detected 876 lncRNAs with significant differential expression (fold change ≥ 2.0). Among them, 339 were significantly upregulated, and 537 were downregulated in CCI group. Sixteen of them differentially expressed more than 10 times and the lncRNA XR_356687 overexpressed as high as 53 times. In addition, 950 mRNAs were differentially expressed (fold change ≥ 2.0), including 405 upregulated and 545 downregulated in CCI group. Ten of these mRNAs with changed expressions of more than 10 times. The Hspa1b (encodes heat shock protein 70) overexpressed 24 times in CCI rats. Gene ontology analysis revealed that hundreds of differentially expressed mRNAs involved in the biological processes, cellular component, and molecular function. In addition, these genes significantly enriched into 32 KEGG pathways, including the TNF, FoxO, cytokine–cytokine receptor interaction, and calcium signaling pathways.ConclusionNeuropathic pain induced comprehensive changes of transcription profile in the dorsal spinal cord. These differentially expressed transcripts in spinal cord could be potential targets in defeating neuropathic pain.

Microarray Analysis of Potential Biomarkers for brachial plexus avulsion caused neuropathic pain

“…Some authors suggested that there is a high possibility of neuropathic pain caused by nerve damage[18,19]. The transcriptome changed play an important role in neuropathic pain[13]. So our research is meaningful and feasible.…”

Systemic gene expression profiles according to pain types in individuals with chronic spinal cord injury

“… 37 BCL7B and HSPA1B were both elevated in a rat chronic constriction model of neuropathic pain. 38 , 39 It was previously suggested in several publications that PTPRC/CD45, a gene identified as significantly differentially expressed following SCI in preclinical models, may be important in the development of neuropathic pain and a possible target of pain intervention. 40 – 42 Other genes, IFITM1 and IRF1, both of which are regulated by the pro-inflammatory cytokine interferon gamma, were significantly upregulated in preclinical models of SCI models.…”

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“…The chronic constrictions injury (CCI) was surgically triggered on the right hind limb of the SD rats as described in previously reported protocol. 44,45 The animals were anesthetized with 4% chloroform for initiation for maintenance. A 15 mm of skin incitation was created via rounded partitions of the biceps of the female muscles.…”