&lt;p&gt;The Effects Study of Isoniazid Conjugated Multi-Wall Carbon Nanotubes Nanofluid on &lt;em&gt;Mycobacterium tuberculosis&lt;/em&gt;&lt;/p&gt;

Zomorodbakhsh, Shahab; Abbasian, Yasamin; Naghinejad, Maryam; Sheikhpour, Mojgan

doi:10.2147/ijn.s251524

Cited by 38 publications

(14 citation statements)

References 33 publications

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“… 64 Future investigations of nanotechnology-based CS formulations in our experimental systems of macrophage polarization and LM biosynthesis might reveal if cytotoxicity and SPM-impairing effects may be circumvented. Indeed, drug delivery by nanofluids improved the efficacy of isoniazid 65 or of bromocriptine, 66 and encapsulation of a cytotoxic indirubin derivative into polymer-based nanoparticles reduced its detrimental impact on monocyte viability. 67 …”

Section: Discussionmentioning

confidence: 99%

Modulation of Inflammation-Related Lipid Mediator Pathways by Celastrol During Human Macrophage Polarization

Zhang¹,

Jordan²,

Hofstetter³

et al. 2022

JIR

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Background and Purpose Celastrol (CS) is a major active ingredient of the Chinese/Asian herb Tripterygium wilfordii that is frequently used as phytomedicine to treat inflammation and autoimmune diseases. We showed before that short-term exposure to CS (1 µM) favorably impacts the biosynthesis of inflammation-related lipid mediators (LM) in human polarized macrophages by modulating the activities of different lipoxygenases (LOXs). However, whether CS regulates the expression of LOXs and other related LM-biosynthetic enzymes during macrophage polarization is unknown. Here, we investigated how CS affects LM-biosynthetic enzyme expression on the protein level and studied concomitant LM signature profiles during polarization of human monocyte-derived macrophages (MDM) towards M1- and M2-like phenotypes. Methods and Results We used LM metabololipidomics to study the long-term effects of CS on LM profile signatures after manipulation of human monocyte-derived macrophages (MDM) during polarization. Exposure of MDM to low concentrations of CS (ie, 0.2 µM) during polarization to an inflammatory M1 phenotype potently suppressed the formation of pro-inflammatory cyclooxygenase (COX)- and 5-LOX-derived LM, especially prostaglandin (PG)E 2 . Notably, gene and enzyme expression of COX-2 and microsomal PGE 2 synthase (mPGES)-1 as well as M1 markers were strongly decreased by CS during M1-MDM polarization, along with impaired activation of nuclear factor-κB and p38 mitogen-activated protein kinase. During IL-4-induced M2 polarization, CS decreased the capacity of the resulting M2-MDM to generate pro-inflammatory COX and 5-LOX products as well but it also reduced the formation of 12/15-LOX products and specialized pro-resolving mediators, without affecting the levels of liberated fatty acid substrates. Conclusion Depending on the timing and concentration, CS not only favorably affects LOX activities in macrophages but also the expression of LM-biosynthetic enzymes during macrophage polarization connected to changes of inflammation-related LM which might be of relevance for potential application of CS to treat inflammatory disorders.

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Section: Discussionmentioning

confidence: 99%

Modulation of Inflammation-Related Lipid Mediator Pathways by Celastrol During Human Macrophage Polarization

Zhang¹,

Jordan²,

Hofstetter³

et al. 2022

JIR

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“…For nanofluid preparation, 0.2 g of nano-drug powder, 6 ml of 96% ethanol, and 0.06 g of Arabic gum were added to 100 ml of deionized water. The mixture was stirred for 20 min and was placed in an ice container, and then, sonication was carried out for 20 min at 200 W 19 .…”

Section: Methodsmentioning

confidence: 99%

A success targeted nano delivery to lung cancer cells with multi-walled carbon nanotubes conjugated to bromocriptine

Kamazani

Nematalahi

Siadat

et al. 2021

Sci Rep

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In this research, a new nano drug-based multi-walled carbon nanotubes (MWCNTs) was prepared and evaluated qualitatively. Bromocriptine (BRC) was conjugated to functionalized carbon nanotubes. Then, the CHNS, FT-IR, SEM, and RAMAN tests for characterization of the conjugated drug were done. The nanofluid-containing nano-drug was evaluated on lung cancer cells (A549 & QU-DB) and MRC5 by MTT and flow cytometry tests. Then, the gene expression studies of dopamine receptor genes were done before and after nano-drug treatment. After that, a western blotting test was carried out for further investigation of dopamine receptors protein production. Finally, Bax and Bcl-2 secretion were measured by the ELISA method in cells affected by MWCNTs-BRC Nf compared to untreated cells. The results showed that the nano-drug had a significant lethal effect on cancer cells, while it had no toxicity on MRC5. Also, the nano-drug could significantly induce apoptosis in lung cancer cells at a lower dose compared to the drug alone. In this study, a targeted nano-drug delivery system was designed, and its performance was evaluated based on neurotransmitter pathways, and the results showed that it may be useful in the treatment of lung cancer. However, additional studies on animal models are underway.

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“…Another study in 2020 evaluated the effects of isoniazid-functionalized MWCNTs on M. tuberculosis . Their results showed that isoniazid-functionalized MWCNTs exerted antimicrobial effects at lower concentrations compared to the usual doses of antibiotics [ 32 ]. In a recent study.…”

Section: Conclusion and Implications For Future Researchmentioning

confidence: 99%

Antibiotic delivery evaluation against Mycobacterium fortuitum using nanofluids containing carbon nanotubes

et al. 2022

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Background Mycobacterium fortuitum (M. fortuitum) is a bacterium, which can cause infections in many anatomical regions of the body, including the skin, lymph nodes, and joints. This bacterium, which belongs to a group of bacteria known as nontuberculous mycobacteria, is regarded as an important nosocomial pathogen worldwide owing to its increasing antibiotic resistance. Recently, the antimicrobial effects of carbon nanotubes have been reported in numerous studies. These nanotubes can be very useful in drug delivery; besides, they exhibit unique properties against multidrug-resistant bacterial infections. This study aimed to investigate the antimicrobial effects of carboxyl-functionalized multi-walled carbon nanotubes (MWCNT-COOH) to reduce antibiotic resistance. Methods In this study, antibacterial effects of nanofluids containing functionalized MWCNTs at initial concentration of 2 mg/mL and serial dilutions of 54, 28.5, 14.25, 7.12, 3.5 µg/mL, antibiotics alone and combination of nanofluids with antibiotics were investigated. Standard and resistant strains of M. fortuitum were obtained from the microbial bank of the Department of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran. Results It was observed that nanofluid containing MWCNT-COOH can exert antimicrobial effects on M. fortuitum and significantly reduce bacterial resistance to antibiotics including kanamycin and streptomycin. In the presence of antibiotics and nanofluids containing MWCNT-COOH at a dose of 28.5 µg/mL, no growth was observed. Conclusion One of the main antimicrobial mechanisms of MWCNT-COOH is penetration into the bacterial cell wall. In this study, by using the nanofluid containing MWCNT-COOH with increased stability, the antibiotic resistance of M. fortuitum was significantly reduced at lower dilutions compared to the antibiotic alone.

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<p>The Effects Study of Isoniazid Conjugated Multi-Wall Carbon Nanotubes Nanofluid on <em>Mycobacterium tuberculosis</em></p>

Cited by 38 publications

References 33 publications

Modulation of Inflammation-Related Lipid Mediator Pathways by Celastrol During Human Macrophage Polarization

Modulation of Inflammation-Related Lipid Mediator Pathways by Celastrol During Human Macrophage Polarization

A success targeted nano delivery to lung cancer cells with multi-walled carbon nanotubes conjugated to bromocriptine

Antibiotic delivery evaluation against Mycobacterium fortuitum using nanofluids containing carbon nanotubes

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