<p>The Extract from <em>Acidosasa longiligula</em> Alleviates in vitro UV-Induced Skin Cell Damage via Positive Regulation of Thioredoxin 1</p>

Abstract

Introduction: Skin, as the outermost organ, is exposed to a wide range of environmental risk factors including ultraviolet (UV) and all kinds of pollutants. Excessive UV exposure contributes to many disorders, such as photoaging, skin inflammation, and carcinogenesis. Methods: To determine the effects of bamboo extract (BEX) from our local plant, Acidosasa longiligula, on UV-irritated human skin, we conducted a variety of studies, including Western blot, apoptosis assays, reactive oxygen species (ROS) detection, and thioredoxin (TXN) and thioredoxin reductase (TXNRD) activity assays in primary skin keratinocytes.Results: We first determined that BEX protects human skin keratinocytes against UV radiation-induced apoptosis and ROS production. UV radiation can robustly impair TXN and TXNRD activity which can, in turn, be significantly rescued by BEX treatment. Moreover, BEX regulates TXN1 levels in primary skin keratinocytes and TXN1 is proved to be required for the protective function of BEX. Last, we found that the NF-κB/p65 pathway mediates the protective function of BEX against UV. Discussion: Collectively, our work delineates the beneficial role of BEX in UV-induced skin cell damage and provides a novel therapeutic reagent to prevent or alleviate the progress of photoaging and other UV-provoked skin diseases.

A novel peptide ‘T14’ reflects age and photo-aging in human skin

“…Nonetheless this outlier showing high T14 in the older age group prompts the questions: is the peptide a direct and inflexible marker for calendar age alone? Epidermal cell growth and proliferation is highly multifactorial and influenced by a range of environmental factors which include, for example, UV and even pollutants [ 10 , 16 , 28 ]. The second part of this study showed a significant difference, within the same age range, of photo-protected versus photo-exposed skin, such that T14 declined as an index not only of age, but of aging.…”

“…Immunohistochemistry was performed on young photoprotected (ages [16][17][18][19][20][21][22][23][24][25][26][27][28][29][30][31], middle photo protected (ages 37-45), aged photo protected (ages 50-70), and aged photo-exposed (ages 48-83) skin samples. All samples were paraffin embedded and sectioned to generate 5µm thick samples.…”

Thioredoxin domain-containing protein 9 protects cells against UV-B-provoked apoptosis via NF-κB/p65 activation in cutaneous squamous cell carcinoma

“…Individuals under excessive UV radiation, particularly UV-B (275-320 nm), are at greater risk of skin damage, including solar erythema, photoaging, and even skin cancers [13]. UV-B radiation is known to generate reactive oxygen species (ROS), such as singlet oxygen, superoxide anion, and hydrogen peroxide, leading to a redox imbalance in skin cells [14][15][16]. However, tumor cells can tolerate stressed environments by upregulating the expression of some antioxidant genes.…”